FastQCFastQC Report
Fri 10 Feb 2017
ERR1630836.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630836.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2960005
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88910.30037111423798274No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC88850.3001684118776827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80040.27040494864028947No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT71670.2421279693784301No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA63860.2157428788127047No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62710.21185775024028675No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA62680.2117563990601367No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG56380.19047265122862966No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50870.17185781780774018No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG46820.15817540848748565No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG45950.1552362242631347No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG45480.15364838910745085No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA42310.14293894773826396No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG40030.1352362580468614No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG37780.1276349195356089No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG36870.12456060040439121No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA36420.12304033270214071No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA33520.11324305195430412No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC32130.1085471139406859No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA31580.10668900897126864No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA30130.10179036859735034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37400.020.1323531
GACGGAC3050.019.4098367
AAGACGG4100.018.9512185
GCTTTAT17600.018.9204541
ACGGACC3250.018.2153848
GAGCGAA3550.017.19718416
ATACCGT1655.4569682E-1116.8181826
GTACTAG904.449767E-516.4444431
AGACGGA4200.016.2976196
GCGTTAT2400.016.18751
CGGACCA3600.015.9305549
TACCGTC1751.3278623E-1015.8571437
ACCGTCG2003.6379788E-1215.7258
CGAACGA1902.7284841E-1115.57894816
CGTTATT2400.015.4166672
TTATTGA21300.015.2863854
TATTGAG21850.015.2402755
CGTCGTA1701.4897523E-915.23529410
CTAGTAC1101.45332615E-515.1363643
TAACGGC1353.9780025E-715.07407536
Produced by FastQC (version 0.11.5)