FastQCFastQC Report
Fri 10 Feb 2017
ERR1630827.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630827.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271068
Sequences flagged as poor quality0
Sequence length43
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT131914.8663066094116605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT119454.40664335148376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91603.379225876901737No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57712.1289860846724804No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT33671.242123747546741No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT25340.9348207829769651TruSeq Adapter, Index 5 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15880.5858308616288164No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.5363967712898609No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14160.5223781486564257No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG13320.49138961441409534No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC13320.49138961441409534No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC12910.4762642584148627No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10910.4024820340283619No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10010.3692800330544365No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9370.3456697212507563No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7610.2807413637906356No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA7510.2770522525713105No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA7470.2755766080835805No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7120.2626647188159429No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6810.25122847403603527No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6520.24053005149999263No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5890.21728865081824486No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA5830.21507518408664986No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC5710.2106482506234598Illumina Paired End PCR Primer 2 (95% over 23bp)
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5710.2106482506234598No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5470.2017943836970797No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.19515398350229463No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA4710.1737571384302094No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4660.1719125828205469No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4490.16564109374769428No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4430.16342762701609928No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4410.16268980477223427No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA4290.1582628713090442No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA4150.15309811560198916No Hit
GAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA3910.14424424867560906No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG3670.135390381749229No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA3550.13096344828603892No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC3500.1291188926763764No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3370.12432304809125386No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA2980.10993551433588619No Hit
GTACATGGGAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAA2910.10735313648235867No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA2780.10255729189723611No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG2770.10218838077530362No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA2730.1007127362875736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCC200.001839456137.08
CCGTCTT2850.027.91228337
TTTGAGC405.9236518E-527.753
GCCGTCT2900.027.43103436
CGTCTTC601.3311164E-624.66666637
GAAGAGA400.001927402923.1251
GGGAAAT1155.456968E-1222.521747
TTGAGCT502.6947266E-422.24
TATACAC7250.021.9448263
GAAATGG705.0782255E-621.1428579
GGAAATG1151.3278623E-1020.9130448
TTATACA7900.020.139242
TATGCCG4050.019.64197533
TGCCGTC4100.019.40244135
GGGCAGT2100.019.380957
TACACAT8250.019.284855
ATACACA8350.019.0538924
CAGTCGG1950.018.9743610
AGTCGGT1950.018.9743611
TGGGCAG2250.018.9111126
Produced by FastQC (version 0.11.5)