##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630823.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1461764
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11518754053322	33.0	31.0	34.0	30.0	34.0
2	32.300688756871835	34.0	31.0	34.0	30.0	34.0
3	32.39885576604705	34.0	31.0	34.0	30.0	34.0
4	35.965367870600176	37.0	35.0	37.0	35.0	37.0
5	35.91764060409204	37.0	35.0	37.0	35.0	37.0
6	35.98791118128508	37.0	35.0	37.0	35.0	37.0
7	35.93968725457734	37.0	35.0	37.0	35.0	37.0
8	35.93879518171196	37.0	35.0	37.0	35.0	37.0
9	37.658029613535426	39.0	37.0	39.0	35.0	39.0
10	37.57441967376403	39.0	37.0	39.0	35.0	39.0
11	37.65946486573756	39.0	37.0	39.0	35.0	39.0
12	37.61053973144776	39.0	37.0	39.0	35.0	39.0
13	37.6432440530756	39.0	37.0	39.0	35.0	39.0
14	38.97634159823337	40.0	38.0	41.0	36.0	41.0
15	38.993771908461284	40.0	38.0	41.0	36.0	41.0
16	38.95694722267069	40.0	38.0	41.0	36.0	41.0
17	38.94155691342789	40.0	38.0	41.0	36.0	41.0
18	38.91539879214429	40.0	38.0	41.0	35.0	41.0
19	38.983697094743064	40.0	38.0	41.0	35.0	41.0
20	38.95013695781262	40.0	38.0	41.0	35.0	41.0
21	38.90734961320706	40.0	38.0	41.0	35.0	41.0
22	38.851194857719854	40.0	38.0	41.0	35.0	41.0
23	38.80823922329459	40.0	38.0	41.0	35.0	41.0
24	38.76377855796148	40.0	38.0	41.0	35.0	41.0
25	38.719362359450635	40.0	38.0	41.0	35.0	41.0
26	38.60156632671211	40.0	38.0	41.0	34.0	41.0
27	38.47983258583465	40.0	38.0	41.0	34.0	41.0
28	38.408043979739546	40.0	38.0	41.0	34.0	41.0
29	38.34933067170898	40.0	38.0	41.0	34.0	41.0
30	38.28272210835675	40.0	38.0	41.0	34.0	41.0
31	38.25016076466516	40.0	38.0	41.0	34.0	41.0
32	38.16733891380551	40.0	38.0	41.0	34.0	41.0
33	38.111733494599676	40.0	38.0	41.0	33.0	41.0
34	38.0638304131173	40.0	38.0	41.0	33.0	41.0
35	38.02394709405896	40.0	38.0	41.0	33.0	41.0
36	38.00144004093684	40.0	37.0	41.0	33.0	41.0
37	37.89173081290824	40.0	37.0	41.0	33.0	41.0
38	37.75697239773315	40.0	37.0	41.0	33.0	41.0
39	37.71954569957941	40.0	37.0	41.0	33.0	41.0
40	37.58064639709283	40.0	37.0	41.0	33.0	41.0
41	37.536685812484095	40.0	37.0	41.0	32.0	41.0
42	37.432226405904096	40.0	37.0	41.0	32.0	41.0
43	36.66433364072449	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	2.0
12	0.0
13	0.0
14	3.0
15	5.0
16	2.0
17	19.0
18	38.0
19	74.0
20	141.0
21	291.0
22	501.0
23	978.0
24	1606.0
25	2593.0
26	4099.0
27	6211.0
28	9132.0
29	13071.0
30	17774.0
31	23419.0
32	31008.0
33	40815.0
34	54570.0
35	74653.0
36	105827.0
37	166694.0
38	326446.0
39	581785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13863250155292	19.072025306410612	12.288303720710045	25.501038471326424
2	18.441212124528995	21.208553501112355	35.1207171609097	25.229517213448954
3	20.197993656978827	23.36375776117075	29.953398770252925	26.484849811597496
4	14.84220435036025	15.491488366111083	34.17377907788124	35.492528205647424
5	14.326047159459392	37.16817489006433	34.33474897452667	14.171028975949607
6	35.02138512099081	34.29028215224893	15.815890937251156	14.872441789509114
7	28.75676237751101	30.761326725791577	21.50182929665801	18.980081600039405
8	26.659638628396927	34.17104265804877	20.235072145708884	18.934246567845424
9	26.51946552247832	14.499194124359335	19.704275108704277	39.277065244458065
10	16.858603714416283	27.29927676423828	33.17642245943942	22.665697061906027
11	35.24515585279156	21.988227921880686	21.6275677879603	21.139048437367457
12	21.174621895189645	25.874012494492955	29.01836411349575	23.93300149682165
13	29.620102834657303	20.206271326972068	25.522587777507177	24.65103806086345
14	22.789930522300452	21.15074663215129	24.92331183419485	31.136011011353403
15	25.431464997085712	28.05138175519441	22.703117603115142	23.81403564460474
16	25.155018183509785	26.780793616479816	23.89660711304971	24.167581086960684
17	23.75780221704735	26.83100692040576	25.284656073073354	24.126534789473542
18	22.954799817207157	25.604338320002405	27.024129750082775	24.41673211270766
19	24.246663620119254	25.800881674470023	26.919598512482178	23.032856192928545
20	24.43540817806431	25.0568491220197	27.117373255874412	23.390369444041585
21	24.146989527721303	26.043396882123243	25.96595620086416	23.843657389291295
22	24.338812557977896	25.357034377642357	26.027457236599066	24.27669582778068
23	23.63828908086394	26.1166645231378	26.16010518797836	24.0849412080199
24	24.660889172260365	26.055916002856822	25.87455977845945	23.408635046423363
25	24.487742207360423	25.378720504814726	26.267304434915623	23.866232852909224
26	24.347569101441817	25.867650318382445	26.005429056947634	23.7793515232281
27	24.40311842404109	25.344583667404585	25.929835459075473	24.32246244947885
28	23.591359480736973	25.57977895200593	26.613051080749013	24.215810486508083
29	23.646908803336242	26.222153507679764	26.329352754617023	23.80158493436697
30	23.244928729945464	26.99416595291716	26.223042844125317	23.53786247301206
31	24.094518677433566	26.009328455208912	26.126310403047277	23.769842464310244
32	23.23302530367419	26.145191699891363	26.254580082694606	24.36720291373984
33	23.40528293212858	25.74054361716392	26.515839766200287	24.338333684507212
34	23.7316694076472	25.66241883094672	26.498395089768252	24.10751667163783
35	23.975210772737597	25.720499341891028	26.47534075267964	23.828949132691733
36	23.506804107913453	26.23610924882539	26.352270270714012	23.904816372547142
37	24.35543630846019	25.133468877329037	26.288306457129877	24.222788357080898
38	23.587460082475694	25.60208077364062	27.03719615478285	23.77326298910084
39	23.5765828136416	25.442615907903054	27.029671000243543	23.951130278211803
40	23.335641047392055	24.9477343811997	27.667120000218915	24.04950457118933
41	22.62848175218435	25.65557778136553	27.747707564285346	23.968232902164782
42	23.38893282362953	24.86536814424216	27.606097837954692	24.139601194173615
43	22.683552201312935	24.921464750807928	27.60418234407196	24.790800703807182
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68.0
1	81.5
2	95.0
3	305.0
4	515.0
5	515.0
6	745.5
7	976.0
8	1006.5
9	1037.0
10	1565.5
11	2094.0
12	2094.0
13	3458.5
14	4823.0
15	7164.5
16	9506.0
17	9600.0
18	9694.0
19	9694.0
20	11207.0
21	12720.0
22	13652.0
23	14584.0
24	16525.0
25	18466.0
26	18466.0
27	21583.0
28	24700.0
29	32560.0
30	40420.0
31	42818.0
32	45216.0
33	45216.0
34	50028.0
35	54840.0
36	58765.0
37	62690.0
38	72549.5
39	82409.0
40	82409.0
41	88669.0
42	94929.0
43	90726.0
44	86523.0
45	91754.0
46	96985.0
47	96985.0
48	100967.0
49	104949.0
50	107718.5
51	110488.0
52	115354.0
53	120220.0
54	120220.0
55	107500.5
56	94781.0
57	88979.0
58	83177.0
59	76092.5
60	69008.0
61	69008.0
62	64355.5
63	59703.0
64	53367.0
65	47031.0
66	41641.5
67	36252.0
68	36252.0
69	31167.5
70	26083.0
71	22507.0
72	18931.0
73	15260.5
74	11590.0
75	11590.0
76	9202.0
77	6814.0
78	5598.0
79	4382.0
80	3414.5
81	2447.0
82	2447.0
83	1834.5
84	1222.0
85	960.0
86	698.0
87	569.5
88	441.0
89	441.0
90	328.5
91	216.0
92	125.5
93	35.0
94	20.0
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1461764.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.26343870175392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.89408631504529	49.18590746544756
2	8.487709175727527	9.720708282051751
3	2.2352859748899014	3.8400048421199435
4	0.9883775699268889	2.263915935587876
5	0.5235156538149148	1.4989153275819498
6	0.3392752804964257	1.1656841536636464
7	0.22787831021504518	0.9134366953920953
8	0.1766562516995008	0.8092755552380781
9	0.13050929037456102	0.672606967443577
>10	0.788354387025219	8.817174544710127
>50	0.0970880319608946	3.959039692465538
>100	0.10181236216664603	11.911106125153312
>500	0.006340810415551429	2.4204156943555932
>1k	0.003110586241591267	2.82180871878892
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3667	0.25086128814227193	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3120	0.21344074693315745	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2553	0.1746519958078048	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2533	0.17328378589156662	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2132	0.14585117707099093	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1973	0.13497390823689734	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1756	0.12012883064571299	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1727	0.1181449262671676	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1716	0.11739241081323662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.841049581190945E-5	0.0
2	0.0	0.0	0.0	2.0523148743572834E-4	0.0
3	0.0	0.0	0.0	2.736419832476378E-4	0.0
4	0.0	0.0	0.0	8.209259497429133E-4	0.0
5	0.0	0.0	0.0	9.577469413667323E-4	0.0
6	0.0	0.0	0.0	9.577469413667323E-4	0.0
7	0.0	0.0	0.0	0.0010261574371786418	0.0
8	0.0	0.0	0.0	0.0011629784288024606	0.0
9	0.0	0.0	0.0	0.0030100618157240158	0.0
10	0.0	0.0	0.0	0.003830987765466929	0.0
11	0.0	0.0	0.0	0.004446682227774114	0.0
12	0.0	0.0	0.0	0.0046519137152098424	0.0
13	0.0	0.0	0.0	0.004857145202645571	0.0
14	0.0	0.0	0.0	0.005267608177517027	0.0
15	0.0	0.0	0.0	0.005814892144012303	0.0
16	0.0	0.0	0.0	0.007046281068626673	0.0
17	0.0	0.0	0.0	0.0086881329681125	0.0
18	0.0	0.0	0.0	0.009235416934607775	0.0
19	0.0	0.0	0.0	0.009782700901103052	0.0
20	0.0	0.0	0.0	0.010808858338281692	0.0
21	0.0	0.0	0.0	0.012519120733579428	0.0
22	0.0	0.0	0.0	0.015323951061867716	0.0
23	0.0	1.368209916238189E-4	0.0	0.019360170314770372	0.0
24	0.0	1.368209916238189E-4	0.0	0.02613280940014941	0.0
25	0.0	1.368209916238189E-4	0.0	0.027979892787070963	0.0
26	0.0	1.368209916238189E-4	0.0	0.031468828073478346	0.0
27	0.0	1.368209916238189E-4	0.0	0.040088550545778937	0.0
28	0.0	1.368209916238189E-4	0.0	0.05787527945687539	0.0
29	0.0	2.0523148743572834E-4	0.0	0.08633404571462971	0.0
30	0.0	2.0523148743572834E-4	0.0	0.12628577526878484	0.0
31	0.0	2.0523148743572834E-4	0.0	0.26680093366644686	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACCG	50	2.7025887E-4	22.2	5
GAGTACG	295	0.0	18.813559	1
ACTAGCC	50	0.0070357737	18.5	3
TACCGTC	130	6.9667294E-10	18.5	7
GGTATCA	1785	0.0	18.240896	1
TAACGCA	75	2.0677503E-4	17.266666	9
CGTCGTA	120	1.0417352E-7	16.958334	10
TCGCCAT	430	0.0	16.77907	13
ATACCGT	125	1.6585E-7	16.279999	6
GCATAGG	80	3.3833514E-4	16.1875	1
TCTATAG	115	1.2426844E-6	16.086956	3
AACCCGT	185	1.8189894E-11	16.0	29
GTCGCCA	465	0.0	15.913979	12
TAACCCG	175	1.3096724E-10	15.857143	28
AGTACGG	315	0.0	15.857142	2
AGGACCG	235	0.0	15.744679	5
ACCGTCG	130	2.5901682E-7	15.653846	8
GTCTTAG	190	2.7284841E-11	15.578948	1
ACCCGTT	180	2.0190782E-10	15.416667	30
TCTATAC	145	5.3480107E-8	15.310345	3
>>END_MODULE