FastQCFastQC Report
Fri 10 Feb 2017
ERR1630838.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630838.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130770
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT125871.1131352971868727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT122541.0836863376283419No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT107640.9519177197838642No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68800.6084349602483264No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA19990.17678219266517506No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18760.16590464904445643No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18030.15944887112321693No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC17200.1521087400620816No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13760.12168699204966527No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC13750.1215985567356757No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG13520.1195645445139153No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC13410.11859175606002989No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13380.11832645011806114No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC12230.10815638900925918No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA11850.10479584707765505No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACA250.005496306329.621
CGTGATA400.001931168323.12524
GCCGTCT652.6827838E-622.7692336
GTCTACT701.21917496E-418.51
GCGTTAC902.1521519E-618.514
TACCGTT902.1521519E-618.518
GCAGTCG2250.018.0888889
GACGGAC1351.1514203E-917.8148147
CAGTCGG2400.017.72916810
TTAGAAC1054.7970934E-717.6190473
CGAGCTC1054.7970934E-717.61904710
GTTACCG953.606283E-617.52631616
TTCCTCG1900.017.52631620
CAATACG752.0672283E-417.2666684
TCGGTGA2250.017.26666613
GGTATCA62000.016.888711
CCGTTTG1107.807321E-716.81818220
CGCAAGA1554.0017767E-1016.7096792
AAGACGG1452.9795046E-916.5862065
ACGGACC1452.9795046E-916.5862068
Produced by FastQC (version 0.11.5)