##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630826.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	185558
Sequences flagged as poor quality	0
Sequence length	43
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.929914096940042	33.0	31.0	34.0	30.0	34.0
2	31.968759094191572	33.0	31.0	34.0	30.0	34.0
3	31.94240614794296	33.0	31.0	34.0	30.0	34.0
4	35.64429989545048	37.0	35.0	37.0	33.0	37.0
5	35.67659707476908	37.0	35.0	37.0	35.0	37.0
6	35.831820778408904	37.0	35.0	37.0	35.0	37.0
7	35.72801496028196	37.0	35.0	37.0	35.0	37.0
8	35.832101014238134	37.0	35.0	37.0	35.0	37.0
9	37.373231011327995	39.0	37.0	39.0	34.0	39.0
10	37.33626143847207	39.0	37.0	39.0	34.0	39.0
11	37.485222949158754	39.0	37.0	39.0	35.0	39.0
12	37.46651720755774	39.0	37.0	39.0	35.0	39.0
13	37.53149419588485	39.0	37.0	39.0	35.0	39.0
14	38.554365750870346	40.0	38.0	41.0	34.0	41.0
15	38.503912523308074	40.0	38.0	41.0	34.0	41.0
16	38.34743853673784	40.0	38.0	41.0	34.0	41.0
17	38.580346845730176	40.0	38.0	41.0	34.0	41.0
18	38.70788648293256	40.0	38.0	41.0	35.0	41.0
19	38.858340788324945	40.0	38.0	41.0	35.0	41.0
20	38.89886720055185	40.0	38.0	41.0	35.0	41.0
21	38.83093695771672	40.0	38.0	41.0	35.0	41.0
22	38.699145280720856	40.0	38.0	41.0	35.0	41.0
23	38.575006197523145	40.0	38.0	41.0	35.0	41.0
24	38.4520958406536	40.0	38.0	41.0	34.0	41.0
25	38.34519126095345	40.0	37.0	41.0	34.0	41.0
26	37.997860507226854	40.0	37.0	41.0	34.0	41.0
27	37.6887334418349	40.0	35.0	41.0	33.0	41.0
28	37.47568415266386	39.0	35.0	41.0	33.0	41.0
29	37.22035158818267	39.0	35.0	41.0	33.0	41.0
30	36.854967180073075	38.0	35.0	41.0	32.0	41.0
31	36.43495295271559	38.0	35.0	40.0	32.0	41.0
32	36.01829077700773	37.0	35.0	40.0	31.0	41.0
33	35.61745653650072	37.0	35.0	40.0	30.0	41.0
34	35.304028928960214	36.0	35.0	40.0	30.0	41.0
35	34.9607238707035	36.0	35.0	40.0	27.0	41.0
36	34.59847594822104	35.0	35.0	40.0	24.0	41.0
37	34.0629021653607	35.0	34.0	40.0	21.0	41.0
38	33.531596589745526	35.0	33.0	40.0	18.0	41.0
39	33.05604716584572	35.0	33.0	40.0	15.0	41.0
40	32.38081893531942	35.0	33.0	40.0	10.0	41.0
41	31.795007490919282	35.0	32.0	40.0	9.0	41.0
42	31.12647258539109	35.0	30.0	39.0	7.0	41.0
43	30.194553724441953	35.0	26.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	2.0
12	5.0
13	2.0
14	5.0
15	2.0
16	4.0
17	2.0
18	10.0
19	30.0
20	59.0
21	131.0
22	207.0
23	397.0
24	647.0
25	962.0
26	1544.0
27	2327.0
28	3239.0
29	4167.0
30	5193.0
31	6118.0
32	7241.0
33	8903.0
34	11210.0
35	13648.0
36	18529.0
37	34360.0
38	33786.0
39	32820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.56963321441275	19.35944556419017	15.08046001789198	14.990461203505104
2	16.624990568986515	27.332154905743757	32.84148352536673	23.201370999902995
3	23.746214121730134	30.135052113085937	31.636469459683763	14.482264305500166
4	12.587438967869884	18.354907899416894	28.563575809180957	40.49407732353226
5	9.807176192888477	46.3725627566583	29.04536586943166	14.774895181021567
6	28.345854126472585	30.4497785059119	19.362679054527426	21.841688313088092
7	22.37629204884726	38.64991000118561	20.742840513478267	18.230957436488858
8	35.43258711561884	28.441242091421547	20.02069433815842	16.10547645480119
9	28.862134750320656	10.678062923721964	23.224005432263766	37.23579689369362
10	21.281216654630896	33.42297287101607	24.755602022009292	20.54020845234374
11	26.803479235602882	26.366419125017515	18.859332392028367	27.970769247351235
12	19.181603595641256	36.910831114799684	27.292814106640513	16.61475118291855
13	31.29964754955324	20.616734390325398	30.655644057383675	17.427974002737688
14	22.885027861908405	25.258948684508347	33.07483374470516	18.781189708878088
15	27.575744511150152	25.892712790609945	30.153375224997035	16.378167473242865
16	19.29208118216407	25.54134017396178	34.31972752454758	20.84685111932657
17	15.734702896129512	33.38417098696904	32.62106726737732	18.26005884952414
18	14.995311439010983	23.975791935675097	38.34218950409036	22.686707121223552
19	15.859192274113754	28.81740480065532	40.95484969659082	14.368553228640103
20	17.806292372196296	24.63973528492439	42.49345218206707	15.060520160812253
21	20.463682514362088	25.514394421151337	39.23571066728462	14.786212397201954
22	19.520042250940406	27.414069994287498	38.303926535099535	14.761961219672557
23	17.4166567865573	27.977775143081946	38.53835458455038	16.067213485810367
24	15.456622727125751	29.701764407894025	39.124694165705606	15.71691869927462
25	15.081537848004398	29.710925963849576	39.82258916349605	15.384947024649975
26	16.626068399098934	29.533622910356872	38.684400564782976	15.155908125761217
27	14.733937636749694	31.764731243061465	38.943618706819436	14.557712413369405
28	14.358313842572134	30.93641880166848	40.51293935049957	14.19232800525981
29	13.767124025911038	31.77227605384839	39.71642289742291	14.744177022817665
30	13.794608693777688	32.38879487815131	40.93059851906142	12.88599790900958
31	13.204496707229008	33.75602237575313	38.413865206566136	14.62561571045172
32	13.013720777331079	34.06320395779217	39.703488936073896	13.219586328802855
33	12.900009700471013	33.685424503389775	39.84145119046336	13.573114605675855
34	12.795460179566497	34.84463078929499	37.37914829864516	14.980760732493344
35	12.286724366505352	34.122484613975146	39.36936160122442	14.22142941829509
36	13.744489593550266	34.53313788680628	35.9176106662068	15.804761853436661
37	12.541092273035925	35.18360835965035	36.647301652313566	15.627997715000161
38	12.909171256426562	33.62883842248785	37.14579807930674	16.31619224177885
39	13.544013192640575	34.20008838206922	36.399939641513704	15.855958783776499
40	12.495284493258172	33.97805537891118	37.94447019260824	15.58218993522241
41	12.842345789456665	34.85540909041917	35.31240905808426	16.989836062039902
42	12.785759708554737	34.175837204539825	36.418262753424806	16.620140333480638
43	12.30989771392233	34.51104236950172	35.65354228866446	17.525517627911487
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16.0
1	43.0
2	70.0
3	371.0
4	672.0
5	672.0
6	1033.5
7	1395.0
8	1581.5
9	1768.0
10	2594.5
11	3421.0
12	3421.0
13	5939.0
14	8457.0
15	12695.0
16	16933.0
17	15082.5
18	13232.0
19	13232.0
20	13969.5
21	14707.0
22	10516.5
23	6326.0
24	5644.5
25	4963.0
26	4963.0
27	5317.0
28	5671.0
29	5720.0
30	5769.0
31	5885.0
32	6001.0
33	6001.0
34	5618.0
35	5235.0
36	5065.5
37	4896.0
38	5422.5
39	5949.0
40	5949.0
41	6462.5
42	6976.0
43	7501.0
44	8026.0
45	8771.5
46	9517.0
47	9517.0
48	10109.0
49	10701.0
50	9426.5
51	8152.0
52	6936.5
53	5721.0
54	5721.0
55	6350.0
56	6979.0
57	5551.0
58	4123.0
59	4928.5
60	5734.0
61	5734.0
62	5072.5
63	4411.0
64	3569.0
65	2727.0
66	2462.0
67	2197.0
68	2197.0
69	1954.5
70	1712.0
71	1449.0
72	1186.0
73	1006.0
74	826.0
75	826.0
76	678.5
77	531.0
78	404.0
79	277.0
80	219.5
81	162.0
82	162.0
83	119.5
84	77.0
85	49.5
86	22.0
87	20.0
88	18.0
89	18.0
90	10.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	185558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.925263260004954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.18920885258983	37.74561053686718
2	9.38299420310263	8.618329578891776
3	2.9641625008800956	4.0838982959505925
4	1.3952451360041305	2.5630800073292446
5	0.8566265343002653	1.9670399551622673
6	0.5855570419394963	1.613511678289268
7	0.38958905395573706	1.2524385906293451
8	0.32974254265530756	1.211481046357473
9	0.22765143514281022	0.9409456881406353
>10	1.4679997183693587	12.427920111232067
>50	0.11969302260085898	3.738453744920726
>100	0.07510150437700956	6.678235376539949
>500	0.009387688047126195	3.0637320945472575
>1k	0.0035203830176723226	3.2361849125340862
>5k	0.0035203830176723226	10.859138382608133
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7573	4.081203720669548	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7302	3.9351577404369524	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5275	2.842776921501633	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3159	1.7024326625637267	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	1487	0.8013666885825456	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	1359	0.7323855613878141	TruSeq Adapter, Index 10 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	899	0.4844846355317475	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	838	0.4516108171030082	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	792	0.4268207245174016	No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	724	0.39017450069520043	No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	702	0.378318369458606	No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	621	0.3346662499056898	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	570	0.307181582039039	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	539	0.29047521529656495	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	480	0.25867922698024337	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	480	0.25867922698024337	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	448	0.24143394518156047	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	406	0.21879951282078916	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	383	0.20640446652798586	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	378	0.20370989124694167	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	366	0.1972429105724356	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.18484786427963223	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	334	0.17999762877375267	No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA	329	0.17730305349270847	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	328	0.17676413843649963	RNA PCR Primer, Index 10 (95% over 22bp)
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	304	0.16383017708748748	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	287	0.15466862113193716	No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	248	0.1336509339397924	No Hit
GAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA	229	0.12341154787182443	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	226	0.12179480270319792	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	223	0.12017805753457139	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	222	0.11963914247836256	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	218	0.11748348225352719	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	213	0.11478890697248299	No Hit
GTACATGGGAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAA	205	0.11047758652281228	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	198	0.1067051811293504	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	190	0.10239386067967966	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.389150562088404E-4	0.0
2	0.0	0.0	0.0	0.0026945752810442016	0.0
3	0.0	0.0	0.0	0.004311320449670723	0.0
4	0.0	0.0	0.0	0.005389150562088403	0.0
5	0.0	0.0	0.0	0.005389150562088403	0.0
6	0.0	0.0	0.0	0.005389150562088403	0.0
7	0.0	0.0	0.0	0.005928065618297244	0.0
8	0.0	0.0	0.0	0.0064669806745060844	0.0
9	0.0	0.0	0.0	0.008083725843132605	0.0
10	0.0	0.0	0.0	0.011317216180385647	0.0
11	0.0	0.0	0.0	0.01347287640522101	0.0
12	0.0	0.0	0.0	0.01670636674247405	0.0
13	0.0	0.0	0.0	0.017784196854891732	0.0
14	0.0	0.0	0.0	0.023173347416980135	0.0
15	0.0	0.0	0.0	0.03017924314769506	0.0
16	0.0	0.0	0.0	0.043113204496707226	0.0
17	0.0	5.389150562088404E-4	0.0	0.06952004225094041	0.0
18	0.0	0.0010778301124176807	0.0	0.07760376809407302	0.0
19	0.0	0.0010778301124176807	0.0	0.09377121978033823	0.0
20	0.0	0.0010778301124176807	0.0	0.10993867146660344	0.0
21	0.0	0.0016167451686265211	0.0	0.14119574472671617	0.0
22	0.0	0.0016167451686265211	0.0	0.20370989124694167	0.0
23	0.0	0.0016167451686265211	0.0	0.27808016900376165	0.0
24	0.0	0.0016167451686265211	0.0	0.41334784811218056	0.0
25	0.0	0.0016167451686265211	0.0	0.44298817620366676	0.0
26	0.0	0.0016167451686265211	0.0	0.4871792108127917	0.0
27	0.0	0.0016167451686265211	0.0	0.5313702454219166	0.0
28	0.0	0.0016167451686265211	0.0	0.5971178822793951	0.0
29	0.0	0.0016167451686265211	0.0	0.6962782526218217	0.0
30	0.0	0.0016167451686265211	0.0	0.8978324836439281	0.0
31	0.0	0.0016167451686265211	0.0	1.3801614589508402	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCAAG	25	0.005484619	29.6	19
ATAAGCC	25	0.005484619	29.6	3
TAAGCCT	25	0.005484619	29.6	4
TCAACTG	25	0.005484619	29.6	32
GTTATAC	25	0.005484619	29.6	3
CGTCTTC	45	3.984489E-6	28.777777	37
GCCGTCT	180	0.0	26.722221	36
CCGTCTT	185	0.0	26.000002	37
TTTAACC	40	0.0019251228	23.125002	37
GGGGAGC	50	2.6902868E-4	22.2	20
AGCTGTG	50	2.6902868E-4	22.2	11
TGCCGTC	225	0.0	21.377777	35
TATACAC	310	0.0	20.887096	3
ATGCCGT	240	0.0	20.8125	34
GCTGTGG	55	5.1207875E-4	20.181818	12
TATGCCG	260	0.0	19.923077	33
ATACACA	345	0.0	18.768116	4
TACACAT	355	0.0	18.760563	5
TGTGGTA	60	9.1970817E-4	18.5	14
TGTTCCC	130	6.8394E-10	18.5	5
>>END_MODULE