##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630837.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1657005
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87786639147136	33.0	31.0	34.0	30.0	34.0
2	32.058770492545285	33.0	31.0	34.0	30.0	34.0
3	32.158342310373236	34.0	31.0	34.0	30.0	34.0
4	35.78507367207703	37.0	35.0	37.0	35.0	37.0
5	35.718600728422665	37.0	35.0	37.0	35.0	37.0
6	35.78418170132257	37.0	35.0	37.0	35.0	37.0
7	35.731140219854495	37.0	35.0	37.0	33.0	37.0
8	35.7286019052447	37.0	35.0	37.0	33.0	37.0
9	37.414061514600135	39.0	37.0	39.0	35.0	39.0
10	37.28019529210835	39.0	37.0	39.0	34.0	39.0
11	37.38472122896431	39.0	37.0	39.0	34.0	39.0
12	37.31713724460699	39.0	37.0	39.0	34.0	39.0
13	37.37149495626145	39.0	37.0	39.0	34.0	39.0
14	38.62786352485357	40.0	38.0	41.0	34.0	41.0
15	38.62705544038793	40.0	38.0	41.0	34.0	41.0
16	38.58402479171759	40.0	38.0	41.0	34.0	41.0
17	38.55015102549479	40.0	38.0	41.0	34.0	41.0
18	38.533418426619114	40.0	38.0	41.0	34.0	41.0
19	38.58224024671018	40.0	38.0	41.0	34.0	41.0
20	38.54443348088871	40.0	38.0	41.0	34.0	41.0
21	38.50211013243774	40.0	38.0	41.0	34.0	41.0
22	38.43350683914653	40.0	38.0	41.0	34.0	41.0
23	38.368624113988794	40.0	38.0	41.0	34.0	41.0
24	38.31120606153874	40.0	38.0	41.0	34.0	41.0
25	38.256686008792975	40.0	38.0	41.0	34.0	41.0
26	38.10783914351496	40.0	38.0	41.0	33.0	41.0
27	37.970685664798836	40.0	37.0	41.0	33.0	41.0
28	37.872475339543335	40.0	37.0	41.0	33.0	41.0
29	37.78838446474211	40.0	37.0	41.0	33.0	41.0
30	37.69413007202754	40.0	37.0	41.0	33.0	41.0
31	37.64011394051315	40.0	37.0	41.0	33.0	41.0
32	37.54454090361828	40.0	37.0	41.0	32.0	41.0
33	37.457219501449906	40.0	36.0	41.0	32.0	41.0
34	37.381041095229044	40.0	36.0	41.0	32.0	41.0
35	37.33434358979001	40.0	36.0	41.0	32.0	41.0
36	37.30305762505243	39.0	36.0	41.0	32.0	41.0
37	37.16101218765182	39.0	36.0	41.0	31.0	41.0
38	37.014519569946984	39.0	36.0	41.0	31.0	41.0
39	36.9541268734856	39.0	36.0	41.0	31.0	41.0
40	36.7748347168536	39.0	35.0	41.0	30.0	41.0
41	36.712280892332856	39.0	35.0	41.0	30.0	41.0
42	36.565338668259905	39.0	35.0	40.0	30.0	41.0
43	35.71903343683332	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	5.0
10	6.0
11	5.0
12	2.0
13	3.0
14	1.0
15	5.0
16	9.0
17	29.0
18	69.0
19	124.0
20	283.0
21	518.0
22	944.0
23	1699.0
24	2719.0
25	4480.0
26	6776.0
27	9961.0
28	14179.0
29	19912.0
30	26125.0
31	34300.0
32	43711.0
33	56509.0
34	74840.0
35	101450.0
36	143019.0
37	224202.0
38	423340.0
39	467778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7465185681395	18.46294971952408	11.901171088801783	25.88936062353463
2	19.557695963500414	20.349063521232587	33.717701515686436	26.375538999580566
3	20.80114423311939	22.386051943114232	28.820432044562327	27.992371779204046
4	15.087401667466303	15.161028482110797	33.26067211625795	36.49089773416495
5	15.445034867124722	35.95698262829624	33.279561618703625	15.318420885875419
6	36.27158638628127	33.63194438158002	15.113352102136082	14.983117130002624
7	30.136119082320214	29.70479871816923	20.71611129718981	19.442970902320752
8	28.17197292705816	32.58402961970543	19.281897157823906	19.962100295412508
9	27.188692852465746	13.341359863126545	19.236151972987408	40.23379531142031
10	17.614913654454874	25.9763851044505	32.37147745480551	24.03722378628912
11	37.000552201109834	20.828543064142835	20.866020319793847	21.304884414953484
12	22.186957794333754	24.589907694907378	28.843304636980577	24.37982987377829
13	30.96761928901844	19.076466274996154	24.318635127836068	25.637279308149342
14	23.336320650812763	19.993180467168173	24.82774644614832	31.842752435870743
15	26.455502548272335	26.893461395710936	21.80904704572406	24.841989010292668
16	26.01066381815384	25.308010537083476	23.635836946780486	25.045488697982204
17	24.416220832164054	25.645788636727108	25.026538845688457	24.91145168542038
18	24.608254048720433	24.201133973645224	26.108671971418314	25.081940006216037
19	24.982423106749827	24.60505550677276	26.01295711238047	24.399564274096942
20	25.230642031858686	24.133723193351862	25.916880154254212	24.718754620535243
21	25.6619020461616	24.247301607418205	25.5657043883392	24.525091958080996
22	25.487672034785653	24.32068702267042	25.5888787299978	24.602762212546132
23	24.972405031970332	24.328773902311703	25.735227111565745	24.963593954152223
24	25.099320762459982	24.75864587010902	25.434322769092425	24.70771059833857
25	25.314769720067233	24.30342696612261	25.51549331474558	24.86630999906458
26	25.074094525967034	24.810124290512096	25.554298267054115	24.561482916466755
27	25.13987586036252	24.548688748676074	25.372222775429165	24.93921261553224
28	24.70692605031367	24.597511775764104	25.93118306824663	24.7643791056756
29	24.37192404368122	24.905296000917318	26.028044574397786	24.694735381003678
30	24.30258206824964	25.22207235343285	25.86678978035673	24.60855579796078
31	24.700227217177982	24.93927296538031	25.402759798552204	24.957740018889503
32	24.324126964010368	24.91555547508909	25.662927993578776	25.097389567321766
33	24.334386438182143	24.615254631096466	26.10643902703975	24.943919903681643
34	24.900528362919847	24.502400415206953	25.73836530366535	24.858705918207853
35	24.539093122833062	24.61881527213255	26.248985368179333	24.593106236855046
36	24.3109103472832	24.909098041345683	25.793042266016098	24.986949345355022
37	24.49871907447473	24.301556120832466	26.13426030699968	25.065464497693128
38	24.018636033083787	24.253698691313545	26.625387370587294	25.102277905015374
39	24.12406721766078	24.086771011553978	26.63395704901313	25.15520472177211
40	23.829922058171217	24.127688208544935	27.15731093146973	24.885078801814117
41	23.387436972127425	24.295098687089055	27.405529856578585	24.911934484204938
42	23.717369591522054	24.368182353100927	27.280666020923295	24.633782034453727
43	23.29588625260636	23.845794068213433	27.362983213689763	25.495336465490446
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	111.5
2	119.0
3	410.0
4	701.0
5	701.0
6	1059.5
7	1418.0
8	1403.0
9	1388.0
10	2044.5
11	2701.0
12	2701.0
13	4716.5
14	6732.0
15	9578.5
16	12425.0
17	11981.0
18	11537.0
19	11537.0
20	13451.5
21	15366.0
22	14146.5
23	12927.0
24	14943.0
25	16959.0
26	16959.0
27	20140.5
28	23322.0
29	27477.0
30	31632.0
31	36390.0
32	41148.0
33	41148.0
34	46991.0
35	52834.0
36	59003.0
37	65172.0
38	71854.0
39	78536.0
40	78536.0
41	83693.0
42	88850.0
43	92388.5
44	95927.0
45	99380.5
46	102834.0
47	102834.0
48	107802.0
49	112770.0
50	114468.0
51	116166.0
52	117076.5
53	117987.0
54	117987.0
55	116337.5
56	114688.0
57	111448.5
58	108209.0
59	104437.5
60	100666.0
61	100666.0
62	94057.5
63	87449.0
64	79023.5
65	70598.0
66	62375.0
67	54152.0
68	54152.0
69	46784.0
70	39416.0
71	34148.5
72	28881.0
73	23151.5
74	17422.0
75	17422.0
76	14044.5
77	10667.0
78	8632.5
79	6598.0
80	5387.0
81	4176.0
82	4176.0
83	3151.0
84	2126.0
85	1672.5
86	1219.0
87	998.5
88	778.0
89	778.0
90	553.5
91	329.0
92	191.0
93	53.0
94	35.5
95	18.0
96	18.0
97	11.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1657005.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.88538545700257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.20573593112296	55.47927369512608
2	9.783456614833232	12.891736203402985
3	2.6215406753383923	5.181636536576425
4	1.1229533127967737	2.9594484745533367
5	0.5882478989719401	1.9378469784019092
6	0.36197284184372996	1.430923212590447
7	0.24207751817834697	1.116455941695846
8	0.17152061189693996	0.9040561302920666
9	0.12461892826054342	0.7389509511316011
>10	0.6827086696622682	8.313996845108301
>50	0.053503339146441904	2.4542835829313105
>100	0.03928315139422293	4.7173876682066815
>500	0.00201427477654473	0.8400623395365628
>1k	1.8311588877679364E-4	0.3847420365908515
>5k	1.8311588877679364E-4	0.6491994038555572
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5606	0.3383212482762575	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5156	0.3111638166450916	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3877	0.23397636096451124	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2501	0.15093497002121298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.034984806925749E-5	0.0
2	0.0	0.0	0.0	7.241981768310898E-4	0.0
3	0.0	0.0	0.0	0.0010862972652466347	0.0
4	0.0	0.0	0.0	0.0016897957459392095	0.0
5	0.0	6.034984806925749E-5	0.0	0.0019311951382162396	0.0
6	0.0	6.034984806925749E-5	0.0	0.0020518948343547544	0.0
7	0.0	6.034984806925749E-5	0.0	0.002232944378562527	0.0
8	0.0	6.034984806925749E-5	0.0	0.0022932942266317845	0.0
9	0.0	6.034984806925749E-5	0.0	0.003620990884155449	0.0
10	0.0	6.034984806925749E-5	0.0	0.004586588453263569	0.0
11	0.0	1.2069969613851497E-4	0.0	0.005190086933956144	0.0
12	0.0	1.2069969613851497E-4	0.0	0.005853935262717976	6.034984806925749E-5
13	0.0	1.2069969613851497E-4	0.0	0.006095334654995006	6.034984806925749E-5
14	0.0	1.2069969613851497E-4	0.0	0.006397083895341294	6.034984806925749E-5
15	0.0	1.2069969613851497E-4	0.0	0.007121282072172383	6.034984806925749E-5
16	0.0	1.8104954420777245E-4	0.0	0.008086879641280503	6.034984806925749E-5
17	0.0	1.8104954420777245E-4	0.0	0.009655975691081198	6.034984806925749E-5
18	0.0	1.8104954420777245E-4	0.0	0.010500873564050802	6.034984806925749E-5
19	0.0	1.8104954420777245E-4	0.0	0.012069969613851497	6.034984806925749E-5
20	0.0	1.8104954420777245E-4	0.0	0.013639065663652191	6.034984806925749E-5
21	0.0	1.8104954420777245E-4	0.0	0.016837607611322837	6.034984806925749E-5
22	0.0	1.8104954420777245E-4	0.0	0.01985510001478571	6.034984806925749E-5
23	0.0	3.0174924034628744E-4	0.0	0.025829734973642205	6.034984806925749E-5
24	0.0	3.0174924034628744E-4	0.0	0.034701162639823056	6.034984806925749E-5
25	0.0	3.0174924034628744E-4	0.0	0.0366323577780393	6.034984806925749E-5
26	0.0	3.0174924034628744E-4	6.034984806925749E-5	0.042606992736895784	6.034984806925749E-5
27	0.0	3.0174924034628744E-4	6.034984806925749E-5	0.05292681675673881	6.034984806925749E-5
28	0.0	3.0174924034628744E-4	6.034984806925749E-5	0.07845480249003473	6.034984806925749E-5
29	0.0	4.827987845540599E-4	6.034984806925749E-5	0.11719940495049803	6.034984806925749E-5
30	0.0	5.431486326233174E-4	6.034984806925749E-5	0.17284196487035344	6.034984806925749E-5
31	0.0	5.431486326233174E-4	6.034984806925749E-5	0.37724690028092855	6.034984806925749E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATTAG	75	2.0679604E-4	17.266666	1
GGTATCA	2675	0.0	16.66729	1
CGAAATA	70	0.0025931422	15.857142	24
ACGAACG	165	9.749783E-10	15.696971	15
ACACCGT	130	2.5907866E-7	15.653846	6
GTCCTAT	250	0.0	15.540001	1
CGAACGA	155	7.214112E-9	15.516129	16
GACGGAC	270	0.0	15.074075	7
AAGACGG	320	0.0	15.03125	5
GTATAGG	160	1.0972144E-8	15.03125	1
ATACCGT	185	3.0559022E-10	15.0	6
TACCGTC	185	3.0559022E-10	15.0	7
TCTTATA	1250	0.0	14.8	37
TCAATCG	105	1.6563275E-4	14.095238	34
CTAGACT	120	3.302088E-5	13.875001	4
TAGGACA	240	5.456968E-12	13.875001	4
CGGTTCG	80	0.0063001695	13.875	24
CCTACAC	310	0.0	13.725806	3
CGAGCCG	310	0.0	13.725806	15
CAAGACG	365	0.0	13.684932	4
>>END_MODULE