FastQCFastQC Report
Fri 10 Feb 2017
ERR1630831.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630831.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008290
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16680.16542859693143835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14920.14797330133195807No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT13930.13815469755725038No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG13530.13418758492100485No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG10670.10582272957184936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10430.10344246199010207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT1500.023.43333210
TTATACT802.725028E-823.1254
TAGGACC1950.022.7692324
GGTATCA9400.021.6489371
GCGGTTC1700.020.67647211
CGGTTCT1700.020.67647212
ATAGGAC2250.020.5555553
TCTATAT555.1424914E-420.1818183
GACCGCG1800.019.5277777
GGTTCTA1800.019.52777713
AGGACCG2100.019.380955
GTTTTCG1900.018.528
ATTATAC701.2190086E-418.53
TTTCGGA2050.018.0487830
TTCGGAA1950.018.02564231
TTTTCGG1950.018.02564229
TATACTG1054.79582E-717.6190475
TCTTATA7900.017.09493637
GTATACT650.001579752217.0769234
GCCGTCT1851.8189894E-1217.036
Produced by FastQC (version 0.11.5)