##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630835.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	751665
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.971261133616704	33.0	31.0	34.0	30.0	34.0
2	32.07901126166577	33.0	31.0	34.0	30.0	34.0
3	32.10143747547112	34.0	31.0	34.0	30.0	34.0
4	35.74037503409099	37.0	35.0	37.0	33.0	37.0
5	35.73894753646904	37.0	35.0	37.0	35.0	37.0
6	35.868219219998274	37.0	35.0	37.0	35.0	37.0
7	35.80288825474114	37.0	35.0	37.0	35.0	37.0
8	35.85152827389861	37.0	35.0	37.0	35.0	37.0
9	37.45260588160949	39.0	37.0	39.0	35.0	39.0
10	37.39714766551589	39.0	37.0	39.0	34.0	39.0
11	37.52555327173675	39.0	37.0	39.0	35.0	39.0
12	37.48643079031218	39.0	37.0	39.0	35.0	39.0
13	37.53666460457784	39.0	37.0	39.0	35.0	39.0
14	38.668444054199675	40.0	38.0	41.0	34.0	41.0
15	38.654351340025144	40.0	38.0	41.0	34.0	41.0
16	38.544548435805844	40.0	38.0	41.0	34.0	41.0
17	38.65768394164954	40.0	38.0	41.0	35.0	41.0
18	38.707758110328406	40.0	38.0	41.0	35.0	41.0
19	38.81182308608223	40.0	38.0	41.0	35.0	41.0
20	38.80044700764303	40.0	38.0	41.0	35.0	41.0
21	38.74741806522852	40.0	38.0	41.0	35.0	41.0
22	38.63103377169351	40.0	38.0	41.0	34.0	41.0
23	38.5156166643385	40.0	38.0	41.0	34.0	41.0
24	38.43931937764829	40.0	38.0	41.0	34.0	41.0
25	38.35489746096998	40.0	38.0	41.0	34.0	41.0
26	38.112339938669486	40.0	37.0	41.0	34.0	41.0
27	37.87898598444786	40.0	37.0	41.0	33.0	41.0
28	37.71435679458269	40.0	36.0	41.0	33.0	41.0
29	37.577701502664084	40.0	36.0	41.0	33.0	41.0
30	37.34802737921814	39.0	35.0	41.0	33.0	41.0
31	37.1478863589498	39.0	35.0	41.0	32.0	41.0
32	36.88302235703405	39.0	35.0	41.0	32.0	41.0
33	36.638320262350916	39.0	35.0	41.0	31.0	41.0
34	36.45752163530296	39.0	35.0	41.0	31.0	41.0
35	36.22271889738115	39.0	35.0	41.0	30.0	41.0
36	36.0486227242189	38.0	35.0	41.0	30.0	41.0
37	35.72350315632629	38.0	35.0	41.0	29.0	41.0
38	35.3903095128814	38.0	35.0	40.0	26.0	41.0
39	35.11333772358697	38.0	35.0	40.0	25.0	41.0
40	34.6956928951062	38.0	34.0	40.0	22.0	41.0
41	34.40489446761523	38.0	34.0	40.0	21.0	41.0
42	33.97905183825241	37.0	33.0	40.0	18.0	41.0
43	33.1168512568764	36.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	9.0
12	4.0
13	2.0
14	6.0
15	2.0
16	9.0
17	22.0
18	37.0
19	102.0
20	209.0
21	348.0
22	703.0
23	1127.0
24	1868.0
25	2914.0
26	4497.0
27	6601.0
28	9309.0
29	12662.0
30	16149.0
31	19662.0
32	24136.0
33	30490.0
34	38829.0
35	48675.0
36	67950.0
37	116731.0
38	156147.0
39	192455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.498154097902656	19.836496311521756	13.043310517318222	19.62203907325737
2	19.978314807793364	22.758276625890524	32.49279931884549	24.770609247470617
3	23.613843933135108	26.008660773083754	28.917802478497734	21.459692815283404
4	13.61311222419562	16.499238357512986	32.142776369792394	37.744873048499
5	12.581535657507	41.356455335821146	30.858294586019035	15.203714420652817
6	32.00122394949878	32.36375246951767	16.818928645074603	18.816094935908946
7	26.344315619325098	34.24544178590197	20.513659675520344	18.89658291925259
8	32.09967206135712	29.04911097363852	19.19591839449755	19.655298570506808
9	28.63775751165745	11.572974662914994	22.311934172803046	37.47733365262451
10	21.705413980962263	28.404275840966385	28.687646757531617	21.202663420539736
11	32.2672999274943	23.41481910159446	18.946605203115748	25.371275767795492
12	22.336679238756627	30.988804853225837	26.248528267246712	20.42598764077082
13	30.92108851682598	19.846607198685582	28.290528360373308	20.94177592411513
14	23.096459193922826	22.739651307430837	28.89106184270919	25.272827655937153
15	27.597001323727994	25.35065487950084	26.984760498360306	20.06758329841086
16	23.131049071062243	24.784179122348387	29.197315293382026	22.887456513207347
17	21.803862092820605	27.47061523418012	27.974563136503626	22.750959536495646
18	20.484790431907832	22.409983170694392	32.73998390240333	24.365242494994447
19	20.989536562165327	25.040277251169073	33.789121483639654	20.18106470302595
20	22.47956203894022	22.15907352344462	35.25347062853798	20.107893809077183
21	23.508876959815876	23.90014168545828	32.862644928259265	19.728336426466576
22	23.98462080847186	23.742890782462933	32.59603679830776	19.67645161075745
23	21.793219053700785	23.852913199364078	32.93288898645008	21.420978760485056
24	20.020487850305653	26.21274104820632	32.871957587489106	20.894813513998923
25	20.492772711247696	25.511630846188126	33.17794496218395	20.817651480380224
26	20.93233022689629	26.681034769478423	32.24840853305662	20.13822647056867
27	21.140401641688783	26.100590023481207	32.80025011141932	19.958758223410694
28	19.755875290189113	26.087552300559423	33.396393340118266	20.76017906913319
29	19.322437522034416	26.46710968317003	34.106017973432316	20.104434821363242
30	17.90398648334032	27.98001769405254	34.30278115916	19.813214663447148
31	20.473349164854024	28.286005068747382	31.403351226942856	19.83729453945574
32	18.80651620070111	27.57677954940033	32.843088343876595	20.773615906021963
33	18.2432333552846	27.531280557163097	32.857190370710356	21.368295716841946
34	19.75853604996907	27.403830163703248	32.895904425508704	19.941729360818982
35	18.556537819374324	28.401482043197433	32.8722236634671	20.16975647396114
36	19.118889398867847	29.08782502843687	31.255945135133338	20.537340437561944
37	19.384300186918374	27.822234639101197	32.19386295756753	20.599602216412897
38	18.878223676770904	27.46755536043317	31.67062454683935	21.983596415956576
39	19.296229038201858	28.30902064084399	31.94268723433977	20.452063086614384
40	18.110594480253837	28.116248594786242	32.0531087652079	21.720048159752018
41	18.49427604052337	28.30689203302003	31.69377315692496	21.50505876953164
42	18.495340344435355	28.868711460557563	32.025835977463366	20.61011221754372
43	17.89094876041854	27.34502737256624	31.330180332994082	23.43384353402114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	406.0
1	345.0
2	284.0
3	932.5
4	1581.0
5	1581.0
6	2506.0
7	3431.0
8	3605.5
9	3780.0
10	5563.0
11	7346.0
12	7346.0
13	13416.0
14	19486.0
15	27175.5
16	34865.0
17	31493.5
18	28122.0
19	28122.0
20	29935.0
21	31748.0
22	22441.5
23	13135.0
24	11693.5
25	10252.0
26	10252.0
27	10906.0
28	11560.0
29	12378.0
30	13196.0
31	14793.0
32	16390.0
33	16390.0
34	18661.5
35	20933.0
36	22168.0
37	23403.0
38	27335.0
39	31267.0
40	31267.0
41	33754.5
42	36242.0
43	38523.5
44	40805.0
45	41780.0
46	42755.0
47	42755.0
48	46556.5
49	50358.0
50	49676.0
51	48994.0
52	48029.0
53	47064.0
54	47064.0
55	44839.0
56	42614.0
57	38927.0
58	35240.0
59	33193.0
60	31146.0
61	31146.0
62	29741.5
63	28337.0
64	25779.0
65	23221.0
66	21934.0
67	20647.0
68	20647.0
69	16413.5
70	12180.0
71	10879.5
72	9579.0
73	6958.0
74	4337.0
75	4337.0
76	3314.0
77	2291.0
78	1778.0
79	1265.0
80	1054.0
81	843.0
82	843.0
83	807.5
84	772.0
85	803.5
86	835.0
87	673.0
88	511.0
89	511.0
90	440.5
91	370.0
92	215.5
93	61.0
94	35.5
95	10.0
96	10.0
97	6.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	751665.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.25858333650938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.55493172371051	27.2543925828705
2	9.861534523292882	6.75684404584187
3	3.190914125083027	3.279485924214054
4	1.5863623353666219	2.173861050722282
5	0.9575635456488284	1.6402385264306898
6	0.6675214066948365	1.3721002644095415
7	0.49677418077809915	1.1913145770128881
8	0.39800279218521767	1.0908009459392565
9	0.3137371207735243	0.9673370367998662
>10	2.409588303568675	16.59506754932913
>50	0.31268683559145877	7.567914827148391
>100	0.21962064155640437	14.427329812815973
>500	0.022195703136019592	5.111710915195539
>1k	0.007009169411374609	3.4208362388005433
>5k	3.893983006319226E-4	1.0760232851914824
>10k+	0.001168194901895768	6.074742417278022
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17279	2.298763411892266	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15594	2.074594400431043	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12664	1.6847930926676111	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8066	1.0730844192559186	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2082	0.2769850930933328	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2077	0.276319903148344	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1986	0.264213446149548	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1978	0.2631491422375659	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1737	0.23108698688910617	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1687	0.22443508743921828	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1353	0.180000399113967	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	1333	0.17733963933401184	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1324	0.176142297433032	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1218	0.16204027059926962	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1153	0.15339280131441532	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1140	0.15166330745744447	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1126	0.14980077561147584	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1113	0.148071281754505	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1104	0.14687393985352518	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1081	0.14381406610657674	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1077	0.1432819141505857	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1074	0.14288280018359242	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	994	0.13223976106377175	TruSeq Adapter, Index 1 (95% over 22bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	971	0.12917988731682334	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	932	0.12399140574591075	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	917	0.12199583591094437	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	912	0.12133064596595558	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	891	0.11853684819700266	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	873	0.116142164395043	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	864	0.11494482249406318	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	850	0.11308229064809457	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	840	0.11175191075811698	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	817	0.10869203701116853	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	800	0.10643039119820664	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	800	0.10643039119820664	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	798	0.10616431522021114	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	797	0.10603127723121339	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	789	0.10496697331923131	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	787	0.1047008973412358	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	781	0.10390266940724924	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.330379889977583E-4	0.0
4	0.0	0.0	0.0	3.9911396699327494E-4	0.0
5	0.0	0.0	0.0	5.321519559910332E-4	0.0
6	0.0	0.0	0.0	5.321519559910332E-4	0.0
7	0.0	0.0	0.0	6.651899449887916E-4	0.0
8	0.0	0.0	0.0	7.982279339865499E-4	0.0
9	0.0	0.0	0.0	0.0025277217909574078	0.0
10	0.0	3.9911396699327494E-4	0.0	0.0034589877139417162	0.0
11	0.0	3.9911396699327494E-4	0.0	0.004257215647928266	0.0
12	0.0	3.9911396699327494E-4	0.0	0.00625278548289464	0.0
13	0.0	3.9911396699327494E-4	0.0	0.006784937438885674	0.0
14	0.0	3.9911396699327494E-4	0.0	0.008913545262849806	0.0
15	0.0	3.9911396699327494E-4	0.0	0.01330379889977583	0.0
16	0.0	3.9911396699327494E-4	0.0	0.02514417992057632	0.0
17	0.0	3.9911396699327494E-4	0.0	0.04350342240226697	0.0
18	0.0	3.9911396699327494E-4	0.0	0.049224055929170576	0.0
19	0.0	3.9911396699327494E-4	0.0	0.0615965889059621	0.0
20	0.0	3.9911396699327494E-4	0.0	0.06838152634484777	0.0
21	0.0	3.9911396699327494E-4	0.0	0.08940152860649359	0.0
22	0.0	3.9911396699327494E-4	0.0	0.12904684932782556	0.0
23	0.0	3.9911396699327494E-4	0.0	0.18505584269588182	0.0
24	0.0	5.321519559910332E-4	0.0	0.2620848383255839	0.0
25	0.0	5.321519559910332E-4	0.0	0.2833709165652252	0.0
26	0.0	5.321519559910332E-4	0.0	0.31609826185867373	0.0
27	0.0	5.321519559910332E-4	0.0	0.34736218927314694	0.0
28	0.0	5.321519559910332E-4	0.0	0.3979166250922951	0.0
29	0.0	5.321519559910332E-4	0.0	0.47720726653495904	0.0
30	0.0	5.321519559910332E-4	0.0	0.6272741181244305	0.0
31	0.0	5.321519559910332E-4	0.0	1.0008447912301357	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGTAC	30	3.598304E-4	30.833334	12
TTAGAAC	25	0.0054951487	29.599998	3
ACACGTA	40	5.9374357E-5	27.750002	11
CCGTCTT	135	0.0	27.407408	37
ACGTACT	35	8.8665274E-4	26.428572	13
GCCGTCT	145	0.0	25.51724	36
ACTGTGC	45	1.3226167E-4	24.666668	8
AGCGTCA	70	1.9210893E-7	23.785715	3
TAGGGGT	55	1.9015228E-5	23.545454	4
GTACTAT	40	0.0019305685	23.125002	1
CGAAGTA	40	0.0019305685	23.125002	36
CCGCTTA	65	2.6807957E-6	22.76923	25
TGCATAA	65	2.6807957E-6	22.76923	14
TAGACTA	50	2.7008972E-4	22.199999	5
CCAGGAC	50	2.7008972E-4	22.199999	3
GCTATAT	50	2.7008972E-4	22.199999	19
CTTGCTA	50	2.7008972E-4	22.199999	16
CAGCGTC	70	5.098047E-6	21.142859	2
TTCTGCA	70	5.098047E-6	21.142859	11
AATGCAT	45	0.003824594	20.555557	27
>>END_MODULE