##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630828.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1480345
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10871452262817	33.0	31.0	34.0	30.0	34.0
2	32.29877021910433	34.0	31.0	34.0	30.0	34.0
3	32.401490193164435	34.0	31.0	34.0	30.0	34.0
4	35.965140558450905	37.0	35.0	37.0	35.0	37.0
5	35.92317331432876	37.0	35.0	37.0	35.0	37.0
6	35.99066974252624	37.0	35.0	37.0	35.0	37.0
7	35.94771489078559	37.0	35.0	37.0	35.0	37.0
8	35.93948505247088	37.0	35.0	37.0	35.0	37.0
9	37.666605419682575	39.0	37.0	39.0	35.0	39.0
10	37.5847278843783	39.0	37.0	39.0	35.0	39.0
11	37.66867926057777	39.0	37.0	39.0	35.0	39.0
12	37.61963326116547	39.0	37.0	39.0	35.0	39.0
13	37.64315345409347	39.0	37.0	39.0	35.0	39.0
14	38.9798060587228	40.0	38.0	41.0	36.0	41.0
15	39.00251292772968	40.0	38.0	41.0	36.0	41.0
16	38.9707851885878	40.0	38.0	41.0	36.0	41.0
17	38.945311397005426	40.0	38.0	41.0	36.0	41.0
18	38.91522584262452	40.0	38.0	41.0	35.0	41.0
19	38.98920927216291	40.0	38.0	41.0	35.0	41.0
20	38.95665537425398	40.0	38.0	41.0	35.0	41.0
21	38.91860681125008	40.0	38.0	41.0	35.0	41.0
22	38.86096686920954	40.0	38.0	41.0	35.0	41.0
23	38.81761886587248	40.0	38.0	41.0	35.0	41.0
24	38.77385271676535	40.0	38.0	41.0	35.0	41.0
25	38.729752861664004	40.0	38.0	41.0	35.0	41.0
26	38.62222792659819	40.0	38.0	41.0	34.0	41.0
27	38.50430946840095	40.0	38.0	41.0	34.0	41.0
28	38.42812114743523	40.0	38.0	41.0	34.0	41.0
29	38.37711479418649	40.0	38.0	41.0	34.0	41.0
30	38.3199004286163	40.0	38.0	41.0	34.0	41.0
31	38.30043334492973	40.0	38.0	41.0	34.0	41.0
32	38.22423016256346	40.0	38.0	41.0	34.0	41.0
33	38.17250640897899	40.0	38.0	41.0	33.0	41.0
34	38.13864943644894	40.0	38.0	41.0	33.0	41.0
35	38.106834555458356	40.0	38.0	41.0	33.0	41.0
36	38.09746038930114	40.0	38.0	41.0	33.0	41.0
37	38.00375520571218	40.0	37.0	41.0	33.0	41.0
38	37.87831822987209	40.0	37.0	41.0	33.0	41.0
39	37.85444271436726	40.0	37.0	41.0	33.0	41.0
40	37.72918407533379	40.0	37.0	41.0	33.0	41.0
41	37.70916982189962	40.0	37.0	41.0	33.0	41.0
42	37.62172399001584	40.0	37.0	41.0	33.0	41.0
43	36.841227551685584	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	2.0
13	1.0
14	2.0
15	3.0
16	4.0
17	17.0
18	31.0
19	71.0
20	123.0
21	277.0
22	504.0
23	866.0
24	1632.0
25	2503.0
26	3945.0
27	5884.0
28	8736.0
29	12402.0
30	17543.0
31	23626.0
32	30862.0
33	40423.0
34	54718.0
35	75137.0
36	106645.0
37	164875.0
38	325851.0
39	603656.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13460713549882	18.82459831998622	12.084818066058926	25.955976478456034
2	18.44860488602319	21.052052055433023	35.13728218759816	25.362060870945623
3	20.117405064359996	23.11217993102959	29.73367694692791	27.036738057682502
4	14.807696854449471	15.34547689896612	34.26660677071899	35.58021947586542
5	14.267417392567271	37.091286152890035	34.587140159895156	14.054156294647532
6	35.166261918674365	34.53411198065316	15.727077134046455	14.572548966626023
7	29.143476689555474	30.387038156645918	21.254504862042296	19.214980291756316
8	26.60636540806366	34.14264917975201	20.212382924250765	19.038602487933556
9	26.50186274145554	14.349695510168237	19.280167798722594	39.86827394965363
10	16.608966153160242	27.06835230976563	33.224417281106774	23.09826425596736
11	35.73315679790859	21.722706531247784	21.636915718970915	20.907220951872706
12	21.698590531261296	25.30653327433808	28.701079815853735	24.29379637854689
13	29.777585630376702	19.93488004485441	25.33125724071078	24.956277084058108
14	23.04591159493226	20.843384481320232	24.60318371730914	31.507520206438365
15	25.572484792396367	27.82966132894697	22.255757948316102	24.342095930340562
16	25.658748467418068	26.534895581773167	23.259240244672693	24.54711570613607
17	24.199291381400958	26.50159253417278	24.7449074371177	24.554208647308567
18	23.46459777957165	25.385028489980378	26.466330483772367	24.684043246675603
19	24.715927706041498	25.615380198534798	26.069260881753912	23.599431213669785
20	25.014506753493272	24.81759319618062	26.164171189823993	24.003728860502115
21	24.85346321296725	25.709817643860045	24.8856178796159	24.551101263556806
22	24.97816387396181	25.240670249164893	24.989309924375736	24.79185595249756
23	24.23164870351168	25.73785164944658	25.325245128669334	24.705254518372406
24	25.159608064336354	25.768655279681425	25.099216736639097	23.972519919343128
25	25.008359537810442	25.139680277232674	25.348145195883394	24.503814989073494
26	24.860556154139747	25.73433895477068	25.07422256298363	24.33088232810595
27	25.08165326325958	24.997956557424114	24.943374686306232	24.977015493010075
28	24.1318071125312	25.313761319151958	25.831816232026995	24.722615336289852
29	24.122282305813847	25.901056848234706	25.516551884864676	24.460108961086775
30	23.706500849464145	26.663784455650543	25.505540938092135	24.12417375679318
31	24.74180005336594	25.78250340292297	25.20405716235067	24.271639381360426
32	23.794453320003107	25.782435851102274	25.329838652476283	25.093272176418335
33	24.052095964116475	25.284849139896444	25.77926091552983	24.88379398045726
34	24.570285980632896	25.09550138650112	25.67725766628725	24.656954966578738
35	24.503409678149353	25.20480023237826	25.92740205830398	24.36438803116841
36	23.944688569218663	25.776356187240136	25.721909419763637	24.557045823777564
37	25.00761646778285	24.5850798293641	25.57775383441022	24.82954986844283
38	23.999743303081374	24.99437631092752	26.54712246131814	24.458757924672963
39	24.203209387001003	24.784425252221613	26.34466965470887	24.667695706068518
40	23.681371572167297	24.327639840712806	27.271413082761114	24.719575504358783
41	22.983088401690146	24.935065812361305	27.548172892129873	24.533672893818668
42	23.930097375949526	24.15146469235212	27.25466023122988	24.663777700468472
43	23.0244301159527	24.141399471069246	27.454343413190845	25.379826999787213
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	39.0
2	41.0
3	150.5
4	260.0
5	260.0
6	357.0
7	454.0
8	477.5
9	501.0
10	763.0
11	1025.0
12	1025.0
13	1690.5
14	2356.0
15	3719.5
16	5083.0
17	5522.0
18	5961.0
19	5961.0
20	7090.0
21	8219.0
22	9932.5
23	11646.0
24	13941.5
25	16237.0
26	16237.0
27	19145.5
28	22054.0
29	29439.5
30	36825.0
31	40221.5
32	43618.0
33	43618.0
34	48525.0
35	53432.0
36	58177.0
37	62922.0
38	72855.5
39	82789.0
40	82789.0
41	89480.5
42	96172.0
43	92537.5
44	88903.0
45	93672.5
46	98442.0
47	98442.0
48	102144.0
49	105846.0
50	110549.5
51	115253.0
52	121924.5
53	128596.0
54	128596.0
55	115730.0
56	102864.0
57	96447.5
58	90031.0
59	81793.0
60	73555.0
61	73555.0
62	69185.0
63	64815.0
64	57427.5
65	50040.0
66	44055.5
67	38071.0
68	38071.0
69	32450.0
70	26829.0
71	23196.5
72	19564.0
73	15535.0
74	11506.0
75	11506.0
76	9089.5
77	6673.0
78	5611.0
79	4549.0
80	3530.5
81	2512.0
82	2512.0
83	1852.0
84	1192.0
85	976.5
86	761.0
87	614.5
88	468.0
89	468.0
90	339.5
91	211.0
92	116.5
93	22.0
94	15.0
95	8.0
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1480345.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.126971861288744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.21337896427985	47.52682516230479
2	8.153721708945726	8.989799744276604
3	2.180306478405333	3.60581081652109
4	0.9360309007222931	2.0640219650165843
5	0.5412727074405387	1.4919362656179074
6	0.33884793840979055	1.1207796459583337
7	0.23447609140979114	0.9048169825304767
8	0.16736801761584008	0.7381193598070476
9	0.137935304788918	0.6843560099199273
>10	0.8487898632084829	9.017491198248306
>50	0.1116916585099029	4.307071323539271
>100	0.12402363702028753	13.957708018479718
>500	0.009209643366133797	3.379249540641644
>1k	0.002947085877162815	2.212013967138361
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2358	0.1592871931880744	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1887	0.1274702856428738	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1750	0.11821568620828252	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1746	0.11794547892552074	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1683	0.11368971422202256	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1598	0.10794780946333457	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1519	0.10261121562878923	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.0265546207134147E-4	0.0
2	0.0	0.0	0.0	0.001688795517261179	0.0
3	0.0	0.0	0.0	0.003107383751760569	0.0
4	0.0	0.0	0.0	0.00594456022075935	0.0
5	0.0	0.0	0.0	0.006484974786282927	0.0
6	0.0	0.0	0.0	0.006620078427663822	0.0
7	0.0	0.0	0.0	0.007430700275949187	0.0
8	0.0	0.0	0.0	0.008038666662163211	6.755182069044716E-5
9	0.0	0.0	0.0	0.013172605034637196	6.755182069044716E-5
10	0.0	0.0	0.0	0.02121127169680041	1.3510364138089433E-4
11	0.0	0.0	0.0	0.0237782408830374	1.3510364138089433E-4
12	0.0	0.0	0.0	0.025399484579608132	1.3510364138089433E-4
13	0.0	0.0	0.0	0.026547865531345735	1.3510364138089433E-4
14	0.0	0.0	0.0	0.027898901945154677	1.3510364138089433E-4
15	0.0	0.0	0.0	0.029857904745177643	1.3510364138089433E-4
16	0.0	0.0	0.0	0.032154666648652845	1.3510364138089433E-4
17	0.0	0.0	0.0	0.034383876731437606	1.3510364138089433E-4
18	0.0	0.0	0.0	0.03647798317284147	1.3510364138089433E-4
19	0.0	0.0	0.0	0.03816677869010265	1.3510364138089433E-4
20	0.0	0.0	0.0	0.04181457700738679	2.0265546207134147E-4
21	0.0	0.0	0.0	0.04492196075914736	2.0265546207134147E-4
22	0.0	0.0	0.0	0.04992079549024045	2.0265546207134147E-4
23	0.0	0.0	0.0	0.054109008373048176	2.0265546207134147E-4
24	0.0	0.0	0.0	0.061134397724854676	2.0265546207134147E-4
25	0.0	0.0	0.0	0.06498485150421017	2.0265546207134147E-4
26	0.0	0.0	0.0	0.06930816802839879	2.0265546207134147E-4
27	0.0	0.0	0.0	0.077346834690562	2.0265546207134147E-4
28	0.0	0.0	0.0	0.09930117641495732	2.0265546207134147E-4
29	0.0	0.0	0.0	0.13030746211187258	2.0265546207134147E-4
30	0.0	0.0	0.0	0.17678311474690023	2.0265546207134147E-4
31	0.0	0.0	0.0	0.34201486815573395	2.0265546207134147E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	25	0.005496849	29.599998	5
AATACGC	45	1.3234632E-4	24.666668	5
TAATACG	75	3.7422797E-7	22.2	4
GACTCGT	45	0.0038263197	20.555557	7
TTAACGG	55	5.144055E-4	20.181818	35
TACGACG	130	3.274181E-11	19.923077	5
GGTATCA	1060	0.0	19.023584	1
AGACGTA	50	0.0070358166	18.499998	10
TAACGGC	65	0.0015802246	17.076923	36
AGACGGA	235	0.0	16.531916	6
ATTATAC	115	1.2427099E-6	16.086956	3
TCGCCAT	415	0.0	16.048193	13
CGTATGC	130	2.590241E-7	15.653846	31
TGCCGTC	130	2.590241E-7	15.653846	35
TTTCGGA	240	0.0	15.416666	30
GAGTACG	405	0.0	15.074074	1
ATGCCGT	135	3.9740917E-7	15.074073	34
GTATAGA	160	1.0970325E-8	15.03125	1
CGGCCTT	495	0.0	14.949493	24
AAGACGG	260	0.0	14.942307	5
>>END_MODULE