##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630831.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1008290
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03836792986145	33.0	31.0	34.0	30.0	34.0
2	32.22187168374178	33.0	31.0	34.0	30.0	34.0
3	32.32516042011723	34.0	31.0	34.0	30.0	34.0
4	35.9118755516766	37.0	35.0	37.0	35.0	37.0
5	35.86345198306043	37.0	35.0	37.0	35.0	37.0
6	35.939064158129106	37.0	35.0	37.0	35.0	37.0
7	35.88785567644229	37.0	35.0	37.0	35.0	37.0
8	35.87933729383411	37.0	35.0	37.0	35.0	37.0
9	37.60551527834254	39.0	37.0	39.0	35.0	39.0
10	37.50594868539805	39.0	37.0	39.0	35.0	39.0
11	37.602088684802986	39.0	37.0	39.0	35.0	39.0
12	37.535692112388304	39.0	37.0	39.0	35.0	39.0
13	37.57282031955092	39.0	37.0	39.0	35.0	39.0
14	38.880486764720466	40.0	38.0	41.0	35.0	41.0
15	38.903427585317715	40.0	38.0	41.0	36.0	41.0
16	38.86420176734868	40.0	38.0	41.0	35.0	41.0
17	38.82717273800196	40.0	38.0	41.0	35.0	41.0
18	38.81163355780579	40.0	38.0	41.0	35.0	41.0
19	38.87639567981434	40.0	38.0	41.0	35.0	41.0
20	38.846116692618196	40.0	38.0	41.0	35.0	41.0
21	38.80330758016047	40.0	38.0	41.0	35.0	41.0
22	38.73642106933521	40.0	38.0	41.0	35.0	41.0
23	38.69028354937568	40.0	38.0	41.0	34.0	41.0
24	38.63857223616222	40.0	38.0	41.0	34.0	41.0
25	38.592283965922505	40.0	38.0	41.0	34.0	41.0
26	38.47686776621805	40.0	38.0	41.0	34.0	41.0
27	38.35193148796477	40.0	38.0	41.0	34.0	41.0
28	38.27139612611451	40.0	38.0	41.0	34.0	41.0
29	38.2078370310129	40.0	38.0	41.0	33.0	41.0
30	38.143021352983766	40.0	38.0	41.0	33.0	41.0
31	38.126209721409516	40.0	38.0	41.0	33.0	41.0
32	38.0393091273344	40.0	38.0	41.0	33.0	41.0
33	37.98070793124994	40.0	37.0	41.0	33.0	41.0
34	37.93599559650497	40.0	37.0	41.0	33.0	41.0
35	37.89878407997699	40.0	37.0	41.0	33.0	41.0
36	37.88225609695623	40.0	37.0	41.0	33.0	41.0
37	37.7780003768757	40.0	37.0	41.0	33.0	41.0
38	37.655140882087494	40.0	37.0	41.0	33.0	41.0
39	37.62003094347856	40.0	37.0	41.0	33.0	41.0
40	37.477943845520635	40.0	37.0	41.0	32.0	41.0
41	37.460462763689016	40.0	37.0	41.0	32.0	41.0
42	37.36027035872616	40.0	36.0	41.0	32.0	41.0
43	36.55415207926291	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	1.0
12	3.0
13	2.0
14	1.0
15	1.0
16	3.0
17	9.0
18	29.0
19	62.0
20	88.0
21	215.0
22	382.0
23	735.0
24	1203.0
25	1973.0
26	3136.0
27	4616.0
28	6775.0
29	9739.0
30	13183.0
31	17309.0
32	22536.0
33	29500.0
34	39496.0
35	53942.0
36	76460.0
37	117279.0
38	228843.0
39	380764.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.81408126630235	18.105307004929138	11.909768023088596	26.170843705679914
2	19.20627993930318	20.24219222644279	34.3771137272015	26.174414107052534
3	20.173759533467553	21.977407293536583	29.79371014291523	28.05512303008063
4	14.729889218379633	14.97237897827014	33.58577393408642	36.7119578692638
5	14.743179045711056	36.63469835067292	34.20236241557488	14.419760188041138
6	36.56071170000694	33.96284799016156	15.017207351059714	14.45923295877178
7	30.3263941921471	30.025786232135598	20.148865901675116	19.49895367404219
8	27.91339793115076	33.27296710271847	19.486457269238016	19.327177696892758
9	26.97011772406748	13.224965039819894	18.66744686548513	41.1374703706275
10	16.819268266074243	26.665939362683353	32.87804103978022	23.63675133146218
11	36.9268761963324	20.579595156155474	20.87593847008301	21.617590177429115
12	22.347935613761912	25.030794711838855	28.14378799750072	24.47748167689851
13	31.39067133463587	18.902795822630395	24.326136329825747	25.380396512907993
14	23.584385444663738	20.009818603774708	24.12331769629769	32.28247825526386
15	26.732190143708657	26.84396354223487	21.446012555911494	24.97783375814498
16	26.653938846958713	25.422348729036287	22.42301322040286	25.50069920360214
17	24.70707832072122	25.794463894316124	24.091878328655447	25.406579456307217
18	24.452984756369695	24.181535074234596	25.779983933193822	25.585496236201887
19	25.408563012625336	24.658877902190838	25.21665393884696	24.715905146336866
20	26.007001953802973	23.717283717977963	25.303037816501206	24.972676511717857
21	26.23818544268018	24.531831120015074	23.967310991877337	25.26267244542741
22	26.193852958970137	24.21019746303147	24.20712295073838	25.388826627260013
23	25.37781788969443	24.497614773527456	24.599668746094874	25.524898590683236
24	25.918733697646513	24.582907695206735	24.440488351565524	25.057870255581232
25	25.79109184857531	24.306796655724046	24.616330619167105	25.285780876533536
26	25.774033264239453	24.576361959356934	24.350534072538654	25.299070703864963
27	25.79466224994793	24.01551141040772	24.47004334070555	25.719782998938793
28	25.09079729046207	24.066191274335758	25.20346328933144	25.639548145870734
29	24.62416566662369	24.838290571165043	25.076515684971586	25.461028077239682
30	24.7196739033413	25.475805571809694	24.828967856469863	24.975552668379137
31	25.474417082387014	24.850985331601027	24.36937785756082	25.30521972845114
32	24.443761219490423	24.737625088020312	24.9329062075395	25.885707484949766
33	24.876969919368435	24.232413293794444	25.28310307550407	25.607513711333045
34	25.10349205089806	23.998948715151393	25.21199258149937	25.68556665245118
35	24.951452459113945	24.114986759761575	25.79267869362981	25.14088208749467
36	24.51040871177935	24.937964276150712	25.1300717055609	25.421555306509042
37	25.549494689027956	23.83877654246298	25.168949409396106	25.442779359112954
38	24.4320582372135	23.9538228089141	26.319610429539118	25.294508524333274
39	24.73246784159319	23.614436322883297	26.086443384343788	25.56665245117972
40	23.953326919834574	23.283777484652234	27.220442531414573	25.542453064098623
41	23.416576580150554	23.66928165507939	27.406301758422675	25.507840006347383
42	24.090589017048668	23.113191641293675	27.485941544595306	25.31027779706235
43	23.092463477769293	22.951829334814388	27.672495016314752	26.283212171101567
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	21.5
2	22.0
3	90.0
4	158.0
5	158.0
6	233.0
7	308.0
8	315.0
9	322.0
10	491.5
11	661.0
12	661.0
13	1055.5
14	1450.0
15	2463.5
16	3477.0
17	3353.0
18	3229.0
19	3229.0
20	3910.0
21	4591.0
22	4865.0
23	5139.0
24	6431.0
25	7723.0
26	7723.0
27	9417.5
28	11112.0
29	15033.5
30	18955.0
31	21269.0
32	23583.0
33	23583.0
34	26911.0
35	30239.0
36	33962.5
37	37686.0
38	43430.0
39	49174.0
40	49174.0
41	53407.0
42	57640.0
43	57870.0
44	58100.0
45	61936.5
46	65773.0
47	65773.0
48	69502.0
49	73231.0
50	76118.5
51	79006.0
52	82838.5
53	86671.0
54	86671.0
55	81909.0
56	77147.0
57	74008.0
58	70869.0
59	66043.0
60	61217.0
61	61217.0
62	57090.0
63	52963.0
64	46986.5
65	41010.0
66	35821.0
67	30632.0
68	30632.0
69	25729.5
70	20827.0
71	17928.0
72	15029.0
73	11919.5
74	8810.0
75	8810.0
76	6931.5
77	5053.0
78	4033.0
79	3013.0
80	2360.5
81	1708.0
82	1708.0
83	1278.0
84	848.0
85	657.5
86	467.0
87	369.5
88	272.0
89	272.0
90	202.5
91	133.0
92	74.5
93	16.0
94	10.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1008290.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.19614796176685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.91246222361593	57.31553894814104
2	7.848627083001712	10.234005052002203
3	1.7995013718258162	3.5196167308487514
4	0.6797545437323138	1.77269511243421
5	0.3616984007764468	1.1790671227277838
6	0.23950343547738906	0.9368820850041204
7	0.157625399248951	0.7193598196377029
8	0.11407449479024324	0.59497741128068
9	0.09185637491887169	0.538981362939806
>10	0.6209324681868642	7.993408491656451
>50	0.09728274928597186	4.508519044918428
>100	0.07348004278143673	8.887386979372968
>500	0.0022867231128911713	1.0028487217425714
>1k	9.146892451564686E-4	0.7967131172932065
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1668	0.16542859693143835	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1492	0.14797330133195807	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	1393	0.13815469755725038	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1353	0.13418758492100485	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1067	0.10582272957184936	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1043	0.10344246199010207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.950668954368287E-4	0.0
3	0.0	0.0	0.0	7.93422527249105E-4	0.0
4	0.0	0.0	0.0	8.926003431552431E-4	0.0
5	0.0	1.9835563181227624E-4	0.0	9.91778159061381E-4	0.0
6	0.0	1.9835563181227624E-4	0.0	9.91778159061381E-4	0.0
7	0.0	1.9835563181227624E-4	0.0	0.0010909559749675193	0.0
8	0.0	1.9835563181227624E-4	0.0	0.0011901337908736575	0.0
9	0.0	1.9835563181227624E-4	0.0	0.0030745122930902814	0.0
10	0.0	1.9835563181227624E-4	0.0	0.004363823899870077	0.0
11	0.0	1.9835563181227624E-4	0.0	0.004958890795306906	0.0
12	0.0	1.9835563181227624E-4	0.0	0.005454779874837596	0.0
13	0.0	1.9835563181227624E-4	0.0	0.005553957690743735	0.0
14	0.0	1.9835563181227624E-4	0.0	0.005553957690743735	0.0
15	0.0	1.9835563181227624E-4	0.0	0.005950668954368287	0.0
16	0.0	1.9835563181227624E-4	0.0	0.007239980561148082	0.0
17	0.0	1.9835563181227624E-4	0.0	0.009620248142895397	0.0
18	0.0	2.9753344771841437E-4	0.0	0.010116137222426087	0.0
19	0.0	2.9753344771841437E-4	0.0	0.010810381933769054	0.0
20	0.0	2.9753344771841437E-4	0.0	0.012793938251891818	0.0
21	0.0	2.9753344771841437E-4	0.0	0.01477749457001458	0.0
22	0.0	2.9753344771841437E-4	0.0	0.01953802973350921	0.0
23	0.0	2.9753344771841437E-4	0.0	0.026579654662845016	0.0
24	0.0	2.9753344771841437E-4	0.0	0.03729085878070793	0.0
25	0.0	2.9753344771841437E-4	0.0	0.040167015441985934	0.0
26	0.0	2.9753344771841437E-4	0.0	0.04443166152594988	0.0
27	0.0	2.9753344771841437E-4	0.0	0.052365886798440925	0.0
28	0.0	2.9753344771841437E-4	0.0	0.07299487250691765	0.0
29	0.0	2.9753344771841437E-4	0.0	0.10711204117862916	0.0
30	0.0	2.9753344771841437E-4	0.0	0.1618581955588174	0.0
31	0.0	2.9753344771841437E-4	0.0	0.380545279631852	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGTT	150	0.0	23.433332	10
TTATACT	80	2.725028E-8	23.125	4
TAGGACC	195	0.0	22.769232	4
GGTATCA	940	0.0	21.648937	1
GCGGTTC	170	0.0	20.676472	11
CGGTTCT	170	0.0	20.676472	12
ATAGGAC	225	0.0	20.555555	3
TCTATAT	55	5.1424914E-4	20.181818	3
GACCGCG	180	0.0	19.527777	7
GGTTCTA	180	0.0	19.527777	13
AGGACCG	210	0.0	19.38095	5
GTTTTCG	190	0.0	18.5	28
ATTATAC	70	1.2190086E-4	18.5	3
TTTCGGA	205	0.0	18.04878	30
TTCGGAA	195	0.0	18.025642	31
TTTTCGG	195	0.0	18.025642	29
TATACTG	105	4.79582E-7	17.619047	5
TCTTATA	790	0.0	17.094936	37
GTATACT	65	0.0015797522	17.076923	4
GCCGTCT	185	1.8189894E-12	17.0	36
>>END_MODULE