##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630838.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1130770
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.028335558955405	33.0	31.0	34.0	30.0	34.0
2	32.17171484917357	33.0	31.0	34.0	30.0	34.0
3	32.25645710445095	34.0	31.0	34.0	30.0	34.0
4	35.851894726602225	37.0	35.0	37.0	35.0	37.0
5	35.82913943595957	37.0	35.0	37.0	35.0	37.0
6	35.9283576677839	37.0	35.0	37.0	35.0	37.0
7	35.871181584230214	37.0	35.0	37.0	35.0	37.0
8	35.89727884538854	37.0	35.0	37.0	35.0	37.0
9	37.56546954729963	39.0	37.0	39.0	35.0	39.0
10	37.494492248644725	39.0	37.0	39.0	35.0	39.0
11	37.60432537120723	39.0	37.0	39.0	35.0	39.0
12	37.55680642394121	39.0	37.0	39.0	35.0	39.0
13	37.60133625759438	39.0	37.0	39.0	35.0	39.0
14	38.839007048294526	40.0	38.0	41.0	35.0	41.0
15	38.81744209697816	40.0	38.0	41.0	35.0	41.0
16	38.765155601934964	40.0	38.0	41.0	35.0	41.0
17	38.81094387010621	40.0	38.0	41.0	35.0	41.0
18	38.82840630720659	40.0	38.0	41.0	35.0	41.0
19	38.92443733031474	40.0	38.0	41.0	35.0	41.0
20	38.905755370234445	40.0	38.0	41.0	35.0	41.0
21	38.86656083907426	40.0	38.0	41.0	35.0	41.0
22	38.76543771058659	40.0	38.0	41.0	35.0	41.0
23	38.68547449967721	40.0	38.0	41.0	35.0	41.0
24	38.63281127019641	40.0	38.0	41.0	34.0	41.0
25	38.56632825419847	40.0	38.0	41.0	34.0	41.0
26	38.40175367227641	40.0	38.0	41.0	34.0	41.0
27	38.234536643172355	40.0	38.0	41.0	34.0	41.0
28	38.11580515931622	40.0	37.0	41.0	33.0	41.0
29	38.01808502171087	40.0	37.0	41.0	33.0	41.0
30	37.87315280737904	40.0	37.0	41.0	33.0	41.0
31	37.76051982277563	40.0	37.0	41.0	33.0	41.0
32	37.59024204745439	40.0	36.0	41.0	33.0	41.0
33	37.44526030934673	40.0	36.0	41.0	33.0	41.0
34	37.347495069731245	40.0	36.0	41.0	33.0	41.0
35	37.21764284514092	40.0	35.0	41.0	32.0	41.0
36	37.118773932806846	40.0	35.0	41.0	32.0	41.0
37	36.917832981066	39.0	35.0	41.0	31.0	41.0
38	36.69916693934222	39.0	35.0	41.0	31.0	41.0
39	36.54724568214579	39.0	35.0	41.0	30.0	41.0
40	36.28181946815002	39.0	35.0	41.0	30.0	41.0
41	36.12202835236167	39.0	35.0	41.0	29.0	41.0
42	35.87855620506381	39.0	35.0	41.0	28.0	41.0
43	35.09858238191675	38.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	5.0
11	5.0
12	8.0
13	8.0
14	4.0
15	5.0
16	8.0
17	10.0
18	39.0
19	65.0
20	177.0
21	346.0
22	639.0
23	1020.0
24	1852.0
25	3099.0
26	4652.0
27	6893.0
28	10225.0
29	14342.0
30	18405.0
31	23652.0
32	29924.0
33	38326.0
34	49611.0
35	64826.0
36	90713.0
37	149176.0
38	243582.0
39	379148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.6442954800711	19.9558707783192	12.5763860024585	21.8234477391512
2	18.77658586626812	22.124835289227697	34.77762940297319	24.320949441530992
3	21.52913501419387	25.8862544991466	29.127055015608832	23.4575554710507
4	13.278827701477756	15.868390565720702	34.864119140055	35.988662592746536
5	12.622726106989044	40.22692501569727	33.287317491620755	13.863031385692933
6	32.4490391503135	34.86827559981251	16.83826065424445	15.844424595629528
7	27.134961132679503	32.640147863844994	21.86784226677397	18.35704873670154
8	29.296408641898886	31.16389716741689	20.25186377424233	19.287830416441892
9	27.54998806123261	12.83930419094953	21.829549775816478	37.78115797200138
10	18.368103150950237	27.94432112631216	31.768794715105635	21.918781007631967
11	32.93357623566243	23.324725629438348	21.066353016086385	22.675345118812846
12	21.712284549466293	28.749613095501296	28.428150729149166	21.109951625883248
13	29.797483130963858	20.733305623601616	27.316607267614103	22.152603977820423
14	22.841515073799272	22.307188906674213	27.85066812879719	27.00062789072933
15	25.95045853710304	27.091893134766575	25.845662690025378	21.111985638105008
16	23.41112693120617	26.526791478373145	27.106750267516826	22.955331322903863
17	21.629066919002096	27.184661779141646	27.71323965085738	23.473031650998877
18	21.07961831318482	24.415840533441813	30.817849783775657	23.686691369597707
19	21.63233902561971	25.83283956949689	31.17963865330704	21.35518275157636
20	23.151215543390787	24.284248786225316	31.383924228622973	21.180611441760924
21	23.840038204055645	25.361833087188373	29.830911679651916	20.967217029104063
22	24.05069112197883	24.894983064637373	29.851251801869523	21.20307401151428
23	22.478576545186023	25.31328209980809	30.213040671400904	21.995100683604978
24	21.333073923078963	26.511757474994912	29.989653068263216	22.1655155336629
25	21.96733199501225	26.076478859538195	30.304217480124162	21.651971665325398
26	21.64525058146219	26.602226801206257	29.846122553658127	21.906400063673427
27	22.036223104610134	26.147138675415864	30.240986230621616	21.575651989352387
28	21.118706721968216	26.235220248149492	30.746747791328033	21.89932523855426
29	20.89496537757457	26.59294109323735	31.214659037646914	21.29743449154116
30	20.369305871220494	27.840763373630356	30.63549616632914	21.154434588820006
31	21.722366175261104	27.832008277545388	29.34080316952165	21.10482237767185
32	20.552720712434887	27.266376009268022	30.111074754370915	22.069828523926173
33	20.15608832919161	27.13434208548157	30.363646011125162	22.345923574201652
34	21.166019614952646	26.534308480062258	30.694747826702155	21.604924078282938
35	20.315006588430894	27.275838587864904	30.702530134333244	21.70662468937096
36	20.600829523245224	28.076443485412593	29.329571884644977	21.993155106697206
37	21.21121006040132	26.789444360922204	30.09533326848077	21.904012310195707
38	20.63275467159546	26.75017908151083	29.991068033287053	22.625998213606657
39	20.675734234194397	27.300069863898052	30.39875483077903	21.625441071128524
40	20.019455769077705	26.729573653351256	30.830230727734197	22.42073984983684
41	19.712231488277897	27.15645091397897	30.753734181133208	22.37758341660992
42	19.823748419218763	27.22251209352919	30.994366670498863	21.959372816753188
43	19.373877976953757	26.161023019712232	30.488339803850472	23.97675919948354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	302.0
1	326.0
2	350.0
3	1104.0
4	1858.0
5	1858.0
6	2795.0
7	3732.0
8	3706.0
9	3680.0
10	5286.5
11	6893.0
12	6893.0
13	11877.5
14	16862.0
15	23001.0
16	29140.0
17	26908.5
18	24677.0
19	24677.0
20	25563.0
21	26449.0
22	21123.0
23	15797.0
24	16681.0
25	17565.0
26	17565.0
27	19474.5
28	21384.0
29	24349.5
30	27315.0
31	31192.5
32	35070.0
33	35070.0
34	39689.0
35	44308.0
36	47432.5
37	50557.0
38	55500.0
39	60443.0
40	60443.0
41	64065.5
42	67688.0
43	69602.0
44	71516.0
45	72150.5
46	72785.0
47	72785.0
48	74923.0
49	77061.0
50	74683.5
51	72306.0
52	73483.0
53	74660.0
54	74660.0
55	70191.0
56	65722.0
57	60923.5
58	56125.0
59	53062.0
60	49999.0
61	49999.0
62	46433.5
63	42868.0
64	37113.5
65	31359.0
66	28383.5
67	25408.0
68	25408.0
69	20364.0
70	15320.0
71	12884.5
72	10449.0
73	7422.5
74	4396.0
75	4396.0
76	3324.0
77	2252.0
78	1886.5
79	1521.0
80	1233.0
81	945.0
82	945.0
83	846.5
84	748.0
85	667.0
86	586.0
87	481.5
88	377.0
89	377.0
90	310.0
91	243.0
92	138.5
93	34.0
94	25.5
95	17.0
96	17.0
97	10.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1130770.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.656157820558434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.97122399315626	44.74258180789658
2	9.17018376137187	9.657332867646387
3	2.1580245054500784	3.4089983681883567
4	0.8647843603885583	1.8214488704548244
5	0.47167731303593774	1.241835751779864
6	0.3238843363188559	1.0232702837287502
7	0.2482166287440034	0.9149093783781844
8	0.18999689264463263	0.8003605091609179
9	0.14738236427355095	0.6984530129839618
>10	1.192695430582596	12.764516958598653
>50	0.15972439345152564	5.871195778954554
>100	0.09262420673176197	9.159730029348399
>500	0.0065559778977191275	2.3850650491240497
>1k	0.0023534279632837895	1.7496968204132526
>5k	1.6810199737741355E-4	0.6089904449053057
>10k+	5.043059921322405E-4	3.151614068437995
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12587	1.1131352971868727	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12254	1.0836863376283419	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10764	0.9519177197838642	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6880	0.6084349602483264	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1999	0.17678219266517506	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1876	0.16590464904445643	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1803	0.15944887112321693	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1720	0.1521087400620816	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1376	0.12168699204966527	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1375	0.1215985567356757	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1352	0.1195645445139153	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1341	0.11859175606002989	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1338	0.11832645011806114	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1223	0.10815638900925918	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1185	0.10479584707765505	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7687062797916463E-4
2	0.0	0.0	0.0	0.0017687062797916463	1.7687062797916463E-4
3	0.0	0.0	0.0	0.002829930047666634	1.7687062797916463E-4
4	0.0	0.0	0.0	0.00495237758341661	1.7687062797916463E-4
5	0.0	0.0	0.0	0.0051292482113957745	1.7687062797916463E-4
6	0.0	0.0	0.0	0.005394554153364522	1.7687062797916463E-4
7	0.0	0.0	0.0	0.007074825119166585	1.7687062797916463E-4
8	0.0	0.0	0.0	0.007428566375124915	1.7687062797916463E-4
9	0.0	0.0	0.0	0.01255781458652069	2.6530594196874695E-4
10	0.0	0.0	0.0	0.01874828656579145	2.6530594196874695E-4
11	0.0	0.0	0.0	0.021843522555426834	2.6530594196874695E-4
12	0.0	0.0	0.0	0.02405440540516639	2.6530594196874695E-4
13	0.0	0.0	0.0	0.025204064487030962	2.6530594196874695E-4
14	0.0	0.0	0.0	0.02776868859272885	2.6530594196874695E-4
15	0.0	0.0	0.0	0.03183671303624964	2.6530594196874695E-4
16	0.0	0.0	0.0	0.0425373860289891	4.421765699479116E-4
17	0.0	0.0	0.0	0.05580268312742644	4.421765699479116E-4
18	0.0	0.0	0.0	0.061904719792707626	4.421765699479116E-4
19	0.0	0.0	0.0	0.07092512181964503	6.190471979270763E-4
20	0.0	0.0	0.0	0.07640811128699912	6.190471979270763E-4
21	0.0	0.0	0.0	0.09161898529320728	7.074825119166585E-4
22	0.0	0.0	0.0	0.12027202702583195	7.074825119166585E-4
23	0.0	0.0	0.0	0.16670056687036267	7.074825119166585E-4
24	0.0	0.0	0.0	0.2260406625573724	7.074825119166585E-4
25	0.0	0.0	0.0	0.24169371313352847	7.074825119166585E-4
26	0.0	0.0	0.0	0.26804743670242404	7.074825119166585E-4
27	8.843531398958232E-5	0.0	0.0	0.2945780308992987	7.074825119166585E-4
28	8.843531398958232E-5	0.0	0.0	0.33348956905471494	7.074825119166585E-4
29	8.843531398958232E-5	0.0	0.0	0.3936255825676309	7.074825119166585E-4
30	8.843531398958232E-5	0.0	0.0	0.49727177056342137	7.959178259062409E-4
31	8.843531398958232E-5	0.0	0.0	0.8511898971497298	7.959178259062409E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGACA	25	0.0054963063	29.6	21
CGTGATA	40	0.0019311683	23.125	24
GCCGTCT	65	2.6827838E-6	22.76923	36
GTCTACT	70	1.21917496E-4	18.5	1
GCGTTAC	90	2.1521519E-6	18.5	14
TACCGTT	90	2.1521519E-6	18.5	18
GCAGTCG	225	0.0	18.088888	9
GACGGAC	135	1.1514203E-9	17.814814	7
CAGTCGG	240	0.0	17.729168	10
TTAGAAC	105	4.7970934E-7	17.619047	3
CGAGCTC	105	4.7970934E-7	17.619047	10
GTTACCG	95	3.606283E-6	17.526316	16
TTCCTCG	190	0.0	17.526316	20
CAATACG	75	2.0672283E-4	17.266668	4
TCGGTGA	225	0.0	17.266666	13
GGTATCA	6200	0.0	16.88871	1
CCGTTTG	110	7.807321E-7	16.818182	20
CGCAAGA	155	4.0017767E-10	16.709679	2
AAGACGG	145	2.9795046E-9	16.586206	5
ACGGACC	145	2.9795046E-9	16.586206	8
>>END_MODULE