##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630833.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1949142
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.877925774520275	33.0	31.0	34.0	30.0	34.0
2	32.07034633700366	33.0	31.0	34.0	30.0	34.0
3	32.17070280154037	34.0	31.0	34.0	30.0	34.0
4	35.79043445782811	37.0	35.0	37.0	35.0	37.0
5	35.72800955497342	37.0	35.0	37.0	35.0	37.0
6	35.799750351693206	37.0	35.0	37.0	35.0	37.0
7	35.75069030373364	37.0	35.0	37.0	33.0	37.0
8	35.74667879508009	37.0	35.0	37.0	33.0	37.0
9	37.43194184928548	39.0	37.0	39.0	35.0	39.0
10	37.31849141827532	39.0	37.0	39.0	34.0	39.0
11	37.41489127010757	39.0	37.0	39.0	34.0	39.0
12	37.35349964240676	39.0	37.0	39.0	34.0	39.0
13	37.40303784947428	39.0	37.0	39.0	34.0	39.0
14	38.672501541703994	40.0	38.0	41.0	35.0	41.0
15	38.68623989427143	40.0	38.0	41.0	35.0	41.0
16	38.641411451808025	40.0	38.0	41.0	34.0	41.0
17	38.60115732973791	40.0	38.0	41.0	34.0	41.0
18	38.58516311279527	40.0	38.0	41.0	34.0	41.0
19	38.64392743063358	40.0	38.0	41.0	34.0	41.0
20	38.605378674309	40.0	38.0	41.0	34.0	41.0
21	38.56530565756625	40.0	38.0	41.0	34.0	41.0
22	38.50449582431654	40.0	38.0	41.0	34.0	41.0
23	38.45142631988844	40.0	38.0	41.0	34.0	41.0
24	38.401060056168305	40.0	38.0	41.0	34.0	41.0
25	38.34875704284244	40.0	38.0	41.0	34.0	41.0
26	38.21666507622328	40.0	38.0	41.0	34.0	41.0
27	38.08521287828183	40.0	38.0	41.0	33.0	41.0
28	38.00577125730193	40.0	37.0	41.0	33.0	41.0
29	37.93203060628728	40.0	37.0	41.0	33.0	41.0
30	37.85428973363665	40.0	37.0	41.0	33.0	41.0
31	37.82530569860995	40.0	37.0	41.0	33.0	41.0
32	37.74221683181626	40.0	37.0	41.0	33.0	41.0
33	37.682835832381635	40.0	37.0	41.0	33.0	41.0
34	37.628749983325996	40.0	37.0	41.0	32.0	41.0
35	37.59002319995157	40.0	37.0	41.0	32.0	41.0
36	37.5747513521334	40.0	37.0	41.0	32.0	41.0
37	37.464094971018014	40.0	37.0	41.0	32.0	41.0
38	37.33387664931544	40.0	36.0	41.0	31.0	41.0
39	37.29329263850453	40.0	36.0	41.0	31.0	41.0
40	37.142014794201756	39.0	36.0	41.0	31.0	41.0
41	37.114218461251156	39.0	36.0	41.0	31.0	41.0
42	36.99671906921097	39.0	36.0	41.0	31.0	41.0
43	36.19486420178725	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	3.0
11	2.0
12	2.0
13	3.0
14	4.0
15	2.0
16	12.0
17	32.0
18	58.0
19	128.0
20	263.0
21	565.0
22	984.0
23	1677.0
24	2918.0
25	4655.0
26	7013.0
27	10570.0
28	15286.0
29	21258.0
30	28850.0
31	37948.0
32	49019.0
33	63329.0
34	84493.0
35	113483.0
36	160345.0
37	249328.0
38	489388.0
39	607517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76255911575452	19.208759546508155	12.308184832095352	25.72049650564197
2	18.938845912714413	21.23185483664094	34.39739126241187	25.431907988232773
3	20.58972614617098	23.041112448451678	29.58517132153532	26.783990083842017
4	15.156925457457692	15.604096571722327	34.064219025602036	35.174758945217945
5	14.67697068761537	36.54643940769836	34.39292776000928	14.383662144676993
6	35.277727328229545	34.442693246566954	15.409446823268905	14.870132601934593
7	29.024360462193112	30.212524279913932	21.28726383198351	19.47585142590945
8	26.896757650289203	33.434659968334785	20.146197660303866	19.522384721072143
9	26.496017221936626	14.04069072443157	19.663626354570372	39.79966569906143
10	16.97562312032679	26.909173369615964	33.1914760443313	22.923727465725946
11	35.6676424806402	21.493303207257348	21.566053165957126	21.27300114614533
12	21.52613816746035	25.2147355092651	28.74982941212082	24.509296911153726
13	30.050760796288827	19.97063323246844	24.935587042914268	25.043018928328465
14	22.883504639477266	21.089022759757885	24.85534660891818	31.172125991846666
15	25.75533234623234	27.72106906526051	22.214133192963878	24.30946539554327
16	25.413592236994536	26.563534108854046	23.732031837598285	24.29084181655313
17	23.772254663846965	26.58923772613796	25.34699883333282	24.291508776682253
18	23.677443716260797	25.35556670576079	26.53300785679032	24.43398172118809
19	24.511092573039832	25.617425513379732	26.302239652113595	23.56924226146684
20	24.736165964306345	24.96698547360839	26.28223084823989	24.014617713845375
21	24.7351398718	25.51779193101375	25.462485544921815	24.284582652264433
22	24.774131387041066	25.34597274082648	25.521383254785952	24.358512617346502
23	24.325010697014378	25.591978419222407	25.7367087672422	24.346302116521013
24	24.723545026478316	25.649542208828297	25.490138737967783	24.136774026725604
25	24.765358296111827	25.306057742329703	25.689149379573166	24.239434581985304
26	24.48754375001924	25.80253260152416	25.53010504108987	24.17981860736673
27	24.702817957850172	25.438731503399957	25.283586316440772	24.5748642223091
28	24.080185025000745	25.468642099959883	26.06346792588739	24.387704949151985
29	23.914830217603438	25.88225998926707	26.10358814288543	24.099321650244057
30	23.868861273319233	26.33286851342796	25.929562853809525	23.868707359443285
31	24.286121791023948	25.831981456456226	25.69946160926192	24.182435143257905
32	23.776974689376146	25.701000748021436	25.878771274745503	24.643253287856915
33	23.779693834517957	25.538826827393795	26.1037933613867	24.577685976701545
34	24.268113867537615	25.319909991165346	26.016626802972798	24.395349338324245
35	24.246309401777808	25.361569346922902	26.407260220137886	23.984861031161405
36	23.831101069085783	25.617066381002513	26.035866037466743	24.51596651244496
37	24.351022142050194	24.552238882544216	26.50155812147088	24.595180853934707
38	23.72028307840065	24.790805390269156	27.069397714481553	24.419513816848646
39	23.66769583745053	24.786034060114655	27.09515263639078	24.451117466044035
40	23.25233359088255	24.439933057724886	27.930648459681233	24.377084891711327
41	22.809061628142025	24.614522697679288	27.9057657164024	24.670649957776295
42	23.165526164845865	24.38631972426842	27.80597822016046	24.64217589072525
43	22.580704740855207	24.240717197618235	28.0397733977309	25.13880466379566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	85.0
1	89.0
2	93.0
3	267.0
4	441.0
5	441.0
6	675.0
7	909.0
8	921.5
9	934.0
10	1435.5
11	1937.0
12	1937.0
13	3223.0
14	4509.0
15	6851.5
16	9194.0
17	9755.5
18	10317.0
19	10317.0
20	13040.0
21	15763.0
22	17446.0
23	19129.0
24	22521.0
25	25913.0
26	25913.0
27	29739.0
28	33565.0
29	40970.5
30	48376.0
31	54217.5
32	60059.0
33	60059.0
34	67082.0
35	74105.0
36	80645.0
37	87185.0
38	97118.5
39	107052.0
40	107052.0
41	113746.0
42	120440.0
43	119109.0
44	117778.0
45	122573.0
46	127368.0
47	127368.0
48	129238.0
49	131108.0
50	135878.0
51	140648.0
52	144861.0
53	149074.0
54	149074.0
55	139306.5
56	129539.0
57	123153.0
58	116767.0
59	107333.0
60	97899.0
61	97899.0
62	91934.0
63	85969.0
64	77348.5
65	68728.0
66	60568.5
67	52409.0
68	52409.0
69	45570.5
70	38732.0
71	33810.5
72	28889.0
73	23579.5
74	18270.0
75	18270.0
76	14438.5
77	10607.0
78	8890.5
79	7174.0
80	5531.0
81	3888.0
82	3888.0
83	2962.0
84	2036.0
85	1611.5
86	1187.0
87	945.5
88	704.0
89	704.0
90	498.5
91	293.0
92	171.0
93	49.0
94	31.0
95	13.0
96	13.0
97	9.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1949142.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.479468768494705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.35559053272596	49.330701220064746
2	9.432746922734598	11.03244058138345
3	2.5199583940854127	4.420974846144719
4	1.0737910688355194	2.5117892509542123
5	0.6003264927179064	1.7553387190898389
6	0.3674997470072602	1.2894713986524466
7	0.2454991117709658	1.0049660347652372
8	0.1910264739000844	0.8936901371516524
9	0.14150602752669186	0.7447677585570837
>10	0.8765156427596572	9.731500776496626
>50	0.098354053874925	4.0605159831172575
>100	0.09111773348751322	10.349317474561563
>500	0.005012529256401816	1.8572616995025584
>1k	0.0010552693171372244	1.0172641195586352
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3327	0.17069048843029394	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3171	0.16268696688081216	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2569	0.13180158243986329	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	5.1304625317190845E-5	0.0	1.0260925063438169E-4	0.0
2	0.0	1.0260925063438169E-4	0.0	0.001693052635467298	0.0
3	0.0	1.0260925063438169E-4	0.0	0.0031295821443486417	0.0
4	0.0	1.5391387595157255E-4	0.0	0.004925244030450322	0.0
5	0.0	1.5391387595157255E-4	0.0	0.005592204159573802	0.0
6	0.0	2.0521850126876338E-4	0.0	0.005746118035525375	0.0
7	0.0	2.0521850126876338E-4	0.0	0.00666960129123481	0.0
8	0.0	2.0521850126876338E-4	0.0	0.007182647544406719	0.0
9	0.0	2.0521850126876338E-4	0.0	0.011851368448271086	0.0
10	0.0	2.0521850126876338E-4	0.0	0.017751400359748034	0.0
11	0.0	2.565231265859542E-4	0.0	0.020727068628145103	0.0
12	0.0	2.565231265859542E-4	0.0	0.02247142588892959	0.0
13	0.0	2.565231265859542E-4	0.0	0.023856650772493743	0.0
14	0.0	2.565231265859542E-4	0.0	0.025241875656057896	0.0
15	0.0	2.565231265859542E-4	0.0	0.026781014415573623	0.0
16	0.0	2.565231265859542E-4	0.0	0.02852537167635811	0.0
17	0.0	2.565231265859542E-4	0.0	0.030834079815631698	0.0
18	0.0	2.565231265859542E-4	0.0	0.031347126068803605	0.0
19	0.0	2.565231265859542E-4	0.0	0.0330401787042709	0.0
20	0.0	2.565231265859542E-4	0.0	0.03540019146886168	0.0
21	0.0	2.565231265859542E-4	0.0	0.038273250486624374	0.0
22	0.0	2.565231265859542E-4	0.0	0.04196718350946211	0.0
23	0.0	3.078277519031451E-4	0.0	0.04673851366396086	0.0
24	0.0	3.5913237722033593E-4	0.0	0.05566551846915207	0.0
25	0.0	3.5913237722033593E-4	0.0	0.059308146866672615	0.0
26	0.0	3.5913237722033593E-4	0.0	0.06402817239585418	0.0
27	0.0	3.5913237722033593E-4	0.0	0.07439170670992673	0.0
28	0.0	3.5913237722033593E-4	0.0	0.09896662223686115	0.0
29	0.0	5.130462531719084E-4	0.0	0.13472594608294317	0.0
30	0.0	5.130462531719084E-4	0.0	0.18900623966853108	0.0
31	0.0	5.130462531719084E-4	0.0	0.38319424649409844	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTTA	130	1.3948011E-8	17.076923	19
GGTATCA	1910	0.0	16.756546	1
CGAATTA	210	9.094947E-12	14.9761915	15
TTAGGAC	620	0.0	14.620968	3
TAGGGTG	230	1.8189894E-12	14.478261	5
AAGACGG	220	1.8189894E-11	14.295454	5
TAGGACA	600	0.0	14.183333	4
ACGTTAA	170	2.4392648E-8	14.1470585	20
GCTTAGG	475	0.0	14.021052	1
AATGCGT	200	9.840733E-10	13.875001	35
TCTACGC	80	0.0063006966	13.875	36
AACGCTA	160	1.7851562E-7	13.875	33
CTTATAC	1220	0.0	13.495902	37
TCTTATA	1560	0.0	13.400641	37
TATACCA	250	1.0913936E-11	13.32	5
AGAACGC	85	0.009407802	13.058823	5
CGCTAAG	170	3.7346945E-7	13.058823	35
TAATACT	335	0.0	12.701492	4
CTTAACG	175	5.3096846E-7	12.685714	30
ATAATAC	220	4.089088E-9	12.613636	3
>>END_MODULE