FastQCFastQC Report
Fri 10 Feb 2017
ERR1630825.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630825.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1660059
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG17600.10602032819315459No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC17030.10258671529144445No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA16620.10011692355512665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.021.1117651
GTACTAG450.003826514120.5555551
AAGACGG2200.016.8181825
GGTCTAG1107.8127414E-716.8181821
TCTTATA8800.015.97727337
GCTTAGG5800.015.9482761
TCTAGCG1403.475725E-815.85714228
TACCGTC1403.475725E-815.8571427
TAGACAG2003.6379788E-1215.7249995
TAGGACA6600.015.696974
TCGTTTA1302.5907866E-715.65384630
TTAGGAC6550.015.2519073
CTTAGGA6600.014.8560612
CGGTGAT1508.107236E-814.814
CGCAATA1508.107236E-814.836
GAACCGT1001.09382905E-414.7999996
TAGACCA1405.9989725E-714.5357144
TAAGACC1405.9989725E-714.5357144
CGCCTTA1458.9160494E-714.03448325
ATACCGT1854.9021764E-914.06
Produced by FastQC (version 0.11.5)