##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630845.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	443984
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46917906951602	31.0	31.0	34.0	28.0	34.0
2	31.472377382968755	31.0	31.0	34.0	28.0	34.0
3	30.954782154311868	31.0	31.0	34.0	27.0	34.0
4	35.08931402933439	37.0	35.0	37.0	32.0	37.0
5	35.28278271289055	37.0	35.0	37.0	33.0	37.0
6	35.48276964935673	37.0	35.0	37.0	33.0	37.0
7	35.4780329921799	37.0	35.0	37.0	33.0	37.0
8	35.50024775667592	37.0	35.0	37.0	33.0	37.0
9	37.27536127428015	39.0	37.0	39.0	34.0	39.0
10	37.10431456989441	39.0	37.0	39.0	33.0	39.0
11	37.15500333345346	39.0	37.0	39.0	33.0	39.0
12	37.0717953800137	39.0	37.0	39.0	33.0	39.0
13	37.15416096075534	39.0	37.0	39.0	33.0	39.0
14	38.277904158708424	40.0	38.0	41.0	33.0	41.0
15	38.2757937222963	40.0	38.0	41.0	33.0	41.0
16	38.28355526325273	40.0	38.0	41.0	33.0	41.0
17	38.27059758910231	40.0	38.0	41.0	33.0	41.0
18	38.316531676817185	40.0	38.0	41.0	33.0	41.0
19	38.351598255793	40.0	38.0	41.0	34.0	41.0
20	38.333180925438754	40.0	38.0	41.0	34.0	41.0
21	38.305389383401206	40.0	38.0	41.0	34.0	41.0
22	38.21602580273163	40.0	38.0	41.0	34.0	41.0
23	38.22384139969008	40.0	38.0	41.0	34.0	41.0
24	38.1691660059822	40.0	38.0	41.0	33.0	41.0
25	38.131675916249236	40.0	37.0	41.0	33.0	41.0
26	38.05565515874446	40.0	37.0	41.0	33.0	41.0
27	37.96607310173339	40.0	37.0	41.0	33.0	41.0
28	37.96560686871599	40.0	37.0	41.0	33.0	41.0
29	37.885779667735775	40.0	37.0	41.0	33.0	41.0
30	37.77910465241991	40.0	37.0	41.0	33.0	41.0
31	37.74086678799236	40.0	37.0	41.0	33.0	41.0
32	37.623049479260516	40.0	37.0	41.0	32.0	41.0
33	37.591052830732636	40.0	37.0	41.0	32.0	41.0
34	37.511966647446755	40.0	36.0	41.0	32.0	41.0
35	37.47529415474432	40.0	36.0	41.0	32.0	41.0
36	37.44459485026488	40.0	36.0	41.0	32.0	41.0
37	37.36537352697395	39.0	36.0	41.0	32.0	41.0
38	37.323335976071206	39.0	36.0	41.0	32.0	41.0
39	37.20798272009802	39.0	36.0	41.0	31.0	41.0
40	37.08697835958053	39.0	36.0	41.0	31.0	41.0
41	37.02442655591192	39.0	36.0	41.0	31.0	41.0
42	36.947088183357955	39.0	36.0	41.0	31.0	41.0
43	36.283602562254494	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	9.0
11	13.0
12	20.0
13	4.0
14	9.0
15	11.0
16	8.0
17	6.0
18	9.0
19	24.0
20	38.0
21	80.0
22	177.0
23	357.0
24	582.0
25	1062.0
26	1649.0
27	2644.0
28	3879.0
29	5522.0
30	7717.0
31	10053.0
32	13078.0
33	17060.0
34	21978.0
35	29906.0
36	41426.0
37	60826.0
38	103455.0
39	122372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.238990594255654	23.165249198169303	15.335237305848858	23.260522901726187
2	13.693511477891096	22.669961079678547	41.436853580309204	22.19967386212116
3	14.621923312551804	29.33461566182565	33.98140473530577	22.062056290316768
4	10.868860139104111	19.434484125554075	38.04686655374969	31.64978918159213
5	12.289406825471188	38.42007820101625	35.4274027892897	13.863112184222857
6	27.042866409600347	40.423754009153484	17.717530361454468	14.815849219791705
7	24.597733251648705	33.24106274099968	22.429411870698043	19.731792136653574
8	24.77319002486576	34.70372806227252	22.589552776676637	17.93352913618509
9	23.44476377527118	14.758414717647483	19.84823777433421	41.948583732747124
10	14.336552668564634	28.96433204800173	33.70346679159609	22.995648491837546
11	31.423429673141374	23.673150383797616	24.786028325345058	20.117391617715956
12	20.67777217197016	26.474152221701686	29.59768820498036	23.250387401347798
13	29.17492522253054	21.185222890914986	26.698034163393274	22.941817723161193
14	21.526451403654185	22.699691880788496	26.971242206926377	28.802614508630942
15	23.49206277703701	29.161636455367763	23.70107931817363	23.645221449421598
16	24.349526109048973	27.659780532631807	23.471116076255	24.519577282064215
17	21.19625932466035	30.10356229053299	24.952926231575912	23.747252153230747
18	23.254441601499153	24.981981332660634	27.320128653284804	24.44344841255541
19	23.915276226170313	27.67870013333814	27.633878698331472	20.77214494216008
20	22.290668132184944	27.555722728746986	26.070083606616457	24.08352553245162
21	24.397050344156547	25.61105805614617	26.509288623013443	23.482602976683843
22	22.563650942376302	29.8528775811741	26.593075426141482	20.990396050308117
23	22.433240837507658	25.193024973872934	27.61090489747378	24.76282929114563
24	23.128310930123607	27.882536307614693	28.221287253594724	20.76786550866698
25	22.271973764820356	28.60395870121446	26.41874481963314	22.70532271433205
26	23.622472881905654	25.752729828101913	27.9699268442106	22.65487044578183
27	22.301028865905078	30.111895924177446	26.33090381635374	21.256171393563733
28	20.44623229665934	25.165771739522146	28.656438069840355	25.731557893978163
29	23.345435871562938	26.074137806767812	29.34903059569714	21.231395725972106
30	21.953268586255362	27.454818191646545	27.769919636743666	22.821993585354427
31	22.07309272406213	24.64660888680673	28.011144545749396	25.26915384338174
32	25.07049803596526	26.134500342354677	27.515631193916896	21.279370427763165
33	20.73498144077264	25.022973800857688	29.184159789541965	25.057884968827704
34	21.309326462214855	27.854607373238675	29.167492522253053	21.668573642293413
35	22.908032721899886	25.891924033298498	30.4983963386068	20.701646906194817
36	22.487747306209233	25.642590723990054	25.850706331759703	26.018955638041007
37	21.95642185303975	26.874617103319036	28.938880680384877	22.230080363256334
38	21.598751306353382	24.824543226782946	28.728287505856066	24.848417961007605
39	23.834867923168403	25.67502432520091	27.536352661357167	22.95375509027352
40	21.260901293740314	25.343255612814875	31.844165555515513	21.551677537929294
41	22.544731341669973	26.462665321272837	26.514018523190025	24.478584813867165
42	22.07759739089697	26.091706007423692	29.875400915348298	21.955295686331038
43	23.544316912321165	24.427231611949978	28.98663014883419	23.041821326894663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	8.0
2	14.0
3	55.0
4	96.0
5	96.0
6	152.5
7	209.0
8	215.0
9	221.0
10	342.5
11	464.0
12	464.0
13	656.0
14	848.0
15	2230.5
16	3613.0
17	4007.5
18	4402.0
19	4402.0
20	4321.0
21	4240.0
22	4956.0
23	5672.0
24	6952.0
25	8232.0
26	8232.0
27	9717.5
28	11203.0
29	12912.5
30	14622.0
31	16608.5
32	18595.0
33	18595.0
34	22263.0
35	25931.0
36	26675.0
37	27419.0
38	27588.0
39	27757.0
40	27757.0
41	27755.5
42	27754.0
43	28354.0
44	28954.0
45	28916.5
46	28879.0
47	28879.0
48	28865.5
49	28852.0
50	29117.0
51	29382.0
52	36663.0
53	43944.0
54	43944.0
55	35091.0
56	26238.0
57	27902.5
58	29567.0
59	24468.5
60	19370.0
61	19370.0
62	14840.0
63	10310.0
64	7991.5
65	5673.0
66	4777.0
67	3881.0
68	3881.0
69	3238.5
70	2596.0
71	2557.0
72	2518.0
73	2179.0
74	1840.0
75	1840.0
76	1097.0
77	354.0
78	247.5
79	141.0
80	114.0
81	87.0
82	87.0
83	73.5
84	60.0
85	40.5
86	21.0
87	18.0
88	15.0
89	15.0
90	10.0
91	5.0
92	3.0
93	1.0
94	1.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	443984.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.09868465606017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.07083651272218	47.723620309544515
2	9.181558592611484	10.301467202761026
3	2.2342776614126727	3.7602011388500736
4	0.912862745462235	2.04841597167805
5	0.5231933355329661	1.4675228972108072
6	0.33419830441043913	1.1248851175026735
7	0.24480465089346384	0.9613253238966529
8	0.19155549475803307	0.8596809035653188
9	0.14095369867535484	0.7116585383784653
>10	0.9929478083194705	10.952857426721721
>50	0.10271994120469355	4.017447055457345
>100	0.06163196472151729	6.164243490663449
>500	0.004431056287167909	1.620266362198729
>1k	0.002819763091834124	3.467641189391841
>5k	0.0012084698965003388	4.818767072179316
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	7815	1.7601985657140797	No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG	7813	1.7597480990305958	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	5696	1.2829291145626869	Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC	4281	0.9642239359976936	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2380	0.5360553533460665	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2254	0.5076759522865689	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	2089	0.4705124508991315	No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC	1845	0.4155555155140726	Illumina PCR Primer Index 11 (95% over 23bp)
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1387	0.3123986449962161	No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG	1109	0.24978377599192766	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	964	0.21712494143933114	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	816	0.18379040686150852	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	789	0.1777091066344733	No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA	689	0.15518577246026885	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	668	0.1504558722836859	No Hit
ACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT	580	0.13063533821038595	No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT	571	0.12860823813470754	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	552	0.12432880464160871	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	524	0.11802227107283146	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	509	0.11464377094670078	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	508	0.11441853760495874	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	491	0.11058957079534398	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	4.5046668348408954E-4	0.0
17	0.0	0.0	0.0	6.757000252261342E-4	0.0
18	0.0	0.0	0.0	6.757000252261342E-4	0.0
19	0.0	0.0	0.0	9.009333669681791E-4	0.0
20	0.0	0.0	0.0	0.0013514000504522684	0.0
21	0.0	0.0	0.0	0.0024775667591624925	0.0
22	0.0	0.0	0.0	0.0031532667843886266	0.0
23	0.0	0.0	0.0	0.004504666834840895	0.0
24	0.0	0.0	0.0	0.004955133518324985	0.0
25	0.0	0.0	0.0	0.004955133518324985	0.0
26	0.0	0.0	0.0	0.005180366860067029	0.0
27	0.0	0.0	0.0	0.0058560668852931635	0.0
28	0.0	0.0	0.0	0.010811200403618148	0.0
29	0.0	0.0	0.0	0.020946700782010164	0.0
30	0.0	0.0	0.0	0.035136401311758984	0.0
31	0.0	0.0	0.0	0.06959710259829183	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGGG	45	2.6120688E-9	37.0	3
CGTCTTC	295	0.0	33.864407	37
GATTACG	45	1.0774238E-7	32.88889	1
CTTATAC	955	0.0	32.157066	1
GCCTACC	30	3.5959514E-4	30.833334	1
TATACAC	1075	0.0	29.255816	3
TTATACA	1090	0.0	28.85321	2
GGTATCA	305	0.0	27.90164	1
GTATCAA	970	0.0	25.36598	1
CCGTCTT	920	0.0	24.331522	37
GCCGTCT	965	0.0	23.196892	36
ATTACCC	80	2.7162969E-8	23.125	3
CGCAAGT	40	0.0019293305	23.125	12
ATTACGG	75	3.7300742E-7	22.2	2
CGGAGTT	50	2.6984836E-4	22.2	24
ATTACCG	60	3.7196583E-5	21.583334	5
GCACGGA	60	3.7196583E-5	21.583334	21
TTACCGC	60	3.7196583E-5	21.583334	6
ATAGCGG	70	5.0902927E-6	21.142857	7
TCGGGAT	70	5.0902927E-6	21.142857	22
>>END_MODULE