FastQCFastQC Report
Fri 10 Feb 2017
ERR1630830.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630830.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033984
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110981.0733241520178263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT107151.0362829598910621No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT90760.8777698687794008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59910.5794093525625155No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT16340.1580295246348106No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15990.15464455929685567No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15720.15203330032186185No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15470.14961546793760833No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT13780.13327092102005447TruSeq Adapter, Index 3 (95% over 21bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11860.11470196830898738No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11500.1112202896756623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT358.868789E-426.4285724
GCCGTCT2150.024.95348736
TCCGCTT603.7258193E-521.58333424
CCCGTGA705.1011048E-621.14285925
CCGTCTT2600.020.63461537
GGTATCA51300.018.0311891
GCCCGTG852.7226875E-517.41176424
GTCATAA650.001579789117.0769231
CCGCTTA650.001579789117.07692325
CGCAAGA650.001579789117.0769232
TGCCCGT904.44526E-516.44444523
CAATACT904.44526E-516.4444454
TGCCGTC3300.016.25757635
GTATATG1606.2937033E-1016.18751
CCAATAC1151.2418222E-616.0869563
TATGCCG3350.016.01492533
CCGCTGA700.00259225715.85714317
GACGGAC700.00259225715.8571437
GCCTAAT700.00259225715.8571431
TCGGTGA2100.015.85714213
Produced by FastQC (version 0.11.5)