FastQCFastQC Report
Fri 10 Feb 2017
ERR1630842.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630842.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701809
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT130021.8526408182283214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111831.593453489482181No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT98911.4093578167279133No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53210.7581834943695507No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16430.23410928044524937No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT15180.21629816659518475No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12270.1748338935522343No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11260.1604425135613821No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT10280.14647860030293142Illumina PCR Primer Index 10 (95% over 22bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10220.1456236668381283No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9990.14234642188971644No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9950.14177646624651435No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9030.1286674864528668No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA8110.11555850665921924No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT8000.11399112864041357No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC7370.10501432725998099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCC250.005494903329.630
GCCGTCT1300.028.46153836
ATACACT556.255905E-726.909094
TGCCGTC1600.024.2812535
TACCGTC851.9736035E-923.9411777
ATGCCGT1650.023.54545634
CGTTATA551.9012896E-523.5454542
CCTACAC400.001930442323.1253
CGGACTT400.001930442323.12531
ATACCGT903.8216967E-922.6111136
GCGGTTA502.7006498E-422.213
CGGTTAC502.7006498E-422.214
AGGACTA502.7006498E-422.25
CAAGCGG603.723625E-521.58333410
AAGACGG2100.021.1428575
TTTCGAG806.9521775E-720.812512
CGCAAGA2150.020.6511632
AGGACTC1001.2876626E-820.355
ACGCCCT759.257343E-619.73333431
TCTTTCG851.2438941E-619.58823610
Produced by FastQC (version 0.11.5)