##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630830.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1033984
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07220034352562	33.0	31.0	34.0	30.0	34.0
2	32.22062913932904	33.0	31.0	34.0	30.0	34.0
3	32.30801056882892	34.0	31.0	34.0	30.0	34.0
4	35.89052151677395	37.0	35.0	37.0	35.0	37.0
5	35.86654725798465	37.0	35.0	37.0	35.0	37.0
6	35.96021118315177	37.0	35.0	37.0	35.0	37.0
7	35.90787768476108	37.0	35.0	37.0	35.0	37.0
8	35.92940606431047	37.0	35.0	37.0	35.0	37.0
9	37.60617282279029	39.0	37.0	39.0	35.0	39.0
10	37.544848856461996	39.0	37.0	39.0	35.0	39.0
11	37.64822569788314	39.0	37.0	39.0	35.0	39.0
12	37.61426772561278	39.0	37.0	39.0	35.0	39.0
13	37.65602272375588	39.0	37.0	39.0	35.0	39.0
14	38.9113593633944	40.0	38.0	41.0	36.0	41.0
15	38.90611363425353	40.0	38.0	41.0	36.0	41.0
16	38.85315149944293	40.0	38.0	41.0	35.0	41.0
17	38.88943832786581	40.0	38.0	41.0	35.0	41.0
18	38.89983984278287	40.0	38.0	41.0	35.0	41.0
19	38.991508572666504	40.0	38.0	41.0	35.0	41.0
20	38.972684296855654	40.0	39.0	41.0	35.0	41.0
21	38.93590229636049	40.0	38.0	41.0	35.0	41.0
22	38.83730019033177	40.0	38.0	41.0	35.0	41.0
23	38.772813699244864	40.0	38.0	41.0	35.0	41.0
24	38.70618887719733	40.0	38.0	41.0	35.0	41.0
25	38.64654772220847	40.0	38.0	41.0	34.0	41.0
26	38.48270766278782	40.0	38.0	41.0	34.0	41.0
27	38.31476695964348	40.0	38.0	41.0	34.0	41.0
28	38.19499818179005	40.0	37.0	41.0	34.0	41.0
29	38.097087575823224	40.0	37.0	41.0	33.0	41.0
30	37.9591221914459	40.0	37.0	41.0	33.0	41.0
31	37.847608860485266	40.0	37.0	41.0	33.0	41.0
32	37.68376880106462	40.0	36.0	41.0	33.0	41.0
33	37.54285656257737	40.0	36.0	41.0	33.0	41.0
34	37.4319476897128	40.0	36.0	41.0	33.0	41.0
35	37.31173403070067	40.0	36.0	41.0	33.0	41.0
36	37.2115119769745	40.0	35.0	41.0	32.0	41.0
37	37.01425553973756	40.0	35.0	41.0	31.0	41.0
38	36.799488193240904	39.0	35.0	41.0	31.0	41.0
39	36.650375634439214	39.0	35.0	41.0	31.0	41.0
40	36.387725535404805	39.0	35.0	41.0	30.0	41.0
41	36.240027892114384	39.0	35.0	41.0	30.0	41.0
42	35.999089927890566	39.0	35.0	41.0	28.0	41.0
43	35.22327424795741	38.0	35.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	5.0
12	10.0
13	3.0
14	5.0
15	2.0
16	6.0
17	12.0
18	24.0
19	59.0
20	148.0
21	298.0
22	516.0
23	978.0
24	1560.0
25	2537.0
26	3939.0
27	6026.0
28	8669.0
29	12312.0
30	16201.0
31	20836.0
32	26095.0
33	34195.0
34	44441.0
35	58398.0
36	81792.0
37	134092.0
38	218441.0
39	362380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28579165635058	20.719662973508292	13.089854388462491	21.904690981678634
2	18.261984711562267	22.571335726665016	35.16156923124536	24.00511033052736
3	21.412033455063135	25.47844067219609	29.99698254518445	23.112543327556327
4	13.554174919534539	16.368435101510276	34.97887781629116	35.09851216266402
5	12.759481771478088	39.806515381282495	33.50448362837336	13.929519218866057
6	32.024963635800944	35.0477376825947	16.917863332508045	16.00943534909631
7	26.44470320623917	33.0832972270364	21.992119800693242	18.479879766031196
8	28.357788901955928	32.16713217999505	20.701674300569447	18.773404617479574
9	26.728169874969055	13.445565888833869	22.603347827432533	37.22291640876455
10	18.001148953949	28.445798000742755	32.30814016464472	21.24491288066353
11	32.05504147066105	23.5349870017331	21.776836005199307	22.633135522406537
12	20.70854094454073	28.99300182594702	29.135363796731866	21.163093432780393
13	29.097548898242138	21.293076101757862	27.458935534785837	22.150439465214163
14	22.041927147808863	23.377731183461254	28.21552364446645	26.364818024263432
15	25.45977500618965	27.83302256127754	25.9140373545432	20.7931650779896
16	22.772305954444167	27.531373793018073	27.512611413716265	22.183708838821488
17	20.584361073285468	28.32403596187175	28.511176188412975	22.58042677642981
18	20.437743717504333	25.50629410126269	31.179399294379795	22.876562886853183
19	20.841425012379304	27.02836794379797	31.513543729883637	20.616663313939092
20	22.136802890566972	25.633472084674423	31.740336407526616	20.489388617231988
21	23.037493810349098	26.441995233968807	30.106848848724933	20.41366210695717
22	22.744065672196086	26.461918172815054	30.467879580341666	20.32613657464719
23	21.795114818024263	26.49412370017331	30.376485516216885	21.33427596558554
24	21.140365808368408	27.348392238177766	30.452889019559297	21.05835293389453
25	21.124794967813816	27.305644961624164	30.619526027482053	20.95003404307997
26	20.999938103490965	27.753620945778657	30.19418095444417	21.05225999628621
27	21.067830836840802	27.492591761574648	30.64206022530329	20.797517176281257
28	20.356697975984154	27.479922319881155	30.990228088635803	21.173151615498888
29	20.32072063010646	27.784085633820254	31.417410714285715	20.47778302178757
30	19.84682548279277	28.593672629363702	31.303192312453575	20.256309575389945
31	20.711829196583313	28.88458622183709	29.837792461005197	20.5657921205744
32	19.95698192621936	28.218811896509038	30.620106307254268	21.20409987001733
33	19.4551366365437	28.101788809111167	31.102415511265164	21.34065904307997
34	20.541226943550384	27.477020921020056	30.98819710943303	20.993555025996532
35	19.8476959024511	27.726154369893536	31.193326008913097	21.232823718742264
36	19.967426962119337	28.457887162664026	30.069227376825946	21.50545849839069
37	20.39402930799703	27.216765443179003	30.856957167615747	21.53224808120822
38	19.872357792770487	27.012313536766523	31.11237698068829	22.002951689774697
39	19.918780174548157	27.35690300817034	31.42272994553107	21.301586871750434
40	19.173604233721218	26.761052395394902	32.12361119707848	21.9417321738054
41	19.07244212676405	27.057672072295126	31.818093897004207	22.051791903936618
42	19.04661967690022	27.08349452215895	32.017710138648184	21.852175662292648
43	18.44999535776182	26.3192660621441	31.96442111289923	23.26631746719485
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	603.0
1	482.0
2	361.0
3	1040.5
4	1720.0
5	1720.0
6	2549.5
7	3379.0
8	3427.5
9	3476.0
10	5038.5
11	6601.0
12	6601.0
13	11483.5
14	16366.0
15	21620.0
16	26874.0
17	25358.5
18	23843.0
19	23843.0
20	25891.0
21	27939.0
22	23636.5
23	19334.0
24	20378.5
25	21423.0
26	21423.0
27	23387.0
28	25351.0
29	28118.0
30	30885.0
31	34173.5
32	37462.0
33	37462.0
34	41192.5
35	44923.0
36	47419.0
37	49915.0
38	53337.5
39	56760.0
40	56760.0
41	58895.0
42	61030.0
43	62276.0
44	63522.0
45	62737.0
46	61952.0
47	61952.0
48	63282.0
49	64612.0
50	64004.0
51	63396.0
52	62425.0
53	61454.0
54	61454.0
55	59851.0
56	58248.0
57	54667.5
58	51087.0
59	46935.0
60	42783.0
61	42783.0
62	38784.0
63	34785.0
64	30100.0
65	25415.0
66	22104.5
67	18794.0
68	18794.0
69	15467.0
70	12140.0
71	10015.0
72	7890.0
73	5835.0
74	3780.0
75	3780.0
76	2902.0
77	2024.0
78	1745.0
79	1466.0
80	1177.5
81	889.0
82	889.0
83	737.0
84	585.0
85	513.0
86	441.0
87	354.0
88	267.0
89	267.0
90	214.0
91	161.0
92	98.5
93	36.0
94	21.5
95	7.0
96	7.0
97	6.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1033984.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.048732002817395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.084768795546	51.69281210928239
2	8.98468172730649	10.790374903472758
3	2.0062570752693953	3.614195803248245
4	0.7521593874876099	1.8066486993058708
5	0.3978576769586118	1.1945424509475588
6	0.26482693748421826	0.954151307767002
7	0.2054510458278884	0.8635952353432197
8	0.13538771027074992	0.6503888264418679
9	0.12219451113367205	0.6603862906153043
>10	0.8745782660473439	10.515085093706606
>50	0.10327097080830187	4.290471487333296
>100	0.06259662996947607	6.784042792374
>500	0.004194619274134141	1.6352926456731187
>1k	0.0011293205738053455	0.9751691807669203
>5k	3.226630210872416E-4	1.4596536903055024
>10k+	3.226630210872416E-4	2.113189483416335
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11098	1.0733241520178263	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10715	1.0362829598910621	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9076	0.8777698687794008	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5991	0.5794093525625155	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	1634	0.1580295246348106	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1599	0.15464455929685567	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1572	0.15203330032186185	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1547	0.14961546793760833	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	1378	0.13327092102005447	TruSeq Adapter, Index 3 (95% over 21bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1186	0.11470196830898738	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1150	0.1112202896756623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.671329537014113E-5	0.0
2	0.0	0.0	0.0	0.0013539861351819758	0.0
3	0.0	0.0	0.0	0.001547412725922258	0.0
4	0.0	0.0	0.0	0.003191538747214657	0.0
5	0.0	0.0	0.0	0.0032882520425847983	0.0
6	0.0	0.0	0.0	0.0032882520425847983	0.0
7	0.0	0.0	0.0	0.0034816786333250805	0.0
8	0.0	0.0	0.0	0.0035783919286952216	0.0
9	0.0	0.0	0.0	0.00599622431294875	0.0
10	0.0	0.0	0.0	0.008704196583312701	0.0
11	0.0	0.0	0.0	0.010541749195345382	0.0
12	0.0	0.0	0.0	0.012669441693488487	0.0
13	0.0	0.0	0.0	0.013346434761079475	0.0
14	0.0	0.0	0.0	0.015183987373112157	0.0
15	0.0	0.0	0.0	0.01818209952958653	0.0
16	0.0	0.0	0.0	0.026112589749938103	0.0
17	0.0	0.0	0.0	0.0376214718989849	0.0
18	0.0	0.0	0.0	0.04207028348601139	0.0
19	0.0	0.0	0.0	0.047873081208219856	0.0
20	0.0	0.0	0.0	0.052902172567467196	0.0
21	0.0	0.0	0.0	0.06576504085169596	0.0
22	0.0	0.0	0.0	0.08907294503589998	0.0
23	0.0	0.0	0.0	0.12243903193859866	0.0
24	0.0	0.0	0.0	0.17118253280514978	0.0
25	0.0	0.0	0.0	0.18278812824956672	0.0
26	0.0	0.0	0.0	0.2027110670958158	0.0
27	0.0	0.0	0.0	0.23617386729388462	0.0
28	0.0	0.0	0.0	0.293331424857638	0.0
29	0.0	0.0	0.0	0.37099220103986136	0.0
30	0.0	0.0	0.0	0.5097757798960139	0.0
31	0.0	0.0	0.0	0.9317358875959396	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGT	35	8.868789E-4	26.428572	4
GCCGTCT	215	0.0	24.953487	36
TCCGCTT	60	3.7258193E-5	21.583334	24
CCCGTGA	70	5.1011048E-6	21.142859	25
CCGTCTT	260	0.0	20.634615	37
GGTATCA	5130	0.0	18.031189	1
GCCCGTG	85	2.7226875E-5	17.411764	24
GTCATAA	65	0.0015797891	17.076923	1
CCGCTTA	65	0.0015797891	17.076923	25
CGCAAGA	65	0.0015797891	17.076923	2
TGCCCGT	90	4.44526E-5	16.444445	23
CAATACT	90	4.44526E-5	16.444445	4
TGCCGTC	330	0.0	16.257576	35
GTATATG	160	6.2937033E-10	16.1875	1
CCAATAC	115	1.2418222E-6	16.086956	3
TATGCCG	335	0.0	16.014925	33
CCGCTGA	70	0.002592257	15.857143	17
GACGGAC	70	0.002592257	15.857143	7
GCCTAAT	70	0.002592257	15.857143	1
TCGGTGA	210	0.0	15.857142	13
>>END_MODULE