##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630825.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1660059
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0778321734348	33.0	31.0	34.0	30.0	34.0
2	32.26185153660201	34.0	31.0	34.0	30.0	34.0
3	32.36697611349958	34.0	31.0	34.0	30.0	34.0
4	35.93733234782619	37.0	35.0	37.0	35.0	37.0
5	35.889109965368704	37.0	35.0	37.0	35.0	37.0
6	35.96145859876064	37.0	35.0	37.0	35.0	37.0
7	35.90767918489644	37.0	35.0	37.0	35.0	37.0
8	35.89679764393916	37.0	35.0	37.0	35.0	37.0
9	37.63385939897317	39.0	37.0	39.0	35.0	39.0
10	37.53294551579191	39.0	37.0	39.0	35.0	39.0
11	37.63040169054232	39.0	37.0	39.0	35.0	39.0
12	37.562877584471394	39.0	37.0	39.0	35.0	39.0
13	37.59975157509462	39.0	37.0	39.0	35.0	39.0
14	38.925371327163674	40.0	38.0	41.0	36.0	41.0
15	38.9476072838375	40.0	38.0	41.0	36.0	41.0
16	38.91233444112529	40.0	38.0	41.0	35.0	41.0
17	38.87331414124438	40.0	38.0	41.0	35.0	41.0
18	38.84810961538114	40.0	38.0	41.0	35.0	41.0
19	38.90200890450279	40.0	38.0	41.0	35.0	41.0
20	38.86465601523801	40.0	38.0	41.0	35.0	41.0
21	38.82155995660395	40.0	38.0	41.0	35.0	41.0
22	38.76188195720754	40.0	38.0	41.0	35.0	41.0
23	38.71143134069331	40.0	38.0	41.0	34.0	41.0
24	38.66214453823629	40.0	38.0	41.0	34.0	41.0
25	38.611584287064495	40.0	38.0	41.0	34.0	41.0
26	38.500623773010474	40.0	38.0	41.0	34.0	41.0
27	38.37771247889382	40.0	38.0	41.0	34.0	41.0
28	38.28841625508491	40.0	38.0	41.0	34.0	41.0
29	38.2292840194234	40.0	38.0	41.0	34.0	41.0
30	38.163247209888326	40.0	38.0	41.0	33.0	41.0
31	38.151275346237696	40.0	38.0	41.0	33.0	41.0
32	38.07242091997935	40.0	38.0	41.0	33.0	41.0
33	38.013521808562224	40.0	38.0	41.0	33.0	41.0
34	37.970332982140995	40.0	37.0	41.0	33.0	41.0
35	37.928070628815	40.0	37.0	41.0	33.0	41.0
36	37.91727462698615	40.0	37.0	41.0	33.0	41.0
37	37.814050584949086	40.0	37.0	41.0	33.0	41.0
38	37.689712835507656	40.0	37.0	41.0	33.0	41.0
39	37.6547725110975	40.0	37.0	41.0	33.0	41.0
40	37.51519554425475	40.0	37.0	41.0	32.0	41.0
41	37.48454301925413	40.0	37.0	41.0	32.0	41.0
42	37.38543750553444	40.0	36.0	41.0	32.0	41.0
43	36.54808413435908	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	4.0
11	3.0
12	1.0
13	3.0
14	2.0
15	2.0
16	4.0
17	16.0
18	47.0
19	97.0
20	160.0
21	355.0
22	651.0
23	1216.0
24	2046.0
25	3169.0
26	4843.0
27	7491.0
28	10694.0
29	15407.0
30	20919.0
31	28009.0
32	36863.0
33	48158.0
34	64319.0
35	88204.0
36	124725.0
37	191365.0
38	375432.0
39	635849.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96494341466177	17.61642206692654	11.544107769663608	26.874526748748085
2	19.738153884892043	19.789176167835	33.537603181573665	26.93506676569929
3	20.687096061043615	21.651158181727276	28.58302024205164	29.078725515177474
4	15.198736912362754	14.616890122579981	33.060873137641494	37.12349982741577
5	15.393609504240512	35.72035692707307	33.910782689049	14.97525087963741
6	37.33186591560902	33.295382874946014	14.680080647736013	14.692670561708951
7	30.922575643395806	28.96692225999196	20.233256769789506	19.87724532682272
8	28.05315955637721	32.75485991762944	19.22564198019468	19.966338545798674
9	27.2222854729862	13.329586478552871	18.08815228856324	41.35997575989769
10	17.354262709939828	25.85311726872358	32.52420546498648	24.268414556350105
11	37.61053071005308	20.427526973438894	20.20145067133156	21.760491645176465
12	22.650460013770594	24.083059698480596	27.828769941309318	25.437710346439495
13	31.389004848622847	18.645301160982832	23.997219375937842	25.968474614456472
14	24.05004882356591	19.47629572201952	23.283449564141996	33.19020589027257
15	26.90127278608772	26.374785474492175	20.848114434486966	25.875827304933136
16	27.143734048006728	25.08971066691003	22.01265135757223	25.75390392751101
17	25.495117944603173	25.086397531653997	23.60373938516643	25.8147451385764
18	25.297414128052075	23.927643535561085	24.873573770570808	25.901368565816036
19	26.20810465170214	24.044747807156252	24.235403681435418	25.511743859706193
20	26.474962636870135	23.4115173014935	24.39184390434316	25.721676157293206
21	26.364303919318534	24.02739902617919	23.544645099963315	26.06365195453896
22	26.617909363462385	23.696145739398418	23.675604300810996	26.010340596328202
23	25.91359704685195	24.060891811676573	23.98601495489016	26.039496186581317
24	26.09069918599279	24.403771191264887	23.71789195444258	25.78763766829974
25	26.557068152396994	23.922342519151428	23.703675592253045	25.816913736198533
26	26.249789917105353	24.479250436279674	23.62759395900989	25.64336568760508
27	26.726459722214692	23.554403789262913	23.551933997526593	26.1672024909958
28	25.79010746003606	23.843248944766422	24.14787667185323	26.21876692334429
29	25.26777662721626	24.17823703856309	24.431902721529777	26.122083612690872
30	25.281450840000264	25.073024512984176	24.119082514537133	25.526442132478422
31	26.114734476304758	24.486720050311465	23.572957346696715	25.825588126687066
32	25.30897998203678	24.19673035717405	24.09757725478432	26.396712406004845
33	25.379338927110425	23.88529564310666	24.333833917951107	26.40153151183181
34	26.056423295798524	23.639461007108782	24.344194995479075	25.959920701613616
35	25.855225627522877	23.80409370992236	24.852851615514872	25.487829047039895
36	25.246693039223306	24.427565526285512	24.353953684778674	25.97178774971251
37	26.386050134362694	23.217006142552766	24.47123867284235	25.925705050242193
38	25.32114822424986	23.474346393712512	25.199947712701775	26.00455766933585
39	25.49108194347309	23.464226271475894	25.044531549782267	26.000160235268748
40	24.878633831689115	23.130081521198946	25.87859829078364	26.1126863563283
41	24.33046054387224	23.230198444754073	26.44616847955404	25.993172531819653
42	25.06910898949977	22.943943558632554	26.143769588912203	25.843177862955475
43	24.39178366552032	22.52504278462392	26.392495688406253	26.6906778614495
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	26.5
2	29.0
3	86.5
4	144.0
5	144.0
6	206.0
7	268.0
8	277.5
9	287.0
10	389.0
11	491.0
12	491.0
13	875.0
14	1259.0
15	1913.0
16	2567.0
17	2797.0
18	3027.0
19	3027.0
20	4011.5
21	4996.0
22	6031.0
23	7066.0
24	8895.5
25	10725.0
26	10725.0
27	12967.5
28	15210.0
29	20068.0
30	24926.0
31	29703.5
32	34481.0
33	34481.0
34	39783.5
35	45086.0
36	51503.0
37	57920.0
38	66865.0
39	75810.0
40	75810.0
41	81557.5
42	87305.0
43	89426.0
44	91547.0
45	98379.0
46	105211.0
47	105211.0
48	110919.5
49	116628.0
50	122297.0
51	127966.0
52	137396.5
53	146827.0
54	146827.0
55	138277.0
56	129727.0
57	125862.5
58	121998.0
59	114061.5
60	106125.0
61	106125.0
62	100623.0
63	95121.0
64	85837.0
65	76553.0
66	67569.0
67	58585.0
68	58585.0
69	49772.0
70	40959.0
71	35725.5
72	30492.0
73	24136.5
74	17781.0
75	17781.0
76	13918.5
77	10056.0
78	8174.0
79	6292.0
80	4832.0
81	3372.0
82	3372.0
83	2501.0
84	1630.0
85	1239.5
86	849.0
87	653.0
88	457.0
89	457.0
90	336.0
91	215.0
92	123.5
93	32.0
94	21.0
95	10.0
96	10.0
97	7.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1660059.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.41764433238258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.40200660059692	49.88984046865461
2	8.76311159138908	10.23840672381493
3	2.3089754640541416	4.046547222939386
4	1.0053279087516187	2.3491555284348014
5	0.5732830863194541	1.674492371919023
6	0.36077425697242	1.2645349336856657
7	0.24595110402386017	1.0057518882615886
8	0.17683021085674505	0.8264003492039639
9	0.1422079927982788	0.7476710350060687
>10	0.8163192963547474	9.254450171950705
>50	0.10046008684115956	4.147621508395302
>100	0.09654907862283661	10.985070827357351
>500	0.007151614416419956	2.7722191542874546
>1k	0.0010517080024146995	0.7978378160892
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1760	0.10602032819315459	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1703	0.10258671529144445	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1662	0.10011692355512665	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.035823425552947E-4	0.0
3	0.0	0.0	0.0	0.001566209393762511	0.0
4	0.0	0.0	0.0	0.0031926576103620415	0.0
5	0.0	0.0	0.0	0.003915523484406277	0.0
6	0.0	0.0	0.0	0.004156478775754356	0.0
7	0.0	0.0	0.0	0.0046986281812875326	0.0
8	0.0	0.0	0.0	0.0052407775868207095	0.0
9	0.0	0.0	0.0	0.00867439048853083	0.0
10	0.0	0.0	0.0	0.012348958681589028	0.0
11	0.0	0.0	0.0	0.0137946904296775	0.0
12	0.0	0.0	0.0	0.014878989240743852	0.0
13	0.0	0.0	0.0	0.015782571583299147	0.0
14	0.0	0.0	0.0	0.016746392748691462	0.0
15	0.0	0.0	0.0	0.018372840965290994	0.0
16	0.0	0.0	0.0	0.02048119976458668	0.0
17	0.0	0.0	0.0	0.02222812562686025	0.0
18	0.0	0.0	0.0	0.02379433502062276	0.0
19	0.0	0.0	0.0	0.025059350300200175	0.0
20	0.0	0.0	0.0	0.026746037339636725	0.0
21	0.0	0.0	0.0	0.02879415731609539	0.0
22	0.0	0.0	0.0	0.031745859635109355	0.0
23	0.0	0.0	0.0	0.03698663722193007	0.0
24	0.0	0.0	0.0	0.043010519505632026	0.0
25	0.0	0.0	0.0	0.045118878304927716	0.0
26	0.0	0.0	0.0	0.048371774738126774	0.0
27	0.0	0.0	0.0	0.054696851136013844	0.0
28	0.0	0.0	0.0	0.07282873680995676	0.0
29	0.0	0.0	0.0	0.10276743175995552	0.0
30	0.0	0.0	0.0	0.1443924583403361	0.0
31	0.0	0.0	0.0	0.2933028283934487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	850	0.0	21.111765	1
GTACTAG	45	0.0038265141	20.555555	1
AAGACGG	220	0.0	16.818182	5
GGTCTAG	110	7.8127414E-7	16.818182	1
TCTTATA	880	0.0	15.977273	37
GCTTAGG	580	0.0	15.948276	1
TCTAGCG	140	3.475725E-8	15.857142	28
TACCGTC	140	3.475725E-8	15.857142	7
TAGACAG	200	3.6379788E-12	15.724999	5
TAGGACA	660	0.0	15.69697	4
TCGTTTA	130	2.5907866E-7	15.653846	30
TTAGGAC	655	0.0	15.251907	3
CTTAGGA	660	0.0	14.856061	2
CGGTGAT	150	8.107236E-8	14.8	14
CGCAATA	150	8.107236E-8	14.8	36
GAACCGT	100	1.09382905E-4	14.799999	6
TAGACCA	140	5.9989725E-7	14.535714	4
TAAGACC	140	5.9989725E-7	14.535714	4
CGCCTTA	145	8.9160494E-7	14.034483	25
ATACCGT	185	4.9021764E-9	14.0	6
>>END_MODULE