FastQCFastQC Report
Fri 10 Feb 2017
ERR1630839.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630839.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045767
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT87670.42854342649969424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT77040.3765824749348288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64200.31381872911235736No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG52470.256480821129679No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG48770.23839469499703533No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT47160.23052478605823634No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG45990.22480565968656255No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA40210.19655219778205438No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39970.19537904365453154No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC39390.1925439211796847No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC35620.1741156250931802No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC34350.16790768450170523No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC31860.15573621042865587No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT30170.1474752501140159No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA29750.14542223039085098No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG29610.1447378904831293No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT29110.14229381938412342No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA27270.1332996377397817No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG26320.12865590265167048No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA26240.12826485127582957No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA25740.12582078017682366No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG25590.1250875588471219No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC25260.123474471921778No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA24790.12117704508871245No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG23840.11653331000060123No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG23830.11648442857862112No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG23030.11257391482021167No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC22980.11232950771031108No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT22830.11159628638060934No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC22770.11130299784872862No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG21700.106072685696856No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA21530.105241701523194No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG21410.10465512445943256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG1552.1827873E-1117.90322722
CGATCGC1552.1827873E-1117.90322719
GTAAACG1900.017.52631627
CGAATTA852.7247974E-517.41176415
TATACAG2700.017.129635
CTTATAC17200.016.45639437
GGTATCA39550.016.3249031
CGCACCG700.0025934215.8571435
GTATTAG3750.015.7866681
CGCAAGA2700.015.7592582
TTATGCG855.3666916E-415.23529420
TCTTATA26250.014.87047637
GGCCGAT2006.184564E-1114.816
GTTACCG750.00410586214.79999916
TGTCGTG750.00410586214.79999911
TACCGTT750.00410586214.79999918
GCCTAAA2151.2732926E-1114.6279071
CGCACGC2059.276846E-1114.43902423
CGGACCA2950.014.4237299
AGGACCG3850.014.4155845
Produced by FastQC (version 0.11.5)