##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630843.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1273648
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10037545695514	33.0	31.0	34.0	30.0	34.0
2	32.2695140258533	34.0	31.0	34.0	30.0	34.0
3	32.37519785686469	34.0	31.0	34.0	30.0	34.0
4	35.939805189502906	37.0	35.0	37.0	35.0	37.0
5	35.89411674183134	37.0	35.0	37.0	35.0	37.0
6	35.96881948544653	37.0	35.0	37.0	35.0	37.0
7	35.91590612162858	37.0	35.0	37.0	35.0	37.0
8	35.92012471263646	37.0	35.0	37.0	35.0	37.0
9	37.62550641935606	39.0	37.0	39.0	35.0	39.0
10	37.55239359697499	39.0	37.0	39.0	35.0	39.0
11	37.63862542868987	39.0	37.0	39.0	35.0	39.0
12	37.590836714696685	39.0	37.0	39.0	35.0	39.0
13	37.62945020916297	39.0	37.0	39.0	35.0	39.0
14	38.93930740675603	40.0	38.0	41.0	36.0	41.0
15	38.94734808989611	40.0	38.0	41.0	36.0	41.0
16	38.904293022876026	40.0	38.0	41.0	35.0	41.0
17	38.90512056706405	40.0	38.0	41.0	35.0	41.0
18	38.89497412157833	40.0	38.0	41.0	35.0	41.0
19	38.944724131000086	40.0	38.0	41.0	35.0	41.0
20	38.91828590002889	40.0	38.0	41.0	35.0	41.0
21	38.87811389018002	40.0	38.0	41.0	35.0	41.0
22	38.8108472670628	40.0	38.0	41.0	35.0	41.0
23	38.7699285830936	40.0	38.0	41.0	35.0	41.0
24	38.71919242993354	40.0	38.0	41.0	35.0	41.0
25	38.674998900795195	40.0	38.0	41.0	34.0	41.0
26	38.53756453902491	40.0	38.0	41.0	34.0	41.0
27	38.4093477946811	40.0	38.0	41.0	34.0	41.0
28	38.32083275755939	40.0	38.0	41.0	34.0	41.0
29	38.2559105812595	40.0	38.0	41.0	34.0	41.0
30	38.16634501840383	40.0	38.0	41.0	34.0	41.0
31	38.114091962614474	40.0	38.0	41.0	33.0	41.0
32	38.03758495282841	40.0	37.0	41.0	33.0	41.0
33	37.94639099657048	40.0	37.0	41.0	33.0	41.0
34	37.89267835383089	40.0	37.0	41.0	33.0	41.0
35	37.81989607803726	40.0	37.0	41.0	33.0	41.0
36	37.78122134216047	40.0	37.0	41.0	33.0	41.0
37	37.642285780686656	40.0	37.0	41.0	33.0	41.0
38	37.50426334434632	40.0	37.0	41.0	32.0	41.0
39	37.43763111943017	40.0	37.0	41.0	32.0	41.0
40	37.24807246586184	40.0	36.0	41.0	31.0	41.0
41	37.18350124995289	40.0	36.0	41.0	31.0	41.0
42	37.03709737698328	40.0	36.0	41.0	31.0	41.0
43	36.25815295905933	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	5.0
11	3.0
12	2.0
13	2.0
14	2.0
15	3.0
16	5.0
17	9.0
18	27.0
19	48.0
20	114.0
21	284.0
22	511.0
23	897.0
24	1548.0
25	2535.0
26	3892.0
27	5865.0
28	8921.0
29	12283.0
30	16921.0
31	22254.0
32	29199.0
33	37947.0
34	50252.0
35	67157.0
36	95412.0
37	151831.0
38	279866.0
39	485845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.644517166438455	18.732962325540495	11.995700538924412	24.62681996909664
2	19.12098162129568	20.637413162820497	33.4700796452395	26.77152557064432
3	21.221954574576333	22.836921975302438	28.851613632652036	27.089509817469192
4	14.710029772747257	14.807937514917779	33.95404381744407	36.527988894890896
5	14.709480170345337	36.82760071856588	33.85676419230431	14.606154918784467
6	35.82017951584739	33.60787281886361	15.22037486024396	15.351572805045036
7	29.609829403414444	30.16956019245506	21.073561926058062	19.147048478072435
8	29.00762220016834	31.469840960767808	19.746586183937794	19.775950655126064
9	26.968518774418047	13.683293971332738	20.034106754770548	39.31408049947866
10	18.11607288670025	26.689320754242928	32.15974900443451	23.03485735462231
11	35.45131778953055	21.99139793726367	20.933413313568582	21.623870959637202
12	22.99175282338605	25.749422132331695	28.00239940705752	23.25642563722473
13	30.950074117809628	19.52226988932578	25.102854163787796	24.424801829076795
14	23.258467017574713	20.74756918709094	25.297570443325	30.69639335200935
15	26.08813424117182	27.527228873283672	22.481486250518195	23.903150635026318
16	25.08856450133789	26.0153511802319	24.348093036694596	24.547991281735612
17	24.152042008467017	26.146549125032976	25.223374119065866	24.47803474743414
18	23.389900506262325	24.717504365413365	27.38629511450573	24.506300013818574
19	25.04977827468814	24.76657600844189	27.39532429682298	22.788321420046984
20	25.455698905820135	24.295331206110323	26.428966244990765	23.82000364307878
21	25.163781515772023	24.489419368616762	26.528051706593974	23.81874740901725
22	25.1677072472143	24.816589827016568	26.66890695074306	23.346795975026065
23	24.59714143939299	24.754406240970816	26.21658417396329	24.4318681456729
24	23.95073050010678	25.368626182430308	26.931930957375975	23.748712360086934
25	24.40737158147306	25.442822506689446	26.16986796980013	23.97993794203736
26	24.30333969825258	25.6888873534917	26.37887391178724	23.628899036468475
27	24.44560820572089	24.73721153725362	26.359794856977754	24.457385400047738
28	23.839475271032498	25.40168084117433	26.45244211901561	24.306401768777558
29	24.019666344233258	25.80508900418326	26.85161049206688	23.3236341595166
30	24.275467005012374	25.63180721832092	26.066228659723876	24.02649711694283
31	23.265140761026597	26.321087144956852	26.791860859515342	23.621911234501212
32	23.234127482632562	25.5203164453601	26.48855884828461	24.756997223722724
33	22.707529866964812	26.265341758476442	26.887491677449344	24.139636697109406
34	24.731558483976734	24.772307576347625	26.441764129492608	24.054369810183033
35	23.519371129228798	25.067679610064946	26.819419494240165	24.593529766466087
36	22.875629687323343	25.858243407911765	27.150751227968794	24.1153756767961
37	23.524317550846074	24.698346795975027	27.51474504729721	24.262590605881687
38	23.728063012700527	25.06713000766303	26.906963305403064	24.297843674233384
39	23.483568458475183	24.708318153838423	27.445730688541893	24.362382699144504
40	22.62061417283268	24.584500584148838	27.98999409569991	24.80489114731857
41	21.79047900204766	24.975581950429003	28.488483474240923	24.745455573282413
42	22.37195834327852	24.826247126364585	28.791785485471657	24.010009044885244
43	22.735245530947328	24.580103764933483	28.234567164554097	24.450083539565092
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	368.0
1	316.5
2	265.0
3	582.0
4	899.0
5	899.0
6	1318.0
7	1737.0
8	1652.5
9	1568.0
10	2435.5
11	3303.0
12	3303.0
13	5775.5
14	8248.0
15	10988.5
16	13729.0
17	13174.0
18	12619.0
19	12619.0
20	15020.5
21	17422.0
22	16076.0
23	14730.0
24	16803.5
25	18877.0
26	18877.0
27	21042.5
28	23208.0
29	26394.0
30	29580.0
31	33355.5
32	37131.0
33	37131.0
34	40893.5
35	44656.0
36	48579.0
37	52502.0
38	56307.0
39	60112.0
40	60112.0
41	62241.0
42	64370.0
43	65794.5
44	67219.0
45	69793.0
46	72367.0
47	72367.0
48	76910.0
49	81453.0
50	81455.0
51	81457.0
52	82694.0
53	83931.0
54	83931.0
55	81212.0
56	78493.0
57	77652.5
58	76812.0
59	78553.5
60	80295.0
61	80295.0
62	74402.5
63	68510.0
64	63339.0
65	58168.0
66	50393.0
67	42618.0
68	42618.0
69	37837.0
70	33056.0
71	27681.5
72	22307.0
73	16668.5
74	11030.0
75	11030.0
76	7762.5
77	4495.0
78	3590.0
79	2685.0
80	2124.5
81	1564.0
82	1564.0
83	1186.0
84	808.0
85	661.5
86	515.0
87	435.0
88	355.0
89	355.0
90	257.5
91	160.0
92	89.5
93	19.0
94	12.0
95	5.0
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1273648.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.57409097216355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.11306323784069	51.02568490957117
2	7.936520399924618	9.297489358152328
3	1.9364199171391852	3.4027210916046005
4	0.8554646464045582	2.0043225608788124
5	0.4219561169621747	1.2357847990601654
6	0.28337771415736884	0.9959155205122487
7	0.20732216773457932	0.8500595259401978
8	0.139414148713066	0.6532845623620693
9	0.10566633340395011	0.5570378482948144
>10	0.8082079705977223	9.352054514713652
>50	0.09438173368950878	3.9088750319446595
>100	0.08745808630267292	10.05834578288383
>500	0.00792630125832216	3.160695836781655
>1k	0.002552537693357984	2.6211128224707507
>5k	2.6868817824820884E-4	0.8766158348291501
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5844	0.4588394909739582	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5296	0.415813474366544	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4210	0.33054658744017185	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3066	0.24072585204075225	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2679	0.21034069067748704	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2225	0.1746950491815635	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2130	0.1672361594412271	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2097	0.16464517668932074	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1793	0.14077672952024423	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	1539	0.12083401379345	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	1466	0.11510244588771781	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	1462	0.11478838737233521	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	1432	0.11243294850696581	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	1325	0.10403188322048165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.851462884564652E-5	0.0
3	0.0	0.0	0.0	3.925731442282326E-4	0.0
4	0.0	0.0	0.0	3.925731442282326E-4	0.0
5	0.0	0.0	0.0	3.925731442282326E-4	0.0
6	0.0	0.0	0.0	3.925731442282326E-4	0.0
7	0.0	0.0	0.0	5.496024019195256E-4	0.0
8	0.0	0.0	0.0	6.281170307651722E-4	0.0
9	0.0	0.0	0.0	0.0012562340615303444	0.0
10	7.851462884564652E-5	0.0	0.0	0.001648807205758577	7.851462884564652E-5
11	7.851462884564652E-5	0.0	0.0	0.0018843510922955165	7.851462884564652E-5
12	7.851462884564652E-5	0.0	0.0	0.0025909827519063353	7.851462884564652E-5
13	7.851462884564652E-5	0.0	0.0	0.003140585153825861	7.851462884564652E-5
14	1.5702925769129304E-4	0.0	0.0	0.0038472168134366796	7.851462884564652E-5
15	1.5702925769129304E-4	0.0	0.0	0.004867906988430084	7.851462884564652E-5
16	1.5702925769129304E-4	0.0	0.0	0.007929977513410298	7.851462884564652E-5
17	1.5702925769129304E-4	0.0	0.0	0.011698679698001332	7.851462884564652E-5
18	1.5702925769129304E-4	0.0	0.0	0.012483825986457796	7.851462884564652E-5
19	1.5702925769129304E-4	0.0	0.0	0.015781440397974952	7.851462884564652E-5
20	1.5702925769129304E-4	0.0	0.0	0.01782282074796176	7.851462884564652E-5
21	1.5702925769129304E-4	0.0	0.0	0.02276924236523749	7.851462884564652E-5
22	1.5702925769129304E-4	0.0	0.0	0.03085624913633908	7.851462884564652E-5
23	1.5702925769129304E-4	0.0	0.0	0.042554928834340415	7.851462884564652E-5
24	1.5702925769129304E-4	0.0	0.0	0.05967111792269136	7.851462884564652E-5
25	1.5702925769129304E-4	0.0	0.0	0.06438199565343014	7.851462884564652E-5
26	1.5702925769129304E-4	0.0	0.0	0.0720764292803035	7.851462884564652E-5
27	1.5702925769129304E-4	0.0	0.0	0.08652312098790246	7.851462884564652E-5
28	1.5702925769129304E-4	0.0	0.0	0.12633003781264526	7.851462884564652E-5
29	1.5702925769129304E-4	0.0	0.0	0.18553006796226273	7.851462884564652E-5
30	1.5702925769129304E-4	0.0	0.0	0.263887667550218	7.851462884564652E-5
31	1.5702925769129304E-4	0.0	0.0	0.5349201663253897	7.851462884564652E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTAAG	95	2.8194336E-10	23.368422	35
ATTCGCG	45	0.003826031	20.555557	11
ACGTATT	45	0.003826031	20.555557	31
TAACGCT	105	2.2599124E-8	19.38095	32
GTGTTAT	100	2.8755312E-7	18.5	1
TAGCGAT	50	0.007035288	18.5	4
GCTTTAT	670	0.0	18.223883	1
ATTGCCG	95	3.6070069E-6	17.526316	11
GGTATCA	2790	0.0	17.37276	1
CAATACG	75	2.0674881E-4	17.266666	4
ACGTTAA	130	1.3933459E-8	17.076923	20
TTTAGAC	120	1.041426E-7	16.958332	3
ACGCTAA	120	1.041426E-7	16.958332	34
TATTGAG	725	0.0	16.84138	5
CATAATA	100	5.878792E-6	16.650002	2
GAGTATA	125	1.6580088E-7	16.28	1
TACGTTA	125	1.6580088E-7	16.28	19
TATACTG	160	6.2937033E-10	16.1875	5
GCTCGTA	80	3.382924E-4	16.1875	9
GCACCGT	70	0.0025926998	15.857142	6
>>END_MODULE