FastQCFastQC Report
Fri 10 Feb 2017
ERR1630822.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630822.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495873
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT181073.6515398095883422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT164053.308306764030306No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT148062.9858451660001655No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84331.700637058279035No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24080.4856082101667161No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21920.4420486697198678No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17360.35008963988763253No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15500.3125800356139576No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11570.2333258717453864No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10850.2188060249297703No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.1984379064800866No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT9820.19803457740187508No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9760.19682459016724038No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8140.1641549348321042No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7710.15548335965055568No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7500.1512484043293343No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6830.13773688020924713No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5870.11837708445509233No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA5790.1167637681422461No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA5600.1129321418992363No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT5130.10345390856126468Illumina PCR Primer Index 11 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC5120.10325224402215889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC250.00549336729.61
CCAATAC250.00549336729.63
GATACAC454.002217E-628.7777773
TACCCAG405.9334237E-527.755
GAGAGTG601.3348454E-624.6666669
ATACACT451.3217304E-424.6666664
GCCGTCT701.9183426E-723.78571336
CTAGATA400.001929647423.1253
CTAGTGT400.001929647423.12518
GGAGAGT603.7207894E-521.5833328
GCAGTCG1650.021.303039
GAACTGC450.003822786720.5555556
AGAGTAT450.003822786720.5555556
TATGTGC450.003822786720.5555554
CAGTCGG1650.020.18181810
GGCAGTC1900.019.4736848
AGTCGGT1750.019.02857211
GGTATCA67450.018.5959971
TCGCACG500.007029379718.522
CCAGGAC500.007029379718.53
Produced by FastQC (version 0.11.5)