FastQCFastQC Report
Fri 10 Feb 2017
ERR1630826.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630826.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185558
Sequences flagged as poor quality0
Sequence length43
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75734.081203720669548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73023.9351577404369524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52752.842776921501633No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31591.7024326625637267No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT14870.8013666885825456No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT13590.7323855613878141TruSeq Adapter, Index 10 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8990.4844846355317475No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.4516108171030082No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7920.4268207245174016No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC7240.39017450069520043No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC7020.378318369458606No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG6210.3346662499056898No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5700.307181582039039No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5390.29047521529656495No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.25867922698024337No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA4800.25867922698024337No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.24143394518156047No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4060.21879951282078916No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3830.20640446652798586No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.20370989124694167No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3660.1972429105724356No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.18484786427963223No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3340.17999762877375267No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA3290.17730305349270847No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC3280.17676413843649963RNA PCR Primer, Index 10 (95% over 22bp)
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3040.16383017708748748No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA2870.15466862113193716No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2480.1336509339397924No Hit
GAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA2290.12341154787182443No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.12179480270319792No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2230.12017805753457139No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2220.11963914247836256No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA2180.11748348225352719No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2130.11478890697248299No Hit
GTACATGGGAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAA2050.11047758652281228No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1980.1067051811293504No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1900.10239386067967966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCAAG250.00548461929.619
ATAAGCC250.00548461929.63
TAAGCCT250.00548461929.64
TCAACTG250.00548461929.632
GTTATAC250.00548461929.63
CGTCTTC453.984489E-628.77777737
GCCGTCT1800.026.72222136
CCGTCTT1850.026.00000237
TTTAACC400.001925122823.12500237
GGGGAGC502.6902868E-422.220
AGCTGTG502.6902868E-422.211
TGCCGTC2250.021.37777735
TATACAC3100.020.8870963
ATGCCGT2400.020.812534
GCTGTGG555.1207875E-420.18181812
TATGCCG2600.019.92307733
ATACACA3450.018.7681164
TACACAT3550.018.7605635
TGTGGTA609.1970817E-418.514
TGTTCCC1306.8394E-1018.55
Produced by FastQC (version 0.11.5)