FastQCFastQC Report
Fri 10 Feb 2017
ERR1630828.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630828.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480345
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG23580.1592871931880744No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG18870.1274702856428738No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA17500.11821568620828252No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT17460.11794547892552074No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC16830.11368971422202256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15980.10794780946333457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15190.10261121562878923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG250.00549684929.5999985
AATACGC451.3234632E-424.6666685
TAATACG753.7422797E-722.24
GACTCGT450.003826319720.5555577
TTAACGG555.144055E-420.18181835
TACGACG1303.274181E-1119.9230775
GGTATCA10600.019.0235841
AGACGTA500.007035816618.49999810
TAACGGC650.001580224617.07692336
AGACGGA2350.016.5319166
ATTATAC1151.2427099E-616.0869563
TCGCCAT4150.016.04819313
CGTATGC1302.590241E-715.65384631
TGCCGTC1302.590241E-715.65384635
TTTCGGA2400.015.41666630
GAGTACG4050.015.0740741
ATGCCGT1353.9740917E-715.07407334
GTATAGA1601.0970325E-815.031251
CGGCCTT4950.014.94949324
AAGACGG2600.014.9423075
Produced by FastQC (version 0.11.5)