FastQCFastQC Report
Fri 10 Feb 2017
ERR1630824.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630824.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059018
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39280.37090965403798615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37570.3547626197099577No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30880.2915908889178465No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.16250904139495267No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC16080.15183877894426726No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT14090.13304778577890083No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA12880.12162210651754739No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA12730.1202056999975449No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTA358.8689313E-426.42857225
TGCGTTA957.1431714E-921.42105337
CAGATCG500.007034526718.4999989
CAGTCGG1750.017.9714310
ATGCGGC1552.0008883E-1117.90322735
AAGACGG1451.546141E-1017.8620685
CTCTATG2300.017.6956521
GGTATCA17650.017.504251
TCGGTGA1705.456968E-1217.41176413
AGTCGGT1603.45608E-1117.3437511
GCAGTCG1950.017.0769239
AATGCGT1201.0409349E-716.95833235
ATGCGTT1201.0409349E-716.95833236
TGTACTG2450.016.6122465
GTATCTA5350.016.59813126
TAGACTG904.4454257E-516.4444455
AAATGCG1352.2186214E-816.44444334
TATACTG1708.54925E-1116.3235285
CGTTTAG1251.6572267E-716.2826
ACCTAGG5200.016.0096151
Produced by FastQC (version 0.11.5)