FastQCFastQC Report
Fri 10 Feb 2017
ERR1630833.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630833.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1949142
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33270.17069048843029394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31710.16268696688081216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25690.13180158243986329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTA1301.3948011E-817.07692319
GGTATCA19100.016.7565461
CGAATTA2109.094947E-1214.976191515
TTAGGAC6200.014.6209683
TAGGGTG2301.8189894E-1214.4782615
AAGACGG2201.8189894E-1114.2954545
TAGGACA6000.014.1833334
ACGTTAA1702.4392648E-814.147058520
GCTTAGG4750.014.0210521
AATGCGT2009.840733E-1013.87500135
TCTACGC800.006300696613.87536
AACGCTA1601.7851562E-713.87533
CTTATAC12200.013.49590237
TCTTATA15600.013.40064137
TATACCA2501.0913936E-1113.325
AGAACGC850.00940780213.0588235
CGCTAAG1703.7346945E-713.05882335
TAATACT3350.012.7014924
CTTAACG1755.3096846E-712.68571430
ATAATAC2204.089088E-912.6136363
Produced by FastQC (version 0.11.5)