FastQCFastQC Report
Fri 10 Feb 2017
ERR1630844.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630844.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156561
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT92685.919737354769068RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG92565.912072610675711No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT77974.9801674746584395No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC55783.5628285460619185No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC32052.047125401600654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26741.7079604754696252No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC25621.6364228639316307RNA PCR Primer, Index 46 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23011.4697146799011247No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG13790.8808068420615607No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11170.7134599293566085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9180.5863529231417786No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT7640.48798870727703575No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA7610.4860725212536966No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7140.45605227355471667No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT6830.43625168464687886No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6400.4087863516456844No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6240.3985666928545423No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4810.3072284924087097No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4570.29189900422199655No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4500.2874279035008719No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4320.275930787360837No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4210.2689047719419268No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3970.2535752837552136No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3500.22355503605623367No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT3410.21780647798621622No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3310.2114191912417524No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3260.2082255478695205No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.2075868191950741No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3080.19672843172948562No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3010.19225733100836095No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2820.1801214861938797No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2750.17565038547275502No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2540.162237083309381No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2510.16032089728604185No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2480.15840471126270272No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2450.15648852523936357No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2390.1526561531926853No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.15073996716934612No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2330.14882378114600697No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2240.14307522307598955No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2230.14243649440154316No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2210.1411590370526504No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2150.1373266650059721No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2140.13668793633152573No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2130.13604920765707937No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2100.1341330216337402No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2090.13349429295929383No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA2040.13030064958706192No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2030.12966192091261552No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2000.1277457348892764No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1990.12710700621483No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1940.12391336284259809No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC1900.12135844814481257No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1890.12071971947036618No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1860.11880353344702703No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1820.11624861874924151No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG1780.11369370405145598No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG1780.11369370405145598No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG1770.1130549753770096No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1750.11177751802811683No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1750.11177751802811683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC1720.1098613320047777No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG1650.10539023128365302No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1620.10347404526031387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCGT200.001837084337.037
TTACACA251.2287608E-436.9999963
TTTACAC251.2287608E-436.9999962
CTTATAC8300.035.662651
CGTCTTC2600.034.15384737
TTATACA9250.032.02
GACAGAC303.585416E-430.83333416
GTCTTAG303.585416E-430.8333341
TATACAC10050.030.0049743
TAAGCCG250.005482032429.59999836
ATTACTT250.005482032429.5999982
GTAAGCC250.005482032429.59999835
CCGTCTT10600.027.92452837
GCCGTCT10750.027.53488536
GGTATCA2850.026.6140371
ACAGACA358.8350737E-426.4285717
TATAAAA358.8350737E-426.428572
GACACAT358.8350737E-426.4285720
CAGACAC358.8350737E-426.4285718
AGACAGA358.8350737E-426.4285715
Produced by FastQC (version 0.11.5)