##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630824.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1059018
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.335230373799124	31.0	31.0	34.0	30.0	34.0
2	31.524602981252443	31.0	31.0	34.0	30.0	34.0
3	31.622272709245735	31.0	31.0	34.0	30.0	34.0
4	35.37599266490277	37.0	35.0	37.0	33.0	37.0
5	35.14062744920294	37.0	35.0	37.0	32.0	37.0
6	35.220617591013564	37.0	35.0	37.0	32.0	37.0
7	35.17511222660993	37.0	35.0	37.0	32.0	37.0
8	35.16311337484349	37.0	35.0	37.0	32.0	37.0
9	36.7669879076654	38.0	37.0	39.0	33.0	39.0
10	36.54808039145699	38.0	35.0	39.0	32.0	39.0
11	36.70199373381755	38.0	35.0	39.0	32.0	39.0
12	36.6075411371667	38.0	35.0	39.0	32.0	39.0
13	36.70001170896057	38.0	35.0	39.0	32.0	39.0
14	37.79174669363505	39.0	37.0	40.0	33.0	41.0
15	37.77571674891267	39.0	37.0	40.0	33.0	41.0
16	37.71496707326976	39.0	37.0	40.0	33.0	41.0
17	37.67149944571292	39.0	37.0	40.0	33.0	41.0
18	37.641300714435445	39.0	37.0	40.0	33.0	41.0
19	37.685587969231875	39.0	37.0	40.0	32.0	41.0
20	37.64780390890429	39.0	37.0	40.0	32.0	41.0
21	37.58944418319613	39.0	37.0	40.0	32.0	41.0
22	37.529549072820295	39.0	37.0	40.0	32.0	41.0
23	37.45691008084849	39.0	36.0	40.0	32.0	41.0
24	37.379993541186266	39.0	36.0	40.0	32.0	41.0
25	37.3154082366872	39.0	36.0	40.0	32.0	41.0
26	37.131219677097086	39.0	36.0	40.0	31.0	41.0
27	36.96196476358287	39.0	36.0	40.0	31.0	41.0
28	36.84834724244536	39.0	36.0	40.0	31.0	41.0
29	36.74368802041136	39.0	36.0	40.0	30.0	41.0
30	36.62854833440036	38.0	35.0	40.0	30.0	41.0
31	36.574560583483944	38.0	35.0	40.0	30.0	41.0
32	36.47325069073425	38.0	35.0	40.0	30.0	41.0
33	36.37320045551634	38.0	35.0	40.0	30.0	41.0
34	36.30727145336529	38.0	35.0	40.0	30.0	41.0
35	36.24350577610579	38.0	35.0	40.0	30.0	41.0
36	36.214196548122885	38.0	35.0	40.0	30.0	41.0
37	36.04315601812245	38.0	35.0	40.0	29.0	41.0
38	35.878543140909784	38.0	35.0	40.0	29.0	41.0
39	35.81420334687418	38.0	35.0	40.0	29.0	41.0
40	35.613212428872785	38.0	34.0	40.0	28.0	41.0
41	35.543681032805864	38.0	34.0	40.0	27.0	41.0
42	35.38933427005018	38.0	34.0	40.0	27.0	41.0
43	34.500777135043975	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	0.0
12	2.0
13	5.0
14	2.0
15	2.0
16	19.0
17	44.0
18	75.0
19	159.0
20	326.0
21	612.0
22	1080.0
23	1815.0
24	2912.0
25	4590.0
26	6792.0
27	9782.0
28	13686.0
29	18248.0
30	23895.0
31	30509.0
32	39359.0
33	50325.0
34	65650.0
35	90262.0
36	131163.0
37	196770.0
38	253709.0
39	117220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.783297356607726	19.492019965666305	12.166743152618746	24.557939525107223
2	19.786538094725493	21.33995833876289	32.993679049836736	25.879824516674883
3	21.55496884849927	23.292710794339662	28.906213114413543	26.246107242747524
4	15.383874495051074	15.231658007701473	33.937950063171726	35.446517434075716
5	14.789644746359363	36.84441624221685	34.037948363483906	14.327990647939885
6	35.92280773320189	34.408669163319225	14.92070956301026	14.747813540468623
7	29.624425647156137	30.148023924050392	21.0423241153597	19.185226313433766
8	28.687331093522488	31.936662077509542	19.689467034554653	19.686539794413317
9	27.148830331495784	13.41478615094361	19.670392760085285	39.76599075747532
10	17.56476282744958	26.76640057109511	32.402470968387696	23.266365633067615
11	36.341025365007965	21.507377589427186	21.103701731226476	21.047895314338376
12	22.984406308485784	25.089847386918823	28.30848956297249	23.6172567416229
13	31.375387387183224	19.410907085620828	24.63159266414735	24.582112863048597
14	23.285723188840983	20.448377647971988	25.10958265109753	31.1563165120895
15	26.26215984997422	27.5195511313311	22.15476979616966	24.063519222525017
16	25.596259931370387	25.919295045032285	23.964937328732844	24.519507694864487
17	24.20761497915994	25.972174221779042	25.21968465125239	24.60052614780863
18	24.00582426361025	24.594954948829955	26.84364193998591	24.555578847573884
19	25.168788443633634	24.68928762306212	26.887550542105988	23.25437339119826
20	25.70164057645857	24.109599647975767	26.014571990277783	24.174187785287877
21	25.52846127261293	24.447176535243027	26.0785935649819	23.945768627162145
22	25.4728437099275	24.623094225027337	26.246768232456862	23.6572938325883
23	24.91921761480919	24.501944254016454	25.967075158307036	24.611762972867314
24	24.51554175660848	25.016194247878694	26.284255791686263	24.184008203826565
25	24.81988030420635	24.900709902947828	25.899559780853583	24.37985001199224
26	24.676823245686098	25.31345076287655	25.909946762000267	24.099779229437083
27	24.874459168777115	24.49420123170711	26.032890847936486	24.598448751579294
28	24.53357733296318	24.905997820622503	26.134872117376663	24.425552729037655
29	24.342740161168177	25.416093022026065	26.51824614879067	23.722920668015085
30	24.66417001410741	25.286727893199174	25.861883367421516	24.187218725271904
31	24.042461978927648	25.793046010549396	26.114664717691294	24.04982729283166
32	23.867299705954007	25.11156562022553	26.056686477472525	24.964448196347938
33	23.49818416684136	25.55272903765564	26.480097599851938	24.468989195651066
34	25.03281341771339	24.42763012526699	26.063957364275204	24.47559909274441
35	24.19760570641859	24.82337410695569	26.39775716748913	24.581263019136596
36	23.634348046964263	25.56708195705833	26.428445975422516	24.370124020554893
37	24.417809706728306	24.270314574445383	26.965736182010126	24.34613953681618
38	24.23764279738399	24.691931581899457	26.540247663401377	24.53017795731517
39	23.999308793618237	24.481453573027085	26.958559722308777	24.560677911045893
40	23.42462545490256	24.19930539424259	27.46336700603767	24.91270214481718
41	22.607170038658456	24.58079088362993	27.76808326204087	25.043955815670742
42	23.166745041160773	24.443021742784353	27.97610616627857	24.4141270497763
43	23.502433386401364	24.07381177657037	27.71888674224612	24.70486809478215
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	85.0
1	89.5
2	94.0
3	312.5
4	531.0
5	531.0
6	780.0
7	1029.0
8	1074.5
9	1120.0
10	1524.0
11	1928.0
12	1928.0
13	3332.0
14	4736.0
15	7072.5
16	9409.0
17	9043.0
18	8677.0
19	8677.0
20	10236.5
21	11796.0
22	11404.5
23	11013.0
24	12772.0
25	14531.0
26	14531.0
27	16380.5
28	18230.0
29	20977.5
30	23725.0
31	26754.0
32	29783.0
33	29783.0
34	33639.5
35	37496.0
36	41058.0
37	44620.0
38	48124.5
39	51629.0
40	51629.0
41	53567.0
42	55505.0
43	56768.5
44	58032.0
45	60083.5
46	62135.0
47	62135.0
48	64938.5
49	67742.0
50	68153.0
51	68564.0
52	69889.0
53	71214.0
54	71214.0
55	68846.5
56	66479.0
57	66209.0
58	65939.0
59	65632.0
60	65325.0
61	65325.0
62	61051.5
63	56778.0
64	52232.0
65	47686.0
66	41490.0
67	35294.0
68	35294.0
69	31827.0
70	28360.0
71	23664.0
72	18968.0
73	14097.5
74	9227.0
75	9227.0
76	7029.0
77	4831.0
78	3842.0
79	2853.0
80	2285.0
81	1717.0
82	1717.0
83	1308.5
84	900.0
85	701.5
86	503.0
87	416.5
88	330.0
89	330.0
90	245.5
91	161.0
92	91.5
93	22.0
94	16.0
95	10.0
96	10.0
97	10.0
98	10.0
99	5.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1059018.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.64477205809919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.31406449496487	57.09042568801274
2	7.380903244996382	9.542736157113515
3	1.7601467259594703	3.4135285166537863
4	0.7066716605503903	1.8273051366479374
5	0.39548288556800354	1.2782950495211456
6	0.24656960445533713	0.9563661525882567
7	0.1805036709550859	0.8168033065179165
8	0.12523883414288298	0.6476828708791008
9	0.09856821710801768	0.5734727934408932
>10	0.6371261180819532	8.009856837401053
>50	0.07576302343005706	3.472104647782954
>100	0.07339466032924258	8.819711527176919
>500	0.004394889045964871	1.8402531542672018
>1k	0.0011719704122572989	1.7114581619965454
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3928	0.37090965403798615	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3757	0.3547626197099577	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3088	0.2915908889178465	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1721	0.16250904139495267	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1608	0.15183877894426726	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1409	0.13304778577890083	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1288	0.12162210651754739	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1273	0.1202056999975449	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.001416406520002493	0.0
3	0.0	0.0	0.0	0.0026439588373379868	0.0
4	0.0	0.0	0.0	0.004154792458673979	0.0
5	0.0	9.442710133349953E-5	0.0	0.004626927965341476	0.0
6	0.0	9.442710133349953E-5	0.0	0.004721355066674976	0.0
7	0.0	9.442710133349953E-5	0.0	0.0052879176746759736	0.0
8	0.0	9.442710133349953E-5	0.0	0.005760053181343471	0.0
9	0.0	9.442710133349953E-5	0.0	0.010103699842684449	0.0
10	0.0	9.442710133349953E-5	0.0	0.014919482010692925	0.0
11	0.0	9.442710133349953E-5	0.0	0.016808024037362916	0.0
12	0.0	9.442710133349953E-5	0.0	0.01813000345603191	0.0
13	0.0	9.442710133349953E-5	0.0	0.019074274469366905	0.0
14	0.0	9.442710133349953E-5	0.0	0.0199241183813684	0.0
15	0.0	9.442710133349953E-5	0.0	0.021623806205371392	0.0
16	0.0	9.442710133349953E-5	0.0	0.024645473448043374	0.0
17	0.0	9.442710133349953E-5	0.0	0.028516984602716858	0.0
18	0.0	1.8885420266699906E-4	0.0	0.03031109952805335	0.0
19	0.0	1.8885420266699906E-4	0.0	0.03286063126405783	0.0
20	0.0	1.8885420266699906E-4	0.0	0.03578787140539632	0.0
21	0.0	1.8885420266699906E-4	0.0	0.040792507776071796	0.0
22	0.0	1.8885420266699906E-4	0.0	0.048535530085418756	0.0
23	0.0	2.832813040004986E-4	0.0	0.0593946467387712	0.0
24	0.0	2.832813040004986E-4	0.0	0.07469183715479813	0.0
25	0.0	2.832813040004986E-4	0.0	0.0799797548294741	0.0
26	0.0	2.832813040004986E-4	0.0	0.08687293322681956	0.0
27	0.0	2.832813040004986E-4	0.0	0.09754319567750501	0.0
28	0.0	3.777084053339981E-4	0.0	0.1205834084028789	0.0
29	0.0	4.721355066674976E-4	0.0	0.15589914430160773	0.0
30	0.0	4.721355066674976E-4	0.0	0.20934488365636844	0.0
31	0.0	4.721355066674976E-4	0.0	0.402259451680708	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTTA	35	8.8689313E-4	26.428572	25
TGCGTTA	95	7.1431714E-9	21.421053	37
CAGATCG	50	0.0070345267	18.499998	9
CAGTCGG	175	0.0	17.97143	10
ATGCGGC	155	2.0008883E-11	17.903227	35
AAGACGG	145	1.546141E-10	17.862068	5
CTCTATG	230	0.0	17.695652	1
GGTATCA	1765	0.0	17.50425	1
TCGGTGA	170	5.456968E-12	17.411764	13
AGTCGGT	160	3.45608E-11	17.34375	11
GCAGTCG	195	0.0	17.076923	9
AATGCGT	120	1.0409349E-7	16.958332	35
ATGCGTT	120	1.0409349E-7	16.958332	36
TGTACTG	245	0.0	16.612246	5
GTATCTA	535	0.0	16.598131	26
TAGACTG	90	4.4454257E-5	16.444445	5
AAATGCG	135	2.2186214E-8	16.444443	34
TATACTG	170	8.54925E-11	16.323528	5
CGTTTAG	125	1.6572267E-7	16.28	26
ACCTAGG	520	0.0	16.009615	1
>>END_MODULE