##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630841.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1551905
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.095885379581865	33.0	31.0	34.0	30.0	34.0
2	32.267953257448106	34.0	31.0	34.0	30.0	34.0
3	32.36724735083655	34.0	31.0	34.0	30.0	34.0
4	35.94012197911599	37.0	35.0	37.0	35.0	37.0
5	35.89506703052055	37.0	35.0	37.0	35.0	37.0
6	35.97143768465209	37.0	35.0	37.0	35.0	37.0
7	35.92020581156707	37.0	35.0	37.0	35.0	37.0
8	35.922004246393946	37.0	35.0	37.0	35.0	37.0
9	37.63455623894504	39.0	37.0	39.0	35.0	39.0
10	37.551326917562605	39.0	37.0	39.0	35.0	39.0
11	37.6470679584124	39.0	37.0	39.0	35.0	39.0
12	37.592924180281656	39.0	37.0	39.0	35.0	39.0
13	37.62745722193046	39.0	37.0	39.0	35.0	39.0
14	38.93787699633676	40.0	38.0	41.0	36.0	41.0
15	38.947398842068296	40.0	38.0	41.0	36.0	41.0
16	38.90646656850774	40.0	38.0	41.0	35.0	41.0
17	38.89950158031581	40.0	38.0	41.0	35.0	41.0
18	38.88954800712673	40.0	38.0	41.0	35.0	41.0
19	38.961510530605935	40.0	38.0	41.0	35.0	41.0
20	38.92503858161421	40.0	38.0	41.0	35.0	41.0
21	38.89073557981964	40.0	38.0	41.0	35.0	41.0
22	38.822722396022954	40.0	38.0	41.0	35.0	41.0
23	38.76742519677429	40.0	38.0	41.0	35.0	41.0
24	38.71538270706003	40.0	38.0	41.0	35.0	41.0
25	38.66580750754718	40.0	38.0	41.0	34.0	41.0
26	38.52850077807598	40.0	38.0	41.0	34.0	41.0
27	38.39791546518634	40.0	38.0	41.0	34.0	41.0
28	38.31439360012372	40.0	38.0	41.0	34.0	41.0
29	38.23363092457335	40.0	38.0	41.0	34.0	41.0
30	38.142015136235784	40.0	38.0	41.0	33.0	41.0
31	38.08643054826165	40.0	37.0	41.0	33.0	41.0
32	37.97965210499354	40.0	37.0	41.0	33.0	41.0
33	37.895703667428094	40.0	37.0	41.0	33.0	41.0
34	37.8260834264984	40.0	37.0	41.0	33.0	41.0
35	37.76659138284882	40.0	37.0	41.0	33.0	41.0
36	37.716002590364745	40.0	37.0	41.0	33.0	41.0
37	37.575939893228	40.0	37.0	41.0	33.0	41.0
38	37.4227410827338	40.0	37.0	41.0	32.0	41.0
39	37.354373495800324	40.0	36.0	41.0	32.0	41.0
40	37.17098340426766	40.0	36.0	41.0	31.0	41.0
41	37.09431118528518	40.0	36.0	41.0	31.0	41.0
42	36.95599215158144	40.0	36.0	41.0	31.0	41.0
43	36.177822740438366	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	5.0
11	12.0
12	6.0
13	4.0
14	6.0
15	6.0
16	6.0
17	21.0
18	24.0
19	92.0
20	164.0
21	341.0
22	644.0
23	1196.0
24	1992.0
25	3306.0
26	4980.0
27	7375.0
28	11106.0
29	15316.0
30	20693.0
31	27520.0
32	35913.0
33	46675.0
34	61675.0
35	82849.0
36	116587.0
37	184900.0
38	337580.0
39	590906.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67586933478531	19.199886590996226	12.285159207554585	24.839084866663875
2	18.160647720060183	21.357815072443223	35.35487030456117	25.126666902935423
3	20.386170545233117	23.934003692236317	29.687641962620138	25.992183799910435
4	14.359061927115386	15.663394344370307	34.3460456664551	35.631498062059215
5	13.9127717224959	37.718417042280294	34.21304783475792	14.15576340046588
6	34.300166569474285	34.589166218293	16.076048469461725	15.03461874277098
7	28.291100292865867	31.07123180864808	21.809582416449462	18.828085482036595
8	27.08980253301587	33.65379968490339	20.43939545268557	18.817002329395162
9	26.737719125848553	13.956975459193702	20.341322439195697	38.96398297576204
10	16.92893572738022	27.379962046645893	33.10769666957707	22.583405556396816
11	34.42105025758664	22.265151539559444	21.92157380767508	21.39222439517883
12	21.270696337726857	26.2211926632107	29.504125574696904	23.00398542436554
13	29.607997912243338	20.320187124856226	26.065448593825007	24.006366369075426
14	22.72748654073542	21.385007458575107	26.21687538863526	29.670630612054218
15	25.37275155373557	27.763232929850734	23.44280094464545	23.421214571768246
16	24.48790357657202	26.8157522528763	25.014095579304147	23.682248591247532
17	22.85842239054581	27.109520234808187	26.443693396180823	23.588363978465175
18	22.46941662021838	25.67231885972402	28.12201777815008	23.736246741907525
19	23.451499930730296	25.92864898302409	28.201532954658955	22.418318131586663
20	23.940640696434382	25.148382149680554	28.123177642961394	22.78779951092367
21	24.010683643650868	25.696353836091774	27.099661383912032	23.193301136345333
22	23.906553558368586	25.569799697790778	27.216678855986675	23.30696788785396
23	23.311478473231286	25.98122952113692	27.34303968348578	23.364252322146008
24	23.437581552994548	26.24716074759731	27.309145856221868	23.00611184318628
25	23.602153482333005	25.754991445997018	27.396393464806156	23.24646160686382
26	23.33950853950467	26.247676243069	27.309790225561485	23.10302499186484
27	23.548477516342817	25.95081528830695	26.954291660894192	23.546415534456038
28	22.874918245640036	26.136264784249036	27.710330207068086	23.278486763042842
29	22.692819470263967	26.519020171982177	27.841394930746404	22.946765427007453
30	22.677612353848982	27.047918525940702	27.502263347305412	22.772205772904915
31	23.040650039789806	26.551689697500812	27.22782644556207	23.179833817147312
32	22.391576803992514	26.470563597642897	27.574046091738865	23.563813506625728
33	22.534304612717918	26.26037031905948	27.76355511452054	23.441769953702064
34	23.077056907478227	26.112358681749203	27.512508819805337	23.29807559096723
35	22.985620898186422	26.209980636701346	27.802410585699512	23.001987879412724
36	22.528054230123622	26.3993607856151	27.55033330004092	23.52225168422036
37	22.99335333026184	25.522631862130734	27.727405994567967	23.75660881303946
38	22.424890698850767	25.62540877179982	28.45779864102506	23.49190188832435
39	22.41677164517158	25.515865984064746	28.48331566687394	23.584046703889737
40	22.036851482532757	25.403294660433467	29.05435577564348	23.505498081390293
41	21.505053466545952	25.57089512566813	29.305273196490766	23.61877821129515
42	21.929306239750503	25.407998556612682	28.992303008238263	23.67039219539856
43	21.341125906547116	25.199094016708496	29.15468408182202	24.305095994922368
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	272.0
1	254.0
2	236.0
3	723.0
4	1210.0
5	1210.0
6	1736.5
7	2263.0
8	2276.0
9	2289.0
10	3364.0
11	4439.0
12	4439.0
13	7492.0
14	10545.0
15	14655.5
16	18766.0
17	18412.0
18	18058.0
19	18058.0
20	20323.5
21	22589.0
22	21495.0
23	20401.0
24	22574.0
25	24747.0
26	24747.0
27	28226.0
28	31705.0
29	37376.0
30	43047.0
31	47418.0
32	51789.0
33	51789.0
34	56208.5
35	60628.0
36	65484.5
37	70341.0
38	76839.5
39	83338.0
40	83338.0
41	87767.0
42	92196.0
43	91810.0
44	91424.0
45	94421.0
46	97418.0
47	97418.0
48	99752.5
49	102087.0
50	103990.5
51	105894.0
52	109083.0
53	112272.0
54	112272.0
55	104082.0
56	95892.0
57	92234.0
58	88576.0
59	81285.5
60	73995.0
61	73995.0
62	68709.5
63	63424.0
64	56254.5
65	49085.0
66	43321.5
67	37558.0
68	37558.0
69	32146.0
70	26734.0
71	23073.0
72	19412.0
73	15680.0
74	11948.0
75	11948.0
76	9466.0
77	6984.0
78	5832.5
79	4681.0
80	3651.5
81	2622.0
82	2622.0
83	1960.5
84	1299.0
85	1063.5
86	828.0
87	696.5
88	565.0
89	565.0
90	432.0
91	299.0
92	166.5
93	34.0
94	21.0
95	8.0
96	8.0
97	7.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1551905.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.78196469605762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.80544946769079	52.15423800274538
2	8.661241580510005	10.528945599411708
3	2.262471813129596	4.125524456144058
4	0.9399443472686153	2.285266565277596
5	0.5334021803014395	1.6210616245941132
6	0.3316457559196824	1.2094848376744485
7	0.2212638562249422	0.9414196328304605
8	0.15996239524397257	0.7778262928332766
9	0.12078991920641258	0.6607663744359441
>10	0.7741691933913255	9.217668780652904
>50	0.1009241704442412	4.352113189906904
>100	0.0853347660786806	9.359300790175448
>500	0.00276295060455515	1.0763506159772163
>1k	3.188019928332865E-4	0.3808307268243919
>5k	3.188019928332865E-4	1.3092025105162142
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7324	0.4719361043362835	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7069	0.4555046861760224	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5876	0.37863142395958516	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3828	0.24666458320580192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.443693396180823E-5	0.0
2	0.0	0.0	0.0	9.665540094271235E-4	0.0
3	0.0	0.0	0.0	0.0015464864150833975	0.0
4	0.0	0.0	0.0	0.003092972830166795	0.0
5	0.0	0.0	0.0	0.003866216037708494	0.0
6	6.443693396180823E-5	0.0	0.0	0.003930652971670302	0.0
7	6.443693396180823E-5	0.0	0.0	0.00438171150940296	0.0
8	6.443693396180823E-5	0.0	0.0	0.004446148443364768	0.0
9	6.443693396180823E-5	0.0	0.0	0.007796869009378796	0.0
10	6.443693396180823E-5	0.0	0.0	0.012049706650858139	0.0
11	6.443693396180823E-5	0.0	0.0	0.013918377735750578	0.0
12	6.443693396180823E-5	0.0	0.0	0.015207116414986743	0.0
13	6.443693396180823E-5	0.0	0.0	0.015915922688566633	0.0
14	6.443693396180823E-5	0.0	0.0	0.017397972169688223	0.0
15	6.443693396180823E-5	0.0	0.0	0.019846575660236936	0.0
16	6.443693396180823E-5	0.0	0.0	0.023132859292289153	0.0
17	6.443693396180823E-5	6.443693396180823E-5	0.0	0.02654801679226499	0.0
18	6.443693396180823E-5	6.443693396180823E-5	0.0	0.028674435613004663	0.0
19	6.443693396180823E-5	6.443693396180823E-5	0.0	0.03183184537713327	0.0
20	6.443693396180823E-5	6.443693396180823E-5	0.0	0.0352470028771091	0.0
21	6.443693396180823E-5	6.443693396180823E-5	0.0	0.04033752066009195	0.0
22	6.443693396180823E-5	6.443693396180823E-5	0.0	0.04832770047135617	0.0
23	6.443693396180823E-5	6.443693396180823E-5	0.0	0.06192389353729771	0.0
24	6.443693396180823E-5	6.443693396180823E-5	0.0	0.0829303340088472	0.0
25	6.443693396180823E-5	6.443693396180823E-5	0.0	0.09027614448049333	0.0
26	6.443693396180823E-5	6.443693396180823E-5	0.0	0.10239028806531328	0.0
27	6.443693396180823E-5	6.443693396180823E-5	0.0	0.11721078287652917	0.0
28	6.443693396180823E-5	6.443693396180823E-5	0.0	0.15400427216872167	0.0
29	6.443693396180823E-5	6.443693396180823E-5	0.0	0.2029763419796959	0.0
30	6.443693396180823E-5	6.443693396180823E-5	0.0	0.28829084254513004	0.0
31	6.443693396180823E-5	6.443693396180823E-5	0.0	0.5783859192411906	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATACG	35	8.8708015E-4	26.42857	6
TAATACT	335	0.0	16.567163	4
CGAACGA	105	9.347232E-6	15.857144	16
GGTATCA	3500	0.0	15.69857	1
TTGCGCG	130	2.5904774E-7	15.653846	18
ACGTTAA	85	5.365653E-4	15.235294	20
TACGTTA	85	5.365653E-4	15.235294	19
CGAATTA	110	1.4524414E-5	15.136364	15
ATGCGTT	110	1.4524414E-5	15.136364	36
ACGAACG	110	1.4524414E-5	15.136364	15
CGCGTAA	125	2.9601779E-6	14.799999	10
ATTTGCG	165	1.6460035E-8	14.575757	16
GTACACG	90	8.2763145E-4	14.388889	3
GTCGTAA	90	8.2763145E-4	14.388889	20
CTCGTAT	225	2.7284841E-11	13.9777775	36
GTTAGAC	80	0.0062999316	13.875001	3
TCGCGTA	135	6.570044E-6	13.703704	9
TCTCGTA	230	4.0017767E-11	13.673912	35
GCATTCG	190	7.1286195E-9	13.631579	22
CGCAATA	150	1.3062254E-6	13.566667	36
>>END_MODULE