FastQCFastQC Report
Fri 10 Feb 2017
ERR1630829.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630829.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343707
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73590.3139897606654757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69730.29752012516922977No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61540.2625754840515474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40280.17186448647377853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33950.017.764361
GTACTAG1059.35131E-615.8571421
GACGGAC3200.014.4531257
CCTATAC1803.3360266E-914.3888883
TCTAGCG1304.4484204E-614.23076928
GTACCGG1702.4401743E-814.1470591
GCTTAGG7500.014.0599991
CGCGGTT2800.013.87500110
GTCTAGA2009.858923E-1013.8750011
AAGACGG3350.013.8059715
GAGCGAA3000.013.56666616
TAGGACA8350.013.514974
ACGGACC3250.013.0923088
TTAGGAC8100.013.0185183
GTAGAAC6400.013.00781253
TAGGTCA1000.001833467712.9500014
CGAACGA1451.373303E-512.75861916
CGAATTG1451.373303E-512.75861914
CGCTAAG1901.04726496E-712.6578957
AAACGCA2052.068191E-812.63414628
Produced by FastQC (version 0.11.5)