FastQCFastQC Report
Fri 10 Feb 2017
ERR1630832.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630832.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134756
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT233852.060795448536954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT220691.9448233805329076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT195841.7258335712699469No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137341.2103042416167No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41520.36589363704620204No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33670.29671576973375774No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30170.2658721346262985No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26360.23229663469503575No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA25650.22603978300180833No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC22800.20092425155716293No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21870.19272865708575238No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC20970.18479743662954856No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA19660.17325310463218524No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG19550.17228373324309368No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT19320.17025686579317492No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC17760.15650941700242166No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG17290.15236755743084857No Hit
CCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC14990.13209888293166108No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC14690.12945514277959314No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14300.12601828058190484No Hit
GGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTC13830.12187642101033173No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC13700.12073080027776896No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC13220.11650081603446028No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC12170.1072477255022225No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC12030.10601398009792413No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC11710.103193990602385No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11670.10284149191544262No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG11550.10178399585461544No Hit
CCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC11490.10125524782420185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGG2300.027.3478266
TAAGATA556.2616346E-726.909094
CTCTAAT2400.024.6666681
TTGGTAA2400.023.89583433
AATACTG2500.023.6800025
CGGTCAC551.9026309E-523.54545430
ATTAGAC400.001931171723.1250023
GTTACAC400.001931171723.1250023
TGCTAGA2650.022.3396216
TCGTCCC502.7020735E-422.19999930
GCCGTCT502.7020735E-422.19999936
TAAACAG2650.021.64150837
TCTAATA3350.021.5373132
TACTGGT2750.021.5272737
TCTATAC1301.8189894E-1221.3461533
TAGAGGT2750.020.85454619
CTAGAGG2850.020.77192918
CAGCGCG450.003825778420.5555559
TCCTATA909.478026E-820.5555557
CAAATGC450.003825778420.55555525
Produced by FastQC (version 0.11.5)