##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630827.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	271068
Sequences flagged as poor quality	0
Sequence length	43
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.887655496037894	33.0	31.0	34.0	30.0	34.0
2	31.898044033231514	33.0	31.0	34.0	30.0	34.0
3	31.8704753050895	33.0	31.0	34.0	30.0	34.0
4	35.59184411291631	37.0	35.0	37.0	33.0	37.0
5	35.63385571148199	37.0	35.0	37.0	33.0	37.0
6	35.80163648973689	37.0	35.0	37.0	35.0	37.0
7	35.67608865672082	37.0	35.0	37.0	33.0	37.0
8	35.80854250593947	37.0	35.0	37.0	35.0	37.0
9	37.32215901545	39.0	37.0	39.0	34.0	39.0
10	37.27327460268272	39.0	37.0	39.0	34.0	39.0
11	37.441863296294656	39.0	37.0	39.0	35.0	39.0
12	37.44917880384258	39.0	37.0	39.0	35.0	39.0
13	37.51844924520785	39.0	37.0	39.0	35.0	39.0
14	38.48453524576859	40.0	38.0	41.0	34.0	41.0
15	38.41075671049331	40.0	38.0	41.0	34.0	41.0
16	38.26454247642658	40.0	37.0	41.0	34.0	41.0
17	38.526554222556705	40.0	38.0	41.0	34.0	41.0
18	38.67797748166512	40.0	38.0	41.0	35.0	41.0
19	38.84745525108091	40.0	38.0	41.0	35.0	41.0
20	38.88433898505172	40.0	38.0	41.0	35.0	41.0
21	38.85219206988652	40.0	38.0	41.0	35.0	41.0
22	38.688163117741674	40.0	38.0	41.0	35.0	41.0
23	38.542716218808565	40.0	38.0	41.0	35.0	41.0
24	38.40133472043915	40.0	38.0	41.0	34.0	41.0
25	38.27591969542698	40.0	37.0	41.0	34.0	41.0
26	37.877266958844274	40.0	36.0	41.0	33.0	41.0
27	37.493994126934936	39.0	35.0	41.0	33.0	41.0
28	37.26398910974368	39.0	35.0	41.0	33.0	41.0
29	36.923513657089735	39.0	35.0	41.0	33.0	41.0
30	36.50976876650877	38.0	35.0	40.0	32.0	41.0
31	36.01839390853956	37.0	35.0	40.0	31.0	41.0
32	35.514892941992414	36.0	35.0	40.0	30.0	41.0
33	35.040299850959904	35.0	35.0	40.0	30.0	41.0
34	34.6688690660646	35.0	35.0	40.0	27.0	41.0
35	34.19982439830596	35.0	34.0	40.0	24.0	41.0
36	33.76010816474095	35.0	33.0	40.0	21.0	41.0
37	33.13019980226364	35.0	33.0	40.0	18.0	41.0
38	32.50377396077737	35.0	33.0	39.0	13.0	41.0
39	31.90636297903109	35.0	33.0	39.0	10.0	41.0
40	31.09609396904098	35.0	31.0	39.0	8.0	41.0
41	30.417341774019803	35.0	28.0	39.0	7.0	41.0
42	29.61122301415143	35.0	23.0	38.0	7.0	41.0
43	28.650220608850915	35.0	20.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	7.0
12	5.0
13	2.0
14	7.0
15	1.0
16	5.0
17	9.0
18	24.0
19	60.0
20	93.0
21	225.0
22	413.0
23	704.0
24	1088.0
25	1719.0
26	2707.0
27	3987.0
28	5360.0
29	7189.0
30	8685.0
31	10032.0
32	11793.0
33	14345.0
34	17525.0
35	20862.0
36	28309.0
37	53169.0
38	45662.0
39	37074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.31122817890714	19.038396269570732	15.47951067628787	13.170864875234258
2	15.288414715126834	28.534906370357255	33.41264922454882	22.764029689967096
3	24.177328198090517	32.475983885962194	31.791284843655465	11.555403072291824
4	12.140127200554844	18.40718933994422	27.1632948190122	42.28938864048873
5	9.064146265881625	47.939631384006965	28.081514601502207	14.914707748609205
6	27.711865657325834	29.606224268449242	20.194932636829137	22.486977437395783
7	20.647586583440315	39.417046645122255	21.635530567975565	18.29983620346186
8	36.84352265852111	27.464326294509128	20.31778004043266	15.374371006537105
9	28.985346850236844	9.905263623887734	24.111662018386532	36.9977275074889
10	20.608850915637404	35.33283161420751	23.62580607080142	20.43251139935367
11	24.090265173314446	27.61078400991633	19.149807428394354	29.14914338837487
12	18.531512388035473	38.364543214248826	27.742854191568167	15.361090206147535
13	30.8417076158012	20.50518689037437	32.34170023757876	16.311405256245664
14	23.14695943453303	25.527911815485417	34.97129871471365	16.3538300352679
15	26.438753375536766	25.61755721811501	32.443150796110196	15.50053861023802
16	17.72064574202783	25.78024702288725	36.2112089955288	20.287898239556128
17	13.777354759691296	34.54557528000354	34.59316481473284	17.083905145572327
18	13.181194386648368	24.166629775554473	40.98565673557926	21.666519102217894
19	13.619829710626115	30.314902533681586	44.12066344976168	11.944604305930614
20	15.033128218749539	24.704871102453996	45.63799489427007	14.624005784526393
21	19.25420927590125	25.34788318798235	42.618457361252524	12.779450174863872
22	17.312998952292414	28.55040063747842	40.88641964377942	13.250180766449745
23	14.7398438768132	29.33691914943852	41.512461817698885	14.410775156049404
24	14.25546357371582	31.331621585727564	41.91383711836145	12.49907772219517
25	11.842047014033378	31.43048976640548	41.71610075700562	15.011362462555521
26	15.634084436377588	30.37466613543465	41.84706420529166	12.1441852228961
27	11.718092877064059	33.759425679165375	41.362683902194284	13.159797541576285
28	12.032036241828619	32.062065607153926	43.946906311331475	11.958991839685982
29	11.95419599510086	34.05971933241843	42.70072454144348	11.285360131037232
30	10.300736346599377	34.007333953104016	43.38247229477474	12.309457405521862
31	11.185385216993522	34.28364838343146	42.301931618634434	12.229034780940577
32	11.07913881387696	35.508802219369315	42.1255921023507	11.286466864403028
33	9.694615373264273	36.40783862351882	42.79110776631694	11.106438236899967
34	11.702967521064824	36.663125119896115	39.58490120560155	12.049006153437514
35	9.700886862337125	35.66263815721516	41.2959109891245	13.34056399132321
36	11.73912081101421	36.030811456903805	38.05502678294745	14.175040949134535
37	10.053934806026533	37.06154913158322	39.04149512299497	13.84302093939528
38	10.608777133413017	35.15538536455797	39.378310977319344	14.857526524709666
39	11.458010536101643	35.59254504404799	38.69914560184161	14.250298818008766
40	10.353859548157658	35.27159236796671	40.80120117461301	13.573346909262622
41	10.617631000339397	36.62844747443446	37.172960290406834	15.580961234819307
42	11.04224770168371	35.50990895273511	38.59880177667596	14.84904156890522
43	9.997491404370859	36.14701846031254	37.61233343662845	16.243156698688153
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21.0
1	76.5
2	132.0
3	623.0
4	1114.0
5	1114.0
6	1915.0
7	2716.0
8	3062.5
9	3409.0
10	4849.5
11	6290.0
12	6290.0
13	10931.5
14	15573.0
15	23018.5
16	30464.0
17	27055.0
18	23646.0
19	23646.0
20	24287.0
21	24928.0
22	17946.0
23	10964.0
24	9572.5
25	8181.0
26	8181.0
27	8552.5
28	8924.0
29	8835.5
30	8747.0
31	8605.5
32	8464.0
33	8464.0
34	7624.5
35	6785.0
36	6262.0
37	5739.0
38	6305.0
39	6871.0
40	6871.0
41	7668.0
42	8465.0
43	8960.5
44	9456.0
45	11141.5
46	12827.0
47	12827.0
48	13340.0
49	13853.0
50	11631.0
51	9409.0
52	9890.5
53	10372.0
54	10372.0
55	7377.5
56	4383.0
57	6223.0
58	8063.0
59	6962.0
60	5861.0
61	5861.0
62	4531.0
63	3201.0
64	3127.0
65	3053.0
66	2859.0
67	2665.0
68	2665.0
69	2393.0
70	2121.0
71	1864.5
72	1608.0
73	1411.5
74	1215.0
75	1215.0
76	1001.5
77	788.0
78	596.5
79	405.0
80	314.0
81	223.0
82	223.0
83	162.0
84	101.0
85	61.0
86	21.0
87	13.5
88	6.0
89	6.0
90	4.0
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	271068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.98006035057694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.40399999601378	29.733447722803774
2	9.828999999512703	7.26954026335601
3	3.254999999838625	3.6111028930548086
4	1.6310000048550422	2.41257914445323
5	0.9689999999736828	1.791683923936792
6	0.6649999999671413	1.475504407915113
7	0.4699999999766991	1.2166439854736648
8	0.3519999999825487	1.0413584994206189
9	0.2829999999859696	0.9418821370824985
>10	1.8069999999104136	12.957813146199745
>50	0.17999999999107605	4.608455120660423
>100	0.12799999999365408	8.86449026619732
>500	0.012999999999355492	3.1917490087000564
>1k	0.009999999999504225	6.066948700814869
>5k	0.001999999999900845	5.521492810670901
>10k+	0.001999999999900845	9.295307969260183
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13191	4.8663066094116605	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11945	4.40664335148376	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9160	3.379225876901737	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5771	2.1289860846724804	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	3367	1.242123747546741	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	2534	0.9348207829769651	TruSeq Adapter, Index 5 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1588	0.5858308616288164	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1454	0.5363967712898609	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1416	0.5223781486564257	No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	1332	0.49138961441409534	No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	1332	0.49138961441409534	No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	1291	0.4762642584148627	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1091	0.4024820340283619	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1001	0.3692800330544365	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	937	0.3456697212507563	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	761	0.2807413637906356	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	751	0.2770522525713105	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	747	0.2755766080835805	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	712	0.2626647188159429	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	681	0.25122847403603527	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	652	0.24053005149999263	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	589	0.21728865081824486	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	583	0.21507518408664986	No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	571	0.2106482506234598	Illumina Paired End PCR Primer 2 (95% over 23bp)
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	571	0.2106482506234598	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	547	0.2017943836970797	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	529	0.19515398350229463	No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA	471	0.1737571384302094	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	466	0.1719125828205469	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	449	0.16564109374769428	No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	443	0.16342762701609928	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	441	0.16268980477223427	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	429	0.1582628713090442	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	415	0.15309811560198916	No Hit
GAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA	391	0.14424424867560906	No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG	367	0.135390381749229	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	355	0.13096344828603892	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	350	0.1291188926763764	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	337	0.12432304809125386	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	298	0.10993551433588619	No Hit
GTACATGGGAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAA	291	0.10735313648235867	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	278	0.10255729189723611	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	277	0.10218838077530362	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	273	0.1007127362875736	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.004426933463190048	0.0
3	0.0	0.0	0.0	0.009591689170245105	0.0
4	0.0	0.0	0.0	0.014387533755367657	0.0
5	0.0	0.0	0.0	0.01660100048696268	0.0
6	0.0	0.0	0.0	0.01733882273082769	0.0
7	0.0	0.0	0.0	0.018445556096625203	0.0
8	0.0	0.0	0.0	0.01918337834049021	0.0
9	0.0	0.0	0.0	0.02840615638880281	0.0
10	0.0	0.0	0.0	0.042424779022237964	0.0
11	0.0	0.0	0.0	0.048696268095090534	0.0
12	0.0	0.0	0.0	0.05238537931441557	0.0
13	0.0	0.0	0.0	0.05607449053374061	0.0
14	0.0	0.0	0.0	0.0645594463381882	0.0
15	0.0	0.0	0.0	0.07452004663036581	0.0
16	0.0	0.0	0.0	0.09997491404370859	0.0
17	0.0	0.0	0.0	0.13133235940797144	0.0
18	0.0	0.0	0.0	0.14387533755367657	0.0
19	0.0	0.0	0.0	0.16969911608895186	0.0
20	0.0	0.0	0.0	0.18962031667330706	0.0
21	0.0	0.0	0.0	0.23093836232974752	0.0
22	0.0	0.0	0.0	0.3139433647645609	0.0
23	0.0	0.0	0.0	0.44785810202605986	0.0
24	0.0	0.0	0.0	0.6186639514808092	0.0
25	0.0	0.0	0.0	0.6540794191863296	0.0
26	0.0	0.0	0.0	0.7289683769386279	0.0
27	0.0	0.0	0.0	0.7784024672775834	0.0
28	0.0	0.0	0.0	0.8510779582982868	0.0
29	0.0	0.0	0.0	0.9558487169271179	0.0
30	0.0	0.0	0.0	1.194534212817448	0.0
31	0.0	0.0	0.0	1.8047132084938096	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGTCC	20	0.0018394561	37.0	8
CCGTCTT	285	0.0	27.912283	37
TTTGAGC	40	5.9236518E-5	27.75	3
GCCGTCT	290	0.0	27.431034	36
CGTCTTC	60	1.3311164E-6	24.666666	37
GAAGAGA	40	0.0019274029	23.125	1
GGGAAAT	115	5.456968E-12	22.52174	7
TTGAGCT	50	2.6947266E-4	22.2	4
TATACAC	725	0.0	21.944826	3
GAAATGG	70	5.0782255E-6	21.142857	9
GGAAATG	115	1.3278623E-10	20.913044	8
TTATACA	790	0.0	20.13924	2
TATGCCG	405	0.0	19.641975	33
TGCCGTC	410	0.0	19.402441	35
GGGCAGT	210	0.0	19.38095	7
TACACAT	825	0.0	19.28485	5
ATACACA	835	0.0	19.053892	4
CAGTCGG	195	0.0	18.97436	10
AGTCGGT	195	0.0	18.97436	11
TGGGCAG	225	0.0	18.911112	6
>>END_MODULE