FastQCFastQC Report
Fri 10 Feb 2017
ERR1630843.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630843.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273648
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58440.4588394909739582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52960.415813474366544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42100.33054658744017185No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30660.24072585204075225No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC26790.21034069067748704No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT22250.1746950491815635No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA21300.1672361594412271No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA20970.16464517668932074No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG17930.14077672952024423No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG15390.12083401379345No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA14660.11510244588771781No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG14620.11478838737233521No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG14320.11243294850696581No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG13250.10403188322048165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAG952.8194336E-1023.36842235
ATTCGCG450.00382603120.55555711
ACGTATT450.00382603120.55555731
TAACGCT1052.2599124E-819.3809532
GTGTTAT1002.8755312E-718.51
TAGCGAT500.00703528818.54
GCTTTAT6700.018.2238831
ATTGCCG953.6070069E-617.52631611
GGTATCA27900.017.372761
CAATACG752.0674881E-417.2666664
ACGTTAA1301.3933459E-817.07692320
TTTAGAC1201.041426E-716.9583323
ACGCTAA1201.041426E-716.95833234
TATTGAG7250.016.841385
CATAATA1005.878792E-616.6500022
GAGTATA1251.6580088E-716.281
TACGTTA1251.6580088E-716.2819
TATACTG1606.2937033E-1016.18755
GCTCGTA803.382924E-416.18759
GCACCGT700.002592699815.8571426
Produced by FastQC (version 0.11.5)