FastQCFastQC Report
Fri 10 Feb 2017
ERR1630841.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630841.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551905
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73240.4719361043362835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70690.4555046861760224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58760.37863142395958516No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38280.24666458320580192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATACG358.8708015E-426.428576
TAATACT3350.016.5671634
CGAACGA1059.347232E-615.85714416
GGTATCA35000.015.698571
TTGCGCG1302.5904774E-715.65384618
ACGTTAA855.365653E-415.23529420
TACGTTA855.365653E-415.23529419
CGAATTA1101.4524414E-515.13636415
ATGCGTT1101.4524414E-515.13636436
ACGAACG1101.4524414E-515.13636415
CGCGTAA1252.9601779E-614.79999910
ATTTGCG1651.6460035E-814.57575716
GTACACG908.2763145E-414.3888893
GTCGTAA908.2763145E-414.38888920
CTCGTAT2252.7284841E-1113.977777536
GTTAGAC800.006299931613.8750013
TCGCGTA1356.570044E-613.7037049
TCTCGTA2304.0017767E-1113.67391235
GCATTCG1907.1286195E-913.63157922
CGCAATA1501.3062254E-613.56666736
Produced by FastQC (version 0.11.5)