##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630832.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1134756
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.988213325155364	33.0	31.0	34.0	30.0	34.0
2	32.081072054256595	33.0	31.0	34.0	30.0	34.0
3	32.13184772761721	34.0	31.0	34.0	30.0	34.0
4	35.75981620718463	37.0	35.0	37.0	35.0	37.0
5	35.7639527792759	37.0	35.0	37.0	35.0	37.0
6	35.90110296839144	37.0	35.0	37.0	35.0	37.0
7	35.82921438617641	37.0	35.0	37.0	35.0	37.0
8	35.886568566282094	37.0	35.0	37.0	35.0	37.0
9	37.51799153298154	39.0	37.0	39.0	35.0	39.0
10	37.46090613312465	39.0	37.0	39.0	35.0	39.0
11	37.58413526784613	39.0	37.0	39.0	35.0	39.0
12	37.555886023074564	39.0	37.0	39.0	35.0	39.0
13	37.59930240509854	39.0	37.0	39.0	35.0	39.0
14	38.76673575640931	40.0	38.0	41.0	35.0	41.0
15	38.72984148134048	40.0	38.0	41.0	35.0	41.0
16	38.64232222609971	40.0	38.0	41.0	34.0	41.0
17	38.7642374219656	40.0	38.0	41.0	35.0	41.0
18	38.806948806615694	40.0	38.0	41.0	35.0	41.0
19	38.922652975617666	40.0	38.0	41.0	35.0	41.0
20	38.905715413710084	40.0	38.0	41.0	35.0	41.0
21	38.84954827293269	40.0	38.0	41.0	35.0	41.0
22	38.721447606357664	40.0	38.0	41.0	35.0	41.0
23	38.60220611303223	40.0	38.0	41.0	34.0	41.0
24	38.52068374170306	40.0	38.0	41.0	34.0	41.0
25	38.42780474392733	40.0	38.0	41.0	34.0	41.0
26	38.18395937100134	40.0	37.0	41.0	34.0	41.0
27	37.96290127569275	40.0	36.0	41.0	33.0	41.0
28	37.79388344278418	40.0	36.0	41.0	33.0	41.0
29	37.64832439749162	40.0	36.0	41.0	33.0	41.0
30	37.41071032010406	39.0	35.0	41.0	33.0	41.0
31	37.19815449312451	39.0	35.0	41.0	33.0	41.0
32	36.9314539865839	39.0	35.0	41.0	32.0	41.0
33	36.68264014466546	39.0	35.0	41.0	31.0	41.0
34	36.470843952356276	39.0	35.0	41.0	31.0	41.0
35	36.21522336079298	39.0	35.0	41.0	30.0	41.0
36	36.0021017734209	39.0	35.0	41.0	30.0	41.0
37	35.66319984208059	38.0	35.0	41.0	28.0	41.0
38	35.31239403008224	38.0	35.0	41.0	25.0	41.0
39	35.012872370800416	38.0	35.0	41.0	23.0	41.0
40	34.60845062727142	38.0	35.0	40.0	21.0	41.0
41	34.29183630665976	38.0	34.0	40.0	18.0	41.0
42	33.876098474033185	38.0	33.0	40.0	15.0	41.0
43	33.074189517394046	37.0	33.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	10.0
11	20.0
12	17.0
13	10.0
14	3.0
15	5.0
16	13.0
17	21.0
18	56.0
19	106.0
20	252.0
21	528.0
22	903.0
23	1620.0
24	2731.0
25	4290.0
26	6599.0
27	9936.0
28	14256.0
29	19404.0
30	24762.0
31	30186.0
32	36628.0
33	45156.0
34	57536.0
35	72127.0
36	99533.0
37	170917.0
38	229792.0
39	307330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.76863396183849	19.86612099869928	12.962786713619492	18.402458325842737
2	18.451543767999464	23.15370000246749	34.49349463673248	23.901261592800566
3	23.329861221266952	28.45871711627874	28.160415102453744	20.051006560000566
4	12.987109123018516	16.413308235426825	32.85402324376342	37.74555939779125
5	11.298992911251405	43.09252385534864	31.38648308535051	14.22200014804945
6	30.988159569105605	32.84820701542886	18.005280430330398	18.15835298513513
7	25.59960026648901	34.30481971454656	22.345773011995533	17.749807006968897
8	31.279764107878698	29.8035877316357	20.13516562150806	18.781482538977542
9	28.760367867629693	11.59077369936797	23.06266721656462	36.58619121643772
10	20.583455826627045	29.144943935083838	29.972170228665902	20.299430009623215
11	30.479944587206415	24.14783442431677	20.485196817641853	24.887024170834966
12	21.76441455255579	31.325236438494265	28.45880524095048	18.451543767999464
13	29.798564625346767	21.10365576388228	29.688320661005534	19.409458949765412
14	22.595342082350744	23.187275502398755	30.78344595666381	23.43393645858669
15	26.036169890267157	26.12279644258325	29.244172315458123	18.596861351691466
16	20.968648766783343	26.3736873830145	30.986837699029575	21.670826151172587
17	20.065194632149996	27.97570579049593	30.10303536619326	21.856064211160813
18	19.23215211023339	23.856846758245826	34.56848873237947	22.342512399141313
19	19.65849927209021	26.460313935330593	35.420478058719226	18.460708733859967
20	21.548332857460107	23.25583649700905	36.90158941657942	18.29424122895142
21	22.59278646687041	25.29627514637508	33.994532745365525	18.116405641388987
22	22.879103525339367	24.51593117815636	33.94535917853706	18.65960611796721
23	20.874179118682783	25.145670082378945	34.174307075706146	19.80584372323213
24	18.491464244295692	27.388971726080317	34.370208220974376	19.749355808649614
25	19.170641089361943	26.629601429734674	34.915435565002525	19.28432191590086
26	19.365837237256294	27.754600989111317	33.95760850790831	18.921953265724085
27	19.547638435046828	27.26727155441346	34.48847153044355	18.696618480096163
28	18.201357824942104	27.405891663053556	35.063749387533534	19.329001124470814
29	18.1869935034492	27.468724554001035	35.70564949645563	18.63863244609414
30	17.008061644970375	28.91855165339509	35.34892082526993	18.72446587636461
31	18.73468833828594	29.167503851048153	33.48182340520782	18.61598440545809
32	17.243618892519624	28.8527225236086	34.5321813676244	19.37147721624737
33	16.889710210829463	29.1497907920293	34.2687767238067	19.691722273334534
34	18.468551829644433	28.258850360782407	34.49234901599992	18.780248793573243
35	17.03714278664312	29.372922460863833	34.034365096990015	19.55556965550303
36	17.542714028390243	29.74286983280987	32.73302806947044	19.98138806932944
37	17.83378981913292	28.639548942680186	33.369376324073194	20.157284914113696
38	17.380388383053273	28.23373482933776	33.031330083295444	21.354546704313528
39	17.57540828160415	29.175523196176094	33.676843303758695	19.57222521846106
40	16.82291170965388	28.736838580276284	33.48799213222931	20.952257577840523
41	16.762546309515	28.8943173686678	33.377836292559806	20.965300029257392
42	16.643049254641525	29.431877866254947	33.57038870030209	20.35468417880143
43	16.26102880266771	28.133008329543973	32.446799135673224	23.159163732115097
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1248.0
1	1006.0
2	764.0
3	2420.5
4	4077.0
5	4077.0
6	6131.5
7	8186.0
8	8234.0
9	8282.0
10	12132.5
11	15983.0
12	15983.0
13	26063.0
14	36143.0
15	46455.5
16	56768.0
17	51459.0
18	46150.0
19	46150.0
20	45914.5
21	45679.0
22	33616.0
23	21553.0
24	19969.5
25	18386.0
26	18386.0
27	18746.5
28	19107.0
29	20605.0
30	22103.0
31	24437.0
32	26771.0
33	26771.0
34	30504.0
35	34237.0
36	36946.5
37	39656.0
38	46410.0
39	53164.0
40	53164.0
41	57058.5
42	60953.0
43	65279.0
44	69605.0
45	74336.0
46	79067.0
47	79067.0
48	78752.5
49	78438.0
50	74334.0
51	70230.0
52	68095.5
53	65961.0
54	65961.0
55	60757.5
56	55554.0
57	50298.0
58	45042.0
59	42143.0
60	39244.0
61	39244.0
62	36263.0
63	33282.0
64	29886.0
65	26490.0
66	24738.0
67	22986.0
68	22986.0
69	17680.0
70	12374.0
71	10441.0
72	8508.0
73	6002.0
74	3496.0
75	3496.0
76	2692.0
77	1888.0
78	1517.5
79	1147.0
80	924.0
81	701.0
82	701.0
83	649.5
84	598.0
85	533.0
86	468.0
87	376.5
88	285.0
89	285.0
90	216.0
91	147.0
92	88.5
93	30.0
94	17.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1134756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.97023160900196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.2755709577158	31.240224850273435
2	9.013044841316928	6.8445480025424805
3	2.705105756702513	3.0814047632651675
4	1.2909844906818222	1.9607592045927282
5	0.7934850941165151	1.5064406400947397
6	0.5188163639340284	1.1819746500669186
7	0.4275361670978116	1.1363553100150205
8	0.30509425799020984	0.9267599710771889
9	0.26766296514363924	0.9146902301690579
>10	1.9380383938660921	14.828648641795542
>50	0.2484865132613185	6.543561787200344
>100	0.1899078708735073	13.506788034175715
>500	0.017433403075105332	4.371784908016897
>1k	0.007903142727381084	5.003638584484968
>5k	0.0	0.0
>10k+	9.29781497338951E-4	6.952420422229766
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23385	2.060795448536954	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22069	1.9448233805329076	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19584	1.7258335712699469	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13734	1.2103042416167	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4152	0.36589363704620204	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3367	0.29671576973375774	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3017	0.2658721346262985	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2636	0.23229663469503575	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2565	0.22603978300180833	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	2280	0.20092425155716293	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2187	0.19272865708575238	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2097	0.18479743662954856	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1966	0.17325310463218524	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1955	0.17228373324309368	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1932	0.17025686579317492	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1776	0.15650941700242166	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1729	0.15236755743084857	No Hit
CCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC	1499	0.13209888293166108	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1469	0.12945514277959314	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1430	0.12601828058190484	No Hit
GGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTC	1383	0.12187642101033173	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1370	0.12073080027776896	No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC	1322	0.11650081603446028	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1217	0.1072477255022225	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1203	0.10601398009792413	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1171	0.103193990602385	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1167	0.10284149191544262	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1155	0.10178399585461544	No Hit
CCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC	1149	0.10125524782420185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.812467173559778E-5	0.0
2	0.0	8.812467173559778E-5	0.0	0.0013218700760339668	0.0
3	0.0	8.812467173559778E-5	0.0	0.0022912414651255425	0.0
4	0.0	8.812467173559778E-5	0.0	0.0038774855563663024	0.0
5	0.0	8.812467173559778E-5	0.0	0.004053734899837498	0.0
6	0.0	8.812467173559778E-5	0.0	0.004053734899837498	0.0
7	0.0	8.812467173559778E-5	0.0	0.00475873227372228	0.0
8	0.0	8.812467173559778E-5	0.0	0.005375604975871465	0.0
9	0.0	8.812467173559778E-5	0.0	0.008283719143146191	0.0
10	0.0	1.7624934347119556E-4	0.0	0.014893069523316026	0.0
11	8.812467173559778E-5	1.7624934347119556E-4	0.0	0.03110800912266602	0.0
12	8.812467173559778E-5	1.7624934347119556E-4	0.0	0.05296292771309427	0.0
13	8.812467173559778E-5	1.7624934347119556E-4	0.0	0.05921977940632171	0.0
14	8.812467173559778E-5	1.7624934347119556E-4	0.0	0.07587534236434969	0.0
15	8.812467173559778E-5	1.7624934347119556E-4	0.0	0.103193990602385	0.0
16	8.812467173559778E-5	1.7624934347119556E-4	0.0	0.15263193144605536	0.0
17	8.812467173559778E-5	1.7624934347119556E-4	0.0	0.22084042736940804	0.0
18	8.812467173559778E-5	1.7624934347119556E-4	0.0	0.24375284202066347	0.0
19	8.812467173559778E-5	1.7624934347119556E-4	0.0	0.2819989495539129	0.0
20	8.812467173559778E-5	1.7624934347119556E-4	0.0	0.311344465241867	0.0
21	8.812467173559778E-5	1.7624934347119556E-4	0.0	0.3863385608888607	0.0
22	1.7624934347119556E-4	1.7624934347119556E-4	0.0	0.5658485172142734	0.0
23	1.7624934347119556E-4	1.7624934347119556E-4	0.0	0.8326900232296635	0.0
24	1.7624934347119556E-4	1.7624934347119556E-4	0.0	1.1969974161846246	0.0
25	1.7624934347119556E-4	1.7624934347119556E-4	0.0	1.2726965092055031	0.0
26	1.7624934347119556E-4	1.7624934347119556E-4	0.0	1.3777411179143357	0.0
27	1.7624934347119556E-4	1.7624934347119556E-4	0.0	1.457405821163316	0.0
28	1.7624934347119556E-4	1.7624934347119556E-4	0.0	1.5631554272460335	0.0
29	1.7624934347119556E-4	1.7624934347119556E-4	0.0	1.7098830056858039	0.0
30	1.7624934347119556E-4	1.7624934347119556E-4	0.0	1.9852726048595468	0.0
31	1.7624934347119556E-4	1.7624934347119556E-4	0.0	2.673614415786301	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACTGG	230	0.0	27.347826	6
TAAGATA	55	6.2616346E-7	26.90909	4
CTCTAAT	240	0.0	24.666668	1
TTGGTAA	240	0.0	23.895834	33
AATACTG	250	0.0	23.680002	5
CGGTCAC	55	1.9026309E-5	23.545454	30
ATTAGAC	40	0.0019311717	23.125002	3
GTTACAC	40	0.0019311717	23.125002	3
TGCTAGA	265	0.0	22.33962	16
TCGTCCC	50	2.7020735E-4	22.199999	30
GCCGTCT	50	2.7020735E-4	22.199999	36
TAAACAG	265	0.0	21.641508	37
TCTAATA	335	0.0	21.537313	2
TACTGGT	275	0.0	21.527273	7
TCTATAC	130	1.8189894E-12	21.346153	3
TAGAGGT	275	0.0	20.854546	19
CTAGAGG	285	0.0	20.771929	18
CAGCGCG	45	0.0038257784	20.555555	9
TCCTATA	90	9.478026E-8	20.555555	7
CAAATGC	45	0.0038257784	20.555555	25
>>END_MODULE