##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630822.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495873
Sequences flagged as poor quality	0
Sequence length	43
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95155009447984	33.0	31.0	34.0	30.0	34.0
2	31.991380857598617	33.0	31.0	34.0	30.0	34.0
3	32.00337183109385	33.0	31.0	34.0	30.0	34.0
4	35.68302166078814	37.0	35.0	37.0	33.0	37.0
5	35.70521887660752	37.0	35.0	37.0	35.0	37.0
6	35.86339042456435	37.0	35.0	37.0	35.0	37.0
7	35.78125447443196	37.0	35.0	37.0	35.0	37.0
8	35.8567717943909	37.0	35.0	37.0	35.0	37.0
9	37.43143506502673	39.0	37.0	39.0	34.0	39.0
10	37.39547827770417	39.0	37.0	39.0	34.0	39.0
11	37.541939165875135	39.0	37.0	39.0	35.0	39.0
12	37.502384683174924	39.0	37.0	39.0	35.0	39.0
13	37.55272418542651	39.0	37.0	39.0	35.0	39.0
14	38.59442034553202	40.0	38.0	41.0	34.0	41.0
15	38.54390741177681	40.0	38.0	41.0	34.0	41.0
16	38.44977847150379	40.0	38.0	41.0	34.0	41.0
17	38.667854470802	40.0	38.0	41.0	35.0	41.0
18	38.75053693183537	40.0	38.0	41.0	35.0	41.0
19	38.89110114888288	40.0	38.0	41.0	35.0	41.0
20	38.90106136046931	40.0	38.0	41.0	35.0	41.0
21	38.84987285050809	40.0	38.0	41.0	35.0	41.0
22	38.69877569458309	40.0	38.0	41.0	35.0	41.0
23	38.56022812292664	40.0	38.0	41.0	34.0	41.0
24	38.43433903438985	40.0	38.0	41.0	34.0	41.0
25	38.32584956228712	40.0	37.0	41.0	34.0	41.0
26	38.00488431513714	40.0	37.0	41.0	34.0	41.0
27	37.73300018351473	40.0	35.0	41.0	33.0	41.0
28	37.53880529893743	40.0	35.0	41.0	33.0	41.0
29	37.317147737424705	39.0	35.0	41.0	33.0	41.0
30	36.98861805341287	39.0	35.0	41.0	33.0	41.0
31	36.61612549987598	38.0	35.0	40.0	32.0	41.0
32	36.23200900230503	38.0	35.0	40.0	31.0	41.0
33	35.86160166010249	37.0	35.0	40.0	30.0	41.0
34	35.573794096472284	37.0	35.0	40.0	30.0	41.0
35	35.236719885938534	37.0	35.0	40.0	28.0	41.0
36	34.90635102133006	37.0	35.0	40.0	25.0	41.0
37	34.45424735769038	36.0	35.0	40.0	22.0	41.0
38	33.987656113561336	35.0	34.0	40.0	20.0	41.0
39	33.557739582514074	35.0	33.0	40.0	16.0	41.0
40	32.98409673444612	35.0	33.0	40.0	12.0	41.0
41	32.501156546131774	35.0	33.0	40.0	10.0	41.0
42	31.918148396867746	35.0	31.0	40.0	8.0	41.0
43	31.057091231020845	35.0	30.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	10.0
12	10.0
13	3.0
14	5.0
15	6.0
16	13.0
17	6.0
18	26.0
19	57.0
20	142.0
21	299.0
22	537.0
23	913.0
24	1529.0
25	2371.0
26	3605.0
27	5376.0
28	7906.0
29	10804.0
30	13139.0
31	15953.0
32	18560.0
33	22834.0
34	28662.0
35	35153.0
36	47850.0
37	88947.0
38	91697.0
39	99451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.85359961119077	19.019184347605133	13.966075991231625	15.161140049972474
2	17.651092114311528	25.101991840652747	34.27732504088748	22.969591004148242
3	24.39435097293057	30.008086748018144	29.005612324123316	16.591949954927976
4	12.46569182028463	16.81136097347506	30.452152063129066	40.270795143111236
5	10.581136702341123	45.321685189554586	28.766841509822072	15.330336598282221
6	29.534780074736876	31.620394738168844	18.663044771544328	20.181780415549948
7	24.104962359313774	36.525884651916925	20.940039082587678	18.429113906181623
8	34.247277024560724	27.95655339169505	19.755461579880333	18.04070800386389
9	29.726563051426474	10.043902370163329	23.53102508101873	36.69850949739147
10	21.107823979123687	31.74683840418817	27.156751829601532	19.988585787086613
11	29.23470324054748	24.391124340304877	19.556015350704715	26.81815706844293
12	21.63033679994676	34.01334615919802	28.27619168617771	16.080125354677506
13	31.609706517596237	20.65992703776975	30.41282747800344	17.31753896663057
14	23.272692806424224	23.466290763965773	33.554156003654164	19.70686042595584
15	26.542683308024433	24.96627160583456	31.133374876228388	17.35767020991262
16	19.067583836990522	26.02400211344437	33.28614383118258	21.622270218382532
17	17.746681105847667	29.12479606673483	32.05377183270717	21.07475099471034
18	16.625426268419535	23.29346425395212	38.33058061237454	21.750528865253806
19	17.932010817285878	25.858838855916737	40.62733804825026	15.581812278547128
20	19.857503836667856	22.623332990503616	41.690513498415925	15.8286496744126
21	21.90419724405241	23.657065417959842	38.35962030600577	16.07911703198198
22	21.149165209640373	24.47844508573768	38.38341672162026	15.988972983001695
23	19.07464209585922	25.32805778899033	38.27310621872939	17.324193896421058
24	16.75509656706455	27.460055296416623	39.26811905467731	16.516729081841518
25	16.693185553559076	27.3055802594616	38.92246603465	17.078768152329328
26	17.17234049847441	27.778080274586436	38.567939774902044	16.481639452037115
27	16.530442270500714	28.499635995506917	38.701038370711856	16.268883363280516
28	15.68062790270896	28.487737787699675	39.428644027805504	16.402990281785858
29	15.360787943687193	29.15040746320126	39.986851472050304	15.501953121061241
30	14.75619765544807	30.703627743393973	39.19753646599028	15.342638135167675
31	15.657234816172688	30.95429676550246	37.64592950211042	15.742538916214436
32	14.442205968060371	31.086790367694956	38.5804429763266	15.890560687918075
33	14.147977405505038	31.07932877974804	38.43927779895255	16.333416015794366
34	15.156098436494828	30.975874871186775	38.1022963541068	15.765730338211597
35	14.382513264485059	31.793826241799817	37.52714908857712	16.29651140513801
36	14.550298161021068	32.31593573354468	36.01829500698768	17.115471098446577
37	14.507343614191537	32.158839057581275	36.177609992881244	17.156207335345947
38	14.382109935406847	31.614546466534776	36.017690013370355	17.985653584688016
39	14.570867944009859	32.01787554474634	36.34378157310441	17.0674749381394
40	13.957404416050077	31.85331728083602	36.40932254831378	17.77995575480012
41	13.973134250100328	32.270359547706775	35.66639038624809	18.090115815944806
42	14.037666902614177	32.45004265205002	35.73878795578707	17.773502489548733
43	13.532093903075989	31.939831368112397	34.7139287680515	19.81414596076011
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	289.0
1	291.5
2	294.0
3	1063.0
4	1832.0
5	1832.0
6	2952.5
7	4073.0
8	4370.0
9	4667.0
10	6723.5
11	8780.0
12	8780.0
13	15199.0
14	21618.0
15	30525.0
16	39432.0
17	36003.5
18	32575.0
19	32575.0
20	32457.0
21	32339.0
22	23247.5
23	14156.0
24	12628.0
25	11100.0
26	11100.0
27	11076.0
28	11052.0
29	11225.5
30	11399.0
31	11789.0
32	12179.0
33	12179.0
34	12380.5
35	12582.0
36	12958.5
37	13335.0
38	15047.0
39	16759.0
40	16759.0
41	18296.5
42	19834.0
43	21150.5
44	22467.0
45	24735.0
46	27003.0
47	27003.0
48	27717.0
49	28431.0
50	26738.5
51	25046.0
52	24878.5
53	24711.0
54	24711.0
55	22610.0
56	20509.0
57	19597.5
58	18686.0
59	17253.5
60	15821.0
61	15821.0
62	14258.0
63	12695.0
64	11337.0
65	9979.0
66	9003.0
67	8027.0
68	8027.0
69	6517.0
70	5007.0
71	4305.0
72	3603.0
73	2835.5
74	2068.0
75	2068.0
76	1744.5
77	1421.0
78	1211.0
79	1001.0
80	729.5
81	458.0
82	458.0
83	382.0
84	306.0
85	252.0
86	198.0
87	139.0
88	80.0
89	80.0
90	66.0
91	52.0
92	30.5
93	9.0
94	4.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	495873.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.1052376909934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.21161239363879	39.66808018144161
2	8.56033255063114	8.064729990228555
3	2.46381858072577	3.481762796177202
4	1.159080064784091	2.183949678181866
5	0.6725204262375694	1.5839617264984451
6	0.4647484612161641	1.3135252039266505
7	0.3467023108891647	1.1432046333715555
8	0.28273083436637675	1.065448252432085
9	0.19738351192814274	0.8368017521092376
>10	1.402440321170378	12.86295467835149
>50	0.1407748535384994	4.704334122173629
>100	0.0884134750878248	7.606913734167383
>500	0.0051502995196791145	1.7611179912382948
>1k	0.0025751497598395573	2.04759534097824
>5k	4.2919162663992614E-4	1.7049142486640498
>10k+	0.0012875748799197786	9.970705670059719
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18107	3.6515398095883422	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16405	3.308306764030306	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14806	2.9858451660001655	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8433	1.700637058279035	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2408	0.4856082101667161	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2192	0.4420486697198678	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1736	0.35008963988763253	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1550	0.3125800356139576	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1157	0.2333258717453864	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1085	0.2188060249297703	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	984	0.1984379064800866	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	982	0.19803457740187508	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	976	0.19682459016724038	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	814	0.1641549348321042	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	771	0.15548335965055568	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	750	0.1512484043293343	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	683	0.13773688020924713	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	587	0.11837708445509233	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	579	0.1167637681422461	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	560	0.1129321418992363	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	513	0.10345390856126468	Illumina PCR Primer Index 11 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	512	0.10325224402215889	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.016645391057791E-4	0.0
2	0.0	0.0	0.0	0.002621639008375128	0.0
3	0.0	0.0	0.0	0.005444942555856036	0.0
4	0.0	0.0	0.0	0.007461587946913826	0.0
5	0.0	0.0	0.0	0.008469910642442723	0.0
6	0.0	0.0	0.0	0.008671575181548501	0.0
7	0.0	0.0	0.0	0.009881562416183176	0.0
8	0.0	0.0	0.0	0.010284891494394734	0.0
9	0.0	0.0	0.0	0.018754802136837456	0.0
10	0.0	0.0	6.049936173173373E-4	0.027829706396597514	0.0
11	0.0	0.0	6.049936173173373E-4	0.03387964256977089	0.0
12	0.0	0.0	6.049936173173373E-4	0.04194622413400205	0.0
13	0.0	0.0	6.049936173173373E-4	0.04517285675969452	0.0
14	0.0	0.0	6.049936173173373E-4	0.05384443194124302	0.0
15	0.0	0.0	6.049936173173373E-4	0.06594430428758977	0.0
16	0.0	0.0	6.049936173173373E-4	0.10103393409199533	0.0
17	0.0	0.0	6.049936173173373E-4	0.1437868163824205	0.0
18	0.0	0.0	6.049936173173373E-4	0.1611299667455175	0.0
19	0.0	0.0	6.049936173173373E-4	0.1901696603767497	0.0
20	0.0	0.0	6.049936173173373E-4	0.20912612705269293	0.0
21	0.0	0.0	6.049936173173373E-4	0.2633738880721475	0.0
22	0.0	0.0	6.049936173173373E-4	0.3462580136446227	0.0
23	0.0	0.0	0.0010083226955288954	0.49750641797395706	0.0
24	0.0	0.0	0.0010083226955288954	0.7108675003478714	0.0
25	0.0	0.0	0.0010083226955288954	0.7598719833505756	0.0
26	0.0	0.0	0.0012099872346346746	0.8340845337415024	0.0
27	0.0	0.0	0.0012099872346346746	0.8838956749006298	0.0
28	0.0	0.0	0.0012099872346346746	0.9639564969256241	0.0
29	0.0	0.0	0.0012099872346346746	1.0879801884756783	0.0
30	0.0	0.0	0.0012099872346346746	1.3122311559613047	0.0
31	0.0	0.0	0.0012099872346346746	1.9256946839210847	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTAC	25	0.005493367	29.6	1
CCAATAC	25	0.005493367	29.6	3
GATACAC	45	4.002217E-6	28.777777	3
TACCCAG	40	5.9334237E-5	27.75	5
GAGAGTG	60	1.3348454E-6	24.666666	9
ATACACT	45	1.3217304E-4	24.666666	4
GCCGTCT	70	1.9183426E-7	23.785713	36
CTAGATA	40	0.0019296474	23.125	3
CTAGTGT	40	0.0019296474	23.125	18
GGAGAGT	60	3.7207894E-5	21.583332	8
GCAGTCG	165	0.0	21.30303	9
GAACTGC	45	0.0038227867	20.555555	6
AGAGTAT	45	0.0038227867	20.555555	6
TATGTGC	45	0.0038227867	20.555555	4
CAGTCGG	165	0.0	20.181818	10
GGCAGTC	190	0.0	19.473684	8
AGTCGGT	175	0.0	19.028572	11
GGTATCA	6745	0.0	18.595997	1
TCGCACG	50	0.0070293797	18.5	22
CCAGGAC	50	0.0070293797	18.5	3
>>END_MODULE