FastQCFastQC Report
Fri 10 Feb 2017
ERR1630835.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630835.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751665
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT172792.298763411892266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT155942.074594400431043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT126641.6847930926676111No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80661.0730844192559186No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20820.2769850930933328No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20770.276319903148344No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19860.264213446149548No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA19780.2631491422375659No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC17370.23108698688910617No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC16870.22443508743921828No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13530.180000399113967No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT13330.17733963933401184No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA13240.176142297433032No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC12180.16204027059926962No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC11530.15339280131441532No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11400.15166330745744447No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC11260.14980077561147584No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11130.148071281754505No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC11040.14687393985352518No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC10810.14381406610657674No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC10770.1432819141505857No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG10740.14288280018359242No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT9940.13223976106377175TruSeq Adapter, Index 1 (95% over 22bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.12917988731682334No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT9320.12399140574591075No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG9170.12199583591094437No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG9120.12133064596595558No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT8910.11853684819700266No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8730.116142164395043No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.11494482249406318No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT8500.11308229064809457No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC8400.11175191075811698No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG8170.10869203701116853No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC8000.10643039119820664No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8000.10643039119820664No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC7980.10616431522021114No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7970.10603127723121339No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC7890.10496697331923131No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7870.1047008973412358No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG7810.10390266940724924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAC303.598304E-430.83333412
TTAGAAC250.005495148729.5999983
ACACGTA405.9374357E-527.75000211
CCGTCTT1350.027.40740837
ACGTACT358.8665274E-426.42857213
GCCGTCT1450.025.5172436
ACTGTGC451.3226167E-424.6666688
AGCGTCA701.9210893E-723.7857153
TAGGGGT551.9015228E-523.5454544
GTACTAT400.001930568523.1250021
CGAAGTA400.001930568523.12500236
CCGCTTA652.6807957E-622.7692325
TGCATAA652.6807957E-622.7692314
TAGACTA502.7008972E-422.1999995
CCAGGAC502.7008972E-422.1999993
GCTATAT502.7008972E-422.19999919
CTTGCTA502.7008972E-422.19999916
CAGCGTC705.098047E-621.1428592
TTCTGCA705.098047E-621.14285911
AATGCAT450.00382459420.55555727
Produced by FastQC (version 0.11.5)