##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630829.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2343707
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.106372938255504	33.0	31.0	34.0	30.0	34.0
2	32.288878259953144	34.0	31.0	34.0	30.0	34.0
3	32.39307601163456	34.0	31.0	34.0	30.0	34.0
4	35.9565291224543	37.0	35.0	37.0	35.0	37.0
5	35.909474605827434	37.0	35.0	37.0	35.0	37.0
6	35.980671218714626	37.0	35.0	37.0	35.0	37.0
7	35.932015819383565	37.0	35.0	37.0	35.0	37.0
8	35.93121495135697	37.0	35.0	37.0	35.0	37.0
9	37.647775511188044	39.0	37.0	39.0	35.0	39.0
10	37.56313694501915	39.0	37.0	39.0	35.0	39.0
11	37.65354116363522	39.0	37.0	39.0	35.0	39.0
12	37.602354304526976	39.0	37.0	39.0	35.0	39.0
13	37.639462612007385	39.0	37.0	39.0	35.0	39.0
14	38.96054967621806	40.0	38.0	41.0	36.0	41.0
15	38.97705045895242	40.0	38.0	41.0	36.0	41.0
16	38.9424403306386	40.0	38.0	41.0	36.0	41.0
17	38.9272929594015	40.0	38.0	41.0	36.0	41.0
18	38.911491069489486	40.0	38.0	41.0	35.0	41.0
19	38.976534609488304	40.0	38.0	41.0	35.0	41.0
20	38.94188736049344	40.0	38.0	41.0	35.0	41.0
21	38.90106570488547	40.0	38.0	41.0	35.0	41.0
22	38.8398656487351	40.0	38.0	41.0	35.0	41.0
23	38.791790526716866	40.0	38.0	41.0	35.0	41.0
24	38.745529197975685	40.0	38.0	41.0	35.0	41.0
25	38.698075740696254	40.0	38.0	41.0	34.0	41.0
26	38.57465758305112	40.0	38.0	41.0	34.0	41.0
27	38.456330505477005	40.0	38.0	41.0	34.0	41.0
28	38.37453231141947	40.0	38.0	41.0	34.0	41.0
29	38.3079902905952	40.0	38.0	41.0	34.0	41.0
30	38.22892708004883	40.0	38.0	41.0	34.0	41.0
31	38.18510206267251	40.0	38.0	41.0	34.0	41.0
32	38.09609605637565	40.0	38.0	41.0	33.0	41.0
33	38.028677646139215	40.0	37.0	41.0	33.0	41.0
34	37.96996211557161	40.0	37.0	41.0	33.0	41.0
35	37.92356553101561	40.0	37.0	41.0	33.0	41.0
36	37.89346236538953	40.0	37.0	41.0	33.0	41.0
37	37.76668286607498	40.0	37.0	41.0	33.0	41.0
38	37.63058564914471	40.0	37.0	41.0	33.0	41.0
39	37.578489973362714	40.0	37.0	41.0	33.0	41.0
40	37.42387337666355	40.0	37.0	41.0	32.0	41.0
41	37.37090771158682	40.0	37.0	41.0	32.0	41.0
42	37.255723518340815	40.0	36.0	41.0	31.0	41.0
43	36.48244511792643	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	8.0
11	7.0
12	3.0
13	2.0
14	1.0
15	4.0
16	6.0
17	27.0
18	65.0
19	104.0
20	253.0
21	475.0
22	911.0
23	1670.0
24	2713.0
25	4595.0
26	6958.0
27	10617.0
28	15418.0
29	21904.0
30	30011.0
31	39716.0
32	51621.0
33	67334.0
34	89732.0
35	121439.0
36	171202.0
37	269402.0
38	511228.0
39	926277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33028829968934	19.219893954321083	12.137396014092205	25.312421731897373
2	18.404988336852686	21.47520999851944	34.877098545167975	25.242703119459897
3	20.369397710549997	23.655089992051053	29.71361181239805	26.2619004850009
4	14.683661396241082	15.551559985953874	34.47013641210271	35.29464220570233
5	14.197337807157634	37.27479586825486	34.40340452112828	14.124461803459221
6	34.62169119262774	34.57531167505154	15.925412178228763	14.877584954091958
7	28.479583838764828	30.825994887586205	21.722297198412598	18.972124075236366
8	26.920387232704428	33.589693592245105	20.49291997677184	18.996999198278626
9	26.67436671904807	14.193711073952503	20.12482789017569	39.00709431682373
10	16.83230881675909	27.236766370540344	33.29123478318749	22.639690029513073
11	34.77866473923575	22.142528908263703	21.886524211430867	21.192282141069683
12	21.35898386615733	25.822169750741025	29.339972957370524	23.478873425731116
13	29.5965323310465	20.28252678342472	25.79528925757358	24.3256516279552
14	22.785698041606736	21.34046619308642	25.58455472463068	30.28928104067616
15	25.308794998692242	27.851092308040208	23.11120801362969	23.728904679637854
16	24.7336377798078	26.85958611720663	24.455872683744172	23.9509034192414
17	23.277440396773148	26.798486329562525	26.04651520006554	23.877558073598788
18	22.92795131814685	25.6111792131013	27.47340004531283	23.987469423439023
19	23.83506129392454	25.845551513051763	27.48453624962506	22.834850943398642
20	24.294803061986844	25.0953724164326	27.435170010585797	23.17465451099476
21	24.15493916261717	25.82477246515883	26.462864172014676	23.557424200209326
22	24.14393096065336	25.520041541028803	26.65256365236781	23.683463845950026
23	23.675613035247153	25.82600982119352	26.8450791843861	23.653297959173226
24	24.11129036180717	25.99591160499158	26.59837599153819	23.294422041663058
25	24.089401960227963	25.483646206629075	26.827713532450943	23.599238300692026
26	23.78266566597275	26.096265446150053	26.62734718972977	23.49372169814742
27	24.014307249157	25.661868143074194	26.417081998731067	23.906742609037735
28	23.360727258142763	25.817134991703316	27.038746737540144	23.783391012613777
29	23.20669776554834	26.28754362213365	27.001754058847798	23.504004553470207
30	23.12716563973227	26.84222046527147	26.843799160901938	23.186814734094323
31	23.640369721983166	26.37019047176119	26.612840256909248	23.3765995493464
32	22.964901329389722	26.253537664904357	26.83296162873601	23.948599376969902
33	23.097511762349132	26.008114495540614	27.03657069761707	23.85780304449319
34	23.559258900536626	25.831897929220677	26.87853046477226	23.730312705470435
35	23.460099747963376	26.032861616234452	27.193245572078762	23.31379306372341
36	23.196372242776082	26.23514799418187	26.86393819705279	23.704541565989263
37	23.794356547128118	25.27444770186717	26.99189787801973	23.939297872984977
38	23.149736720503032	25.552596804976048	27.584378081389865	23.71328839313105
39	23.130323030993207	25.63379296132153	27.49345374656474	23.74243026112052
40	22.779681931231167	25.25806340126987	28.223067132538326	23.73918753496064
41	22.195607215407044	25.594624242706104	28.43896442686735	23.770804115019498
42	22.839459027941633	25.105996611351163	28.132910811803697	23.921633548903507
43	22.197740587880652	25.022240408037355	28.23911009353985	24.540908910542143
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	310.0
1	286.0
2	262.0
3	734.0
4	1206.0
5	1206.0
6	1773.0
7	2340.0
8	2322.5
9	2305.0
10	3404.5
11	4504.0
12	4504.0
13	7685.5
14	10867.0
15	15223.0
16	19579.0
17	19733.0
18	19887.0
19	19887.0
20	23426.0
21	26965.0
22	27721.5
23	28478.0
24	32546.5
25	36615.0
26	36615.0
27	41194.5
28	45774.0
29	55365.0
30	64956.0
31	71600.5
32	78245.0
33	78245.0
34	85264.0
35	92283.0
36	99175.0
37	106067.0
38	117753.5
39	129440.0
40	129440.0
41	136679.5
42	143919.0
43	140637.5
44	137356.0
45	142318.5
46	147281.0
47	147281.0
48	151199.5
49	155118.0
50	159388.5
51	163659.0
52	169961.0
53	176263.0
54	176263.0
55	162794.0
56	149325.0
57	142380.0
58	135435.0
59	123598.5
60	111762.0
61	111762.0
62	104748.5
63	97735.0
64	87487.0
65	77239.0
66	68067.5
67	58896.0
68	58896.0
69	50765.0
70	42634.0
71	36838.5
72	31043.0
73	25104.5
74	19166.0
75	19166.0
76	15222.5
77	11279.0
78	9219.0
79	7159.0
80	5530.0
81	3901.0
82	3901.0
83	2954.0
84	2007.0
85	1619.0
86	1231.0
87	1023.5
88	816.0
89	816.0
90	575.0
91	334.0
92	190.5
93	47.0
94	31.0
95	15.0
96	15.0
97	9.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2343707.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.0823917329923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.4986006492336	46.82801230767003
2	10.124366517859217	11.355973782059435
3	2.7190879591604022	4.574788682462887
4	1.098975649075891	2.4653273142597443
5	0.6056925856673706	1.6984344429583238
6	0.3722092935180037	1.2524632443442196
7	0.2555915734605106	1.0033930722524962
8	0.17903867204821547	0.8032733552930209
9	0.13502889747740657	0.6815469171241774
>10	0.8049624721762901	8.595703796069772
>50	0.09533701083232217	3.788466226406934
>100	0.10103982795420695	11.67039290807824
>500	0.008238183984381281	3.1094176024485156
>1k	0.0016018691080741043	1.296428430105259
>5k	2.2883844401058633E-4	0.8763779184667988
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7359	0.3139897606654757	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6973	0.29752012516922977	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6154	0.2625754840515474	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4028	0.17186448647377853	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2800234841641895E-4	0.0
2	0.0	0.0	0.0	0.0021333724736069823	0.0
3	0.0	0.0	0.0	0.003413395957771172	0.0
4	0.0	0.0	0.0	0.004650751992463222	0.0
5	0.0	0.0	0.0	0.005248096285073177	0.0
6	0.0	0.0	0.0	0.005248096285073177	0.0
7	0.0	0.0	0.0	0.0060587778250438306	0.0
8	0.0	0.0	0.0	0.0066987895671259245	0.0
9	0.0	0.0	0.0	0.010240187873313517	0.0
10	0.0	4.2667449472139646E-5	0.0	0.015146944562609576	0.0
11	4.2667449472139646E-5	4.2667449472139646E-5	0.0	0.016640305294134464	0.0
12	4.2667449472139646E-5	4.2667449472139646E-5	0.0	0.018560340520380747	0.0
13	4.2667449472139646E-5	4.2667449472139646E-5	0.0	0.019712361656128517	0.0
14	4.2667449472139646E-5	4.2667449472139646E-5	0.0	0.021291057286597685	0.0
15	4.2667449472139646E-5	4.2667449472139646E-5	0.0	0.022955087816011133	0.0
16	4.2667449472139646E-5	4.2667449472139646E-5	0.0	0.026752490819031558	0.0
17	4.2667449472139646E-5	4.2667449472139646E-5	0.0	0.029867214630497754	0.0
18	4.2667449472139646E-5	8.533489894427929E-5	0.0	0.0317445824072719	0.0
19	4.2667449472139646E-5	8.533489894427929E-5	0.0	0.034304629375600276	0.0
20	4.2667449472139646E-5	8.533489894427929E-5	0.0	0.03707801359128936	0.0
21	4.2667449472139646E-5	8.533489894427929E-5	0.0	0.04087541659430979	0.0
22	4.2667449472139646E-5	8.533489894427929E-5	0.0	0.046592854823576495	0.0
23	4.2667449472139646E-5	1.2800234841641895E-4	0.0	0.055553019212725825	0.0
24	4.2667449472139646E-5	1.7066979788855858E-4	0.0	0.0663905513786493	0.0
25	4.2667449472139646E-5	1.7066979788855858E-4	0.0	0.07078529867427968	0.0
26	4.2667449472139646E-5	1.7066979788855858E-4	0.0	0.07680140904985137	0.0
27	4.2667449472139646E-5	1.7066979788855858E-4	0.0	0.0900709858356868	0.0
28	4.2667449472139646E-5	1.7066979788855858E-4	0.0	0.11336741324747504	0.0
29	4.2667449472139646E-5	2.9867214630497756E-4	0.0	0.14703203088099323	0.0
30	4.2667449472139646E-5	2.9867214630497756E-4	0.0	0.1963982699202588	0.0
31	4.2667449472139646E-5	2.9867214630497756E-4	0.0	0.3713774802055035	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3395	0.0	17.76436	1
GTACTAG	105	9.35131E-6	15.857142	1
GACGGAC	320	0.0	14.453125	7
CCTATAC	180	3.3360266E-9	14.388888	3
TCTAGCG	130	4.4484204E-6	14.230769	28
GTACCGG	170	2.4401743E-8	14.147059	1
GCTTAGG	750	0.0	14.059999	1
CGCGGTT	280	0.0	13.875001	10
GTCTAGA	200	9.858923E-10	13.875001	1
AAGACGG	335	0.0	13.805971	5
GAGCGAA	300	0.0	13.566666	16
TAGGACA	835	0.0	13.51497	4
ACGGACC	325	0.0	13.092308	8
TTAGGAC	810	0.0	13.018518	3
GTAGAAC	640	0.0	13.0078125	3
TAGGTCA	100	0.0018334677	12.950001	4
CGAACGA	145	1.373303E-5	12.758619	16
CGAATTG	145	1.373303E-5	12.758619	14
CGCTAAG	190	1.04726496E-7	12.657895	7
AAACGCA	205	2.068191E-8	12.634146	28
>>END_MODULE