FastQCFastQC Report
Fri 10 Feb 2017
ERR1630823.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630823.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461764
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36670.25086128814227193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31200.21344074693315745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25530.1746519958078048No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG25330.17328378589156662No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT21320.14585117707099093No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG19730.13497390823689734No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA17560.12012883064571299No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17270.1181449262671676No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC17160.11739241081323662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG502.7025887E-422.25
GAGTACG2950.018.8135591
ACTAGCC500.007035773718.53
TACCGTC1306.9667294E-1018.57
GGTATCA17850.018.2408961
TAACGCA752.0677503E-417.2666669
CGTCGTA1201.0417352E-716.95833410
TCGCCAT4300.016.7790713
ATACCGT1251.6585E-716.2799996
GCATAGG803.3833514E-416.18751
TCTATAG1151.2426844E-616.0869563
AACCCGT1851.8189894E-1116.029
GTCGCCA4650.015.91397912
TAACCCG1751.3096724E-1015.85714328
AGTACGG3150.015.8571422
AGGACCG2350.015.7446795
ACCGTCG1302.5901682E-715.6538468
GTCTTAG1902.7284841E-1115.5789481
ACCCGTT1802.0190782E-1015.41666730
TCTATAC1455.3480107E-815.3103453
Produced by FastQC (version 0.11.5)