FastQCFastQC Report
Fri 10 Feb 2017
ERR1630837.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630837.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657005
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56060.3383212482762575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51560.3111638166450916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38770.23397636096451124No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25010.15093497002121298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG752.0679604E-417.2666661
GGTATCA26750.016.667291
CGAAATA700.002593142215.85714224
ACGAACG1659.749783E-1015.69697115
ACACCGT1302.5907866E-715.6538466
GTCCTAT2500.015.5400011
CGAACGA1557.214112E-915.51612916
GACGGAC2700.015.0740757
AAGACGG3200.015.031255
GTATAGG1601.0972144E-815.031251
ATACCGT1853.0559022E-1015.06
TACCGTC1853.0559022E-1015.07
TCTTATA12500.014.837
TCAATCG1051.6563275E-414.09523834
CTAGACT1203.302088E-513.8750014
TAGGACA2405.456968E-1213.8750014
CGGTTCG800.006300169513.87524
CCTACAC3100.013.7258063
CGAGCCG3100.013.72580615
CAAGACG3650.013.6849324
Produced by FastQC (version 0.11.5)