##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630842.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 701809 Sequences flagged as poor quality 0 Sequence length 43 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.983244728978967 33.0 31.0 34.0 30.0 34.0 2 32.08938186885606 33.0 31.0 34.0 30.0 34.0 3 32.152614172802004 34.0 31.0 34.0 30.0 34.0 4 35.78803492118226 37.0 35.0 37.0 35.0 37.0 5 35.77426194306428 37.0 35.0 37.0 35.0 37.0 6 35.88143355243378 37.0 35.0 37.0 35.0 37.0 7 35.80543139230189 37.0 35.0 37.0 35.0 37.0 8 35.848445944694355 37.0 35.0 37.0 35.0 37.0 9 37.48945938282353 39.0 37.0 39.0 35.0 39.0 10 37.41519558740341 39.0 37.0 39.0 34.0 39.0 11 37.53270476725149 39.0 37.0 39.0 35.0 39.0 12 37.481742183414575 39.0 37.0 39.0 35.0 39.0 13 37.5254620559155 39.0 37.0 39.0 35.0 39.0 14 38.68462501905789 40.0 38.0 41.0 35.0 41.0 15 38.65512411496575 40.0 38.0 41.0 34.0 41.0 16 38.589798648920144 40.0 38.0 41.0 34.0 41.0 17 38.66746222975197 40.0 38.0 41.0 35.0 41.0 18 38.706608208216196 40.0 38.0 41.0 35.0 41.0 19 38.80324988707754 40.0 38.0 41.0 35.0 41.0 20 38.7911041323209 40.0 38.0 41.0 35.0 41.0 21 38.748363158637176 40.0 38.0 41.0 35.0 41.0 22 38.629231030095085 40.0 38.0 41.0 34.0 41.0 23 38.53518977385585 40.0 38.0 41.0 34.0 41.0 24 38.44419635541864 40.0 38.0 41.0 34.0 41.0 25 38.37694443929901 40.0 38.0 41.0 34.0 41.0 26 38.16756980887963 40.0 37.0 41.0 34.0 41.0 27 37.97276894425691 40.0 37.0 41.0 33.0 41.0 28 37.80337527731904 40.0 37.0 41.0 33.0 41.0 29 37.64735561954891 40.0 36.0 41.0 33.0 41.0 30 37.442949577449134 39.0 36.0 41.0 33.0 41.0 31 37.266683670343355 39.0 35.0 41.0 32.0 41.0 32 37.0372722492872 39.0 35.0 41.0 32.0 41.0 33 36.8204910452844 39.0 35.0 41.0 31.0 41.0 34 36.65983052368949 39.0 35.0 41.0 31.0 41.0 35 36.472420558870006 39.0 35.0 41.0 31.0 41.0 36 36.30775039932517 39.0 35.0 41.0 30.0 41.0 37 36.0403870568773 39.0 35.0 41.0 30.0 41.0 38 35.745816881801176 38.0 35.0 41.0 28.0 41.0 39 35.517492651134425 38.0 35.0 40.0 27.0 41.0 40 35.144185953728154 38.0 35.0 40.0 25.0 41.0 41 34.876729993488254 38.0 34.0 40.0 23.0 41.0 42 34.5268157005681 38.0 34.0 40.0 21.0 41.0 43 33.661242588795524 37.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 7.0 12 5.0 13 7.0 14 12.0 15 6.0 16 10.0 17 17.0 18 38.0 19 64.0 20 135.0 21 294.0 22 586.0 23 879.0 24 1534.0 25 2498.0 26 3833.0 27 5803.0 28 8083.0 29 11166.0 30 13961.0 31 17611.0 32 21367.0 33 27120.0 34 34924.0 35 44974.0 36 62930.0 37 105499.0 38 148515.0 39 189927.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.4356983167785 18.753820483920837 12.755892272683877 21.054588926616784 2 18.846153298119575 22.47221110017113 33.51438924265719 25.167246359052108 3 21.87176283005775 26.101403658260296 29.290305481975864 22.736528029706086 4 13.155146200746925 15.879676664163611 32.430048631465255 38.53512850362421 5 13.021206624594441 40.17246857763295 31.804379824140188 15.001944973632428 6 32.84754114011077 33.338415437818554 16.7026926129474 17.11135080912328 7 27.12988861641843 32.858797764064015 20.961116201131645 19.050197418385913 8 31.59876832585504 29.321938020173583 19.551758384403733 19.527535269567643 9 28.047802179795355 11.639776634383429 21.103035156288964 39.20938602953225 10 19.262220917657082 27.881232643069552 28.647822983176336 24.20872345609703 11 33.87118147530169 22.977619266780565 19.618015727925975 23.533183529991778 12 22.44713305187024 29.235732229139266 26.69187770461764 21.625257014372856 13 30.687979208018135 19.301690345948828 27.20013564944308 22.810194796589954 14 23.689066398407544 21.97463982365572 27.545956235955938 26.790337541980797 15 27.523870454781857 25.76669720678988 25.646436566074243 21.062995772354018 16 24.07962850291176 25.190472051512593 27.143425062944477 23.586474382631174 17 21.59647425439115 27.303155131951858 27.43310501860193 23.667265595055063 18 20.99217878368616 22.895260676337863 31.582667078934584 24.529893461041393 19 21.754779434290526 25.505515033292536 31.74681430417678 20.99289122824016 20 22.8505191583465 22.845959513200885 32.39570880396233 21.907812524490282 21 25.21198787704347 23.650879370313007 30.553327187311645 20.58380556533188 22 24.342663032249515 24.267571376257642 29.992063367668408 21.39770222382443 23 22.481900346105565 24.532885728168203 30.832890430302264 22.152323495423968 24 21.249656245502692 26.47344220436045 30.4293618349152 21.84753971522166 25 21.49359726079318 25.672369547839942 30.842437187325896 21.991596004040986 26 21.791826551098662 26.20228580710706 30.59849617203541 21.407391469758867 27 21.901543012415058 26.127336640025987 30.290292657973893 21.68082768958506 28 20.665166733398973 25.848058374856976 32.259774383058634 21.22700050868541 29 20.662316955182963 26.83750137145577 31.718031544195075 20.782150129166197 30 20.197090661419274 27.558780237927984 31.8639401888548 20.38018891179794 31 20.908252815224653 27.694002214277674 30.00032772449484 21.39741724600283 32 19.61716079446117 27.49708253955136 30.544492874842017 22.341263791145455 33 19.661047378987732 27.056649316266963 31.59962325931984 21.682680045425464 34 20.847837517045235 27.23590036605401 30.817074161203408 21.09918795569735 35 20.32846543717735 27.391640745558977 31.121430474673307 21.158463342590363 36 19.623002839803995 28.002490706160792 30.0721421355383 22.302364318496913 37 20.338012194200985 27.572031706632433 30.371226359308583 21.718729739857995 38 19.675011292246182 26.300318177737818 31.330319217906865 22.694351312109134 39 20.213191908339734 26.782643140797568 30.802682781212553 22.20148216965015 40 19.39644547163117 27.209112450823515 31.798680267708168 21.59576180983715 41 18.677731405553363 27.537406901307904 31.70634745350943 22.078514239629303 42 19.24711709311223 27.474426802734076 31.76092070634603 21.517535397807666 43 18.688845540595803 26.44665428912995 31.233854225294916 23.63064594497933 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28.0 1 73.0 2 118.0 3 637.5 4 1157.0 5 1157.0 6 1933.5 7 2710.0 8 2929.0 9 3148.0 10 4274.0 11 5400.0 12 5400.0 13 9391.5 14 13383.0 15 20657.0 16 27931.0 17 25061.0 18 22191.0 19 22191.0 20 22452.0 21 22713.0 22 16549.0 23 10385.0 24 9864.0 25 9343.0 26 9343.0 27 10185.0 28 11027.0 29 11902.5 30 12778.0 31 14481.5 32 16185.0 33 16185.0 34 17764.5 35 19344.0 36 21883.0 37 24422.0 38 27260.5 39 30099.0 40 30099.0 41 33495.0 42 36891.0 43 38753.5 44 40616.0 45 42070.0 46 43524.0 47 43524.0 48 45603.0 49 47682.0 50 44108.5 51 40535.0 52 40146.0 53 39757.0 54 39757.0 55 40519.0 56 41281.0 57 39356.0 58 37431.0 59 36131.0 60 34831.0 61 34831.0 62 32765.5 63 30700.0 64 26453.5 65 22207.0 66 19958.0 67 17709.0 68 17709.0 69 15254.0 70 12799.0 71 11210.0 72 9621.0 73 7366.0 74 5111.0 75 5111.0 76 3936.5 77 2762.0 78 2442.0 79 2122.0 80 1776.0 81 1430.0 82 1430.0 83 1231.0 84 1032.0 85 858.5 86 685.0 87 556.0 88 427.0 89 427.0 90 336.5 91 246.0 92 140.0 93 34.0 94 22.5 95 11.0 96 11.0 97 6.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 701809.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.918026845937476 #Duplication Level Percentage of deduplicated Percentage of total 1 79.64226184646557 30.995196846119295 2 10.568996908340576 8.226490108268575 3 3.2261119639198235 3.766617360594953 4 1.5313082720891678 2.383819857702894 5 0.893977488625036 1.7395919950986456 6 0.5935593059754906 1.3860094202766093 7 0.4083207866112237 1.1123727534562937 8 0.324431923914697 1.0101000259673059 9 0.24886948888460958 0.8716958504591376 >10 2.0351307991913927 16.049317505022156 >50 0.2996412692612992 8.058816340828105 >100 0.2138199015136311 15.243238232864117 >500 0.009902465421729055 2.453756844590212 >1k 0.0022005478714953457 1.0796473022209505 >5k 7.335159571651152E-4 2.171284341801309 >10k+ 7.335159571651152E-4 3.452045214729467 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 13002 1.8526408182283214 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 11183 1.593453489482181 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 9891 1.4093578167279133 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5321 0.7581834943695507 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1643 0.23410928044524937 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT 1518 0.21629816659518475 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1227 0.1748338935522343 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 1126 0.1604425135613821 No Hit TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT 1028 0.14647860030293142 Illumina PCR Primer Index 10 (95% over 22bp) AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1022 0.1456236668381283 No Hit GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA 999 0.14234642188971644 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 995 0.14177646624651435 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 903 0.1286674864528668 No Hit GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 811 0.11555850665921924 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT 800 0.11399112864041357 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC 737 0.10501432725998099 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.4248891080051695E-4 0.0 2 0.0 0.0 0.0 0.002707289305209822 0.0 3 0.0 0.0 0.0 0.004987111878018093 0.0 4 0.0 0.0 0.0 0.008121867915629466 0.0 5 0.0 0.0 0.0 0.009689245934435153 0.0 6 0.0 0.0 0.0 0.009689245934435153 0.0 7 0.0 0.0 0.0 0.01068666831003877 0.0 8 0.0 0.0 0.0 0.011256623953240839 0.0 9 0.0 0.0 0.0 0.01923600295806979 0.0 10 0.0 2.849778216010339E-4 0.0 0.029352715624906493 0.0 11 0.0 2.849778216010339E-4 0.0 0.0327724494841189 0.0 12 0.0 5.699556432020678E-4 0.0 0.0353372498785282 0.0 13 0.0 5.699556432020678E-4 0.0 0.036762138986533376 0.0 14 0.0 5.699556432020678E-4 0.0 0.040751828488947844 0.0 15 0.0 5.699556432020678E-4 0.0 0.04730631838577163 0.0 16 0.0 5.699556432020678E-4 0.0 0.06226765401982591 0.0 17 0.0 5.699556432020678E-4 0.0 0.07751396747548123 0.0 18 0.0 5.699556432020678E-4 0.0 0.08577832430191121 0.0 19 0.0 5.699556432020678E-4 0.0 0.10159459340076858 0.0 20 0.0 5.699556432020678E-4 0.0 0.10957397240559753 0.0 21 0.0 5.699556432020678E-4 0.0 0.13422455397408697 0.0 22 0.0 5.699556432020678E-4 0.0 0.17796864958984568 0.0 23 0.0 5.699556432020678E-4 0.0 0.24978306063330621 0.0 24 0.0 8.549334648031017E-4 0.0 0.3466755199776577 0.0 25 0.0 8.549334648031017E-4 0.0 0.3721810350109503 0.0 26 0.0 8.549334648031017E-4 0.0 0.4088006850866831 0.0 27 0.0 8.549334648031017E-4 0.0 0.4439954460544108 0.0 28 0.0 8.549334648031017E-4 0.0 0.5004210547314155 0.0 29 0.0 8.549334648031017E-4 0.0 0.5793599113149019 0.0 30 0.0 8.549334648031017E-4 0.0 0.7261234894394344 0.0 31 0.0 8.549334648031017E-4 0.0 1.203461340621166 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCCC 25 0.0054949033 29.6 30 GCCGTCT 130 0.0 28.461538 36 ATACACT 55 6.255905E-7 26.90909 4 TGCCGTC 160 0.0 24.28125 35 TACCGTC 85 1.9736035E-9 23.941177 7 ATGCCGT 165 0.0 23.545456 34 CGTTATA 55 1.9012896E-5 23.545454 2 CCTACAC 40 0.0019304423 23.125 3 CGGACTT 40 0.0019304423 23.125 31 ATACCGT 90 3.8216967E-9 22.611113 6 GCGGTTA 50 2.7006498E-4 22.2 13 CGGTTAC 50 2.7006498E-4 22.2 14 AGGACTA 50 2.7006498E-4 22.2 5 CAAGCGG 60 3.723625E-5 21.583334 10 AAGACGG 210 0.0 21.142857 5 TTTCGAG 80 6.9521775E-7 20.8125 12 CGCAAGA 215 0.0 20.651163 2 AGGACTC 100 1.2876626E-8 20.35 5 ACGCCCT 75 9.257343E-6 19.733334 31 TCTTTCG 85 1.2438941E-6 19.588236 10 >>END_MODULE