FastQCFastQC Report
Fri 10 Feb 2017
ERR1630834.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630834.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1642101
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88080.5363860079252128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT83110.5061199037087244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69860.42543059166275404No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54610.33256176081739186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA1005.88088E-616.6510
ATACCGT1251.6588638E-716.286
GGTATCA46050.016.0293161
ATAGACT1059.3478975E-615.8571424
ACCGTCG1059.3478975E-615.8571428
CTTACTC1659.749783E-1015.696973
GGACTAG1101.4525434E-515.1363641
AGTCGGT1853.0559022E-1015.011
TCTAGCG1252.9604307E-614.80000128
TTAGACT1152.2108738E-514.4782614
TACCGTC1152.2108738E-514.4782617
CTAGCGG1304.446467E-614.23076929
GCCGTTC1702.4383553E-814.14705858
AAGACGG2500.014.0600015
ATTCCGT1854.9021764E-914.029
GACGGAC2009.840733E-1013.8749997
CGAGCCG2405.456968E-1213.87499915
ATACCGC2009.840733E-1013.87499927
TCTTGCG1753.5681296E-813.7428582
CTAGCAC2700.013.7037033
Produced by FastQC (version 0.11.5)