##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630839.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2045767
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07978621221283	33.0	31.0	34.0	30.0	34.0
2	32.25098997099865	33.0	31.0	34.0	30.0	34.0
3	32.370830109196206	34.0	31.0	34.0	30.0	34.0
4	35.950349673252134	37.0	35.0	37.0	35.0	37.0
5	35.911679580323664	37.0	35.0	37.0	35.0	37.0
6	35.98263047551359	37.0	35.0	37.0	35.0	37.0
7	35.91943852843457	37.0	35.0	37.0	35.0	37.0
8	35.919806116727855	37.0	35.0	37.0	35.0	37.0
9	37.660211549018044	39.0	37.0	39.0	35.0	39.0
10	37.571176971766576	39.0	37.0	39.0	35.0	39.0
11	37.661543567767005	39.0	37.0	39.0	35.0	39.0
12	37.57558803128607	39.0	37.0	39.0	35.0	39.0
13	37.63871741014495	39.0	37.0	39.0	35.0	39.0
14	38.94932756271853	40.0	38.0	41.0	36.0	41.0
15	38.96151467884661	40.0	38.0	41.0	36.0	41.0
16	38.93639647134791	40.0	38.0	41.0	36.0	41.0
17	38.897046926653914	40.0	38.0	41.0	35.0	41.0
18	38.88943266755207	40.0	38.0	41.0	35.0	41.0
19	38.95174621547811	40.0	38.0	41.0	35.0	41.0
20	38.92025435936742	40.0	38.0	41.0	35.0	41.0
21	38.880506431084285	40.0	38.0	41.0	35.0	41.0
22	38.81588128071281	40.0	38.0	41.0	35.0	41.0
23	38.78034497574748	40.0	38.0	41.0	35.0	41.0
24	38.73059444208456	40.0	38.0	41.0	35.0	41.0
25	38.68097930996052	40.0	38.0	41.0	34.0	41.0
26	38.55565858673055	40.0	38.0	41.0	34.0	41.0
27	38.43426646338513	40.0	38.0	41.0	34.0	41.0
28	38.350920217209485	40.0	38.0	41.0	34.0	41.0
29	38.27027760248357	40.0	38.0	41.0	34.0	41.0
30	38.18005325142111	40.0	38.0	41.0	34.0	41.0
31	38.10524903373649	40.0	37.0	41.0	33.0	41.0
32	38.02252260399156	40.0	37.0	41.0	33.0	41.0
33	37.94299888501477	40.0	37.0	41.0	33.0	41.0
34	37.855035299718885	40.0	37.0	41.0	33.0	41.0
35	37.800400534371704	40.0	37.0	41.0	33.0	41.0
36	37.746391451225875	40.0	37.0	41.0	33.0	41.0
37	37.59879106467159	40.0	37.0	41.0	33.0	41.0
38	37.47911272398078	40.0	36.0	41.0	32.0	41.0
39	37.397704137372436	40.0	36.0	41.0	32.0	41.0
40	37.22016094697001	40.0	36.0	41.0	31.0	41.0
41	37.146080174330706	40.0	36.0	41.0	31.0	41.0
42	37.01385592787448	39.0	36.0	41.0	31.0	41.0
43	36.22176425761096	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	9.0
10	12.0
11	4.0
12	5.0
13	5.0
14	5.0
15	4.0
16	4.0
17	19.0
18	41.0
19	91.0
20	185.0
21	369.0
22	741.0
23	1383.0
24	2412.0
25	3890.0
26	6273.0
27	9301.0
28	13959.0
29	19999.0
30	27480.0
31	35882.0
32	47171.0
33	60309.0
34	79193.0
35	108176.0
36	155717.0
37	249935.0
38	447165.0
39	776026.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.659451931720476	18.50088499814495	11.20411073206284	24.635552338071737
2	18.66722847714329	20.706316995043913	35.02911133086026	25.597343196952536
3	20.507320726162853	22.32140805868899	28.66861182138533	28.50265939376283
4	13.253659874267207	15.299542909823064	34.203748520725966	37.24304869518376
5	14.952338169498287	36.656031698624524	33.5199463086461	14.871683823231091
6	36.10274288323157	34.17050915377949	15.71219987417922	14.014548088809722
7	30.92752009393054	29.66916564789636	20.350118073074793	19.053196185098304
8	27.83938737891461	33.07981798513712	19.825229363852287	19.255565272095993
9	26.664620164466434	12.600261906658968	19.57842706427467	41.15669086459992
10	16.676630329846947	26.6428190502633	33.160814501358175	23.519736118531583
11	36.44862782516288	21.560519844146476	20.760086559222042	21.230765771468597
12	21.937835540411005	24.921997470875226	28.976906949813934	24.163260038899836
13	30.683112984029954	19.39546390180309	25.260843488041406	24.660579626125557
14	23.24854198938589	20.42373349457685	25.346337095084632	30.981387420952633
15	25.110630878296504	27.576845261459393	23.250888297640934	24.061635562603172
16	26.478675235254062	25.494154515152506	23.76761380939276	24.25955644020067
17	23.939432007652876	26.005258663376623	25.01848939786398	25.036819931106525
18	23.211098820149118	24.354093110310217	27.525715294068192	24.909092775472473
19	25.575542082749404	25.337587320550192	26.302946523235537	22.783924073464867
20	25.4155043071865	24.70672368847479	26.3851650750061	23.49260692933262
21	24.70418185453182	25.015507631123192	25.91722322239043	24.363087291954557
22	25.824250757784245	23.91596892510242	25.993331596413473	24.266448720699866
23	23.150388094049813	24.846866725291786	26.700841298153698	25.301903882504707
24	24.14400075863967	25.648913097141563	25.86335589536834	24.34373024885043
25	24.21854492715935	25.215188239911974	26.29209484755595	24.27417198537272
26	25.314906340751413	25.33802725334801	25.147194181937632	24.19987222396294
27	25.109164435637098	24.870085400732343	24.958267485984475	25.062482677646088
28	24.093115198358365	25.327371103356345	26.34762414292537	24.23188955535992
29	24.39378482495807	25.888187657734242	26.720491629789706	22.997535887517984
30	24.412701935264376	25.13033009135449	25.57348906302624	24.883478910354896
31	23.45990525802792	24.82418574549301	26.371429395429686	25.344479601049386
32	23.004868100815003	25.590988612095124	26.13679856992512	25.267344717164757
33	23.789023872220053	24.46730248361617	26.591493557184176	25.1521800869796
34	24.923268387846708	24.892375329155275	26.684270496102442	23.500085786895575
35	24.799109576017212	24.800136085878794	25.77610255713383	24.62465178097017
36	23.362973398241344	25.02406187996971	25.372342011578052	26.240622710210886
37	23.47672046718908	24.419007638699814	27.682624658624373	24.42164723548674
38	23.14139391240547	23.69688239178753	26.862687686329874	26.299036009477128
39	22.90099507910725	24.71708654993457	28.075777935610457	24.30614043534772
40	23.943195877145346	23.96152641038789	27.48445937391697	24.610818338549795
41	22.153695899875206	24.73761674716622	28.239432936399893	24.86925441655868
42	22.169044666376962	25.513022744036835	27.768607079887396	24.549325509698807
43	22.795851140428017	23.58597044531464	27.473509935393427	26.14466847886392
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	208.0
1	235.5
2	263.0
3	892.5
4	1522.0
5	1522.0
6	2187.5
7	2853.0
8	2824.5
9	2796.0
10	3813.5
11	4831.0
12	4831.0
13	7890.5
14	10950.0
15	15515.5
16	20081.0
17	19040.0
18	17999.0
19	17999.0
20	20326.5
21	22654.0
22	21424.0
23	20194.0
24	23031.5
25	25869.0
26	25869.0
27	28614.0
28	31359.0
29	35719.5
30	40080.0
31	45772.5
32	51465.0
33	51465.0
34	65568.5
35	79672.0
36	85407.0
37	91142.0
38	93503.0
39	95864.0
40	95864.0
41	99047.5
42	102231.0
43	110267.5
44	118304.0
45	117101.5
46	115899.0
47	115899.0
48	117359.5
49	118820.0
50	125489.5
51	132159.0
52	144291.5
53	156424.0
54	156424.0
55	155640.5
56	154857.0
57	141068.5
58	127280.0
59	125660.0
60	124040.0
61	124040.0
62	116264.0
63	108488.0
64	88416.0
65	68344.0
66	61654.0
67	54964.0
68	54964.0
69	49697.5
70	44431.0
71	45327.0
72	46223.0
73	40282.0
74	34341.0
75	34341.0
76	20952.5
77	7564.0
78	6241.0
79	4918.0
80	3964.5
81	3011.0
82	3011.0
83	2287.0
84	1563.0
85	1305.5
86	1048.0
87	871.0
88	694.0
89	694.0
90	505.5
91	317.0
92	174.5
93	32.0
94	21.5
95	11.0
96	11.0
97	6.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2045767.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.862822418012236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.68569974374857	44.95765051991161
2	8.461700728578093	8.776953644812206
3	1.9491643218750057	3.0326748926678597
4	0.7743861641254411	1.606474084520137
5	0.43468383095045343	1.127196516628231
6	0.25950290468668524	0.8075131837634346
7	0.18770136103705282	0.6814305648558711
8	0.12805727938742195	0.5313129552162914
9	0.10359925689217436	0.48356548765531826
>10	0.7906267677393111	8.296804265460297
>50	0.10775731066273715	3.930830456762934
>100	0.0934510607444083	9.802306090180121
>500	0.013349468039068765	5.046626423923936
>1k	0.009941093220583123	9.53702306978048
>5k	3.787083131650713E-4	1.3816378438612513
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8767	0.42854342649969424	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7704	0.3765824749348288	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6420	0.31381872911235736	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	5247	0.256480821129679	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	4877	0.23839469499703533	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	4716	0.23052478605823634	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	4599	0.22480565968656255	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	4021	0.19655219778205438	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3997	0.19537904365453154	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	3939	0.1925439211796847	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	3562	0.1741156250931802	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	3435	0.16790768450170523	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	3186	0.15573621042865587	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	3017	0.1474752501140159	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	2975	0.14542223039085098	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	2961	0.1447378904831293	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	2911	0.14229381938412342	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	2727	0.1332996377397817	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	2632	0.12865590265167048	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	2624	0.12826485127582957	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	2574	0.12582078017682366	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	2559	0.1250875588471219	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	2526	0.123474471921778	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	2479	0.12117704508871245	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	2384	0.11653331000060123	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	2383	0.11648442857862112	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	2303	0.11257391482021167	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	2298	0.11232950771031108	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	2283	0.11159628638060934	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	2277	0.11130299784872862	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	2170	0.106072685696856	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	2153	0.105241701523194	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	2141	0.10465512445943256	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.3993279782106174E-4	0.0
3	0.0	4.888142198011797E-5	0.0	7.332213297017695E-4	0.0
4	0.0	4.888142198011797E-5	0.0	0.0015153240813836572	0.0
5	4.888142198011797E-5	4.888142198011797E-5	0.0	0.001710849769304129	0.0
6	4.888142198011797E-5	4.888142198011797E-5	0.0	0.001759731191284247	0.0
7	4.888142198011797E-5	4.888142198011797E-5	0.0	0.0020530197231649547	0.0
8	4.888142198011797E-5	4.888142198011797E-5	0.0	0.0021996639891053086	0.0
9	4.888142198011797E-5	4.888142198011797E-5	0.0	0.0037149880704889656	0.0
10	4.888142198011797E-5	4.888142198011797E-5	0.0	0.006110177747514746	0.0
11	4.888142198011797E-5	4.888142198011797E-5	0.0	0.007087806187117106	0.0
12	4.888142198011797E-5	9.776284396023594E-5	0.0	0.007723264672858639	0.0
13	4.888142198011797E-5	9.776284396023594E-5	0.0	0.008260960314639937	0.0
14	4.888142198011797E-5	9.776284396023594E-5	0.0	0.008945300222361589	0.0
15	9.776284396023594E-5	9.776284396023594E-5	0.0	0.010216217193844655	0.0
16	9.776284396023594E-5	9.776284396023594E-5	0.0	0.012464762604930082	0.0
17	9.776284396023594E-5	9.776284396023594E-5	0.0	0.014957715125916099	0.0
18	9.776284396023594E-5	9.776284396023594E-5	0.0	0.016570802051259992	0.0
19	1.9552568792047188E-4	9.776284396023594E-5	0.0	0.018965991728285773	0.0
20	1.9552568792047188E-4	9.776284396023594E-5	0.0	0.021116774295410964	0.0
21	1.9552568792047188E-4	9.776284396023594E-5	0.0	0.0250272880538204	0.0
22	1.9552568792047188E-4	9.776284396023594E-5	0.0	0.031235228645295383	0.0
23	1.9552568792047188E-4	9.776284396023594E-5	0.0	0.042086904324881574	0.0
24	1.9552568792047188E-4	9.776284396023594E-5	0.0	0.05924428343990298	0.0
25	1.9552568792047188E-4	9.776284396023594E-5	0.0	0.06276374582247148	0.0
26	1.9552568792047188E-4	9.776284396023594E-5	0.0	0.06921609352384704	0.0
27	1.9552568792047188E-4	9.776284396023594E-5	0.0	0.08192526323867771	0.0
28	1.9552568792047188E-4	9.776284396023594E-5	0.0	0.12450098178336047	0.0
29	1.9552568792047188E-4	1.466442659403539E-4	0.0	0.18975768012681796	0.0
30	2.4440710990058986E-4	1.466442659403539E-4	0.0	0.2769132555173683	0.0
31	2.932885318807078E-4	1.466442659403539E-4	0.0	0.6409332050033069	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACG	155	2.1827873E-11	17.903227	22
CGATCGC	155	2.1827873E-11	17.903227	19
GTAAACG	190	0.0	17.526316	27
CGAATTA	85	2.7247974E-5	17.411764	15
TATACAG	270	0.0	17.12963	5
CTTATAC	1720	0.0	16.456394	37
GGTATCA	3955	0.0	16.324903	1
CGCACCG	70	0.00259342	15.857143	5
GTATTAG	375	0.0	15.786668	1
CGCAAGA	270	0.0	15.759258	2
TTATGCG	85	5.3666916E-4	15.235294	20
TCTTATA	2625	0.0	14.870476	37
GGCCGAT	200	6.184564E-11	14.8	16
GTTACCG	75	0.004105862	14.799999	16
TGTCGTG	75	0.004105862	14.799999	11
TACCGTT	75	0.004105862	14.799999	18
GCCTAAA	215	1.2732926E-11	14.627907	1
CGCACGC	205	9.276846E-11	14.439024	23
CGGACCA	295	0.0	14.423729	9
AGGACCG	385	0.0	14.415584	5
>>END_MODULE