FastQCFastQC Report
Fri 10 Feb 2017
ERR1630821.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630821.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277345
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34391.2399718761830931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33021.1905749157186898No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22280.8033315906181832No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14930.5383187005354342No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT14070.5073103895869765No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT11820.426183994663686RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG7590.2736663722078999No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC7110.25635940795759793No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC6360.22931727631650112No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5020.18100200111774142No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG4040.14566694910670824No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3910.14097964628891813No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG3800.13701346698155725No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.13485009645026952No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG3690.1330472876741964No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3560.1283599848564063No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3530.1272782995907624No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.12006706448646992No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG3230.11646144693432368No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG3210.11574032342389443No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3200.1153797616686798No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT3170.11429807640303592No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.1139375146478213No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT3130.11285582938217745No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC3110.11213470587174817No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG2940.10600515603309955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC251.2312114E-437.037
GTAAGCC200.001839528837.09
AGGAGTG303.592501E-430.8333325
GCCGTCT1400.030.39285936
TTTAGCG250.005489243729.626
ATGCACC250.005489243729.64
CTAGGGG250.005489243729.618
CCGTCTT1450.029.34482837
GTACGGA451.3196781E-424.6666666
GTGTGAT551.895918E-523.5454543
CATCGTA400.001927513923.12500224
TATTAGC652.6706748E-622.769232
TACTTGT502.6949443E-422.216
CCTAGTG502.6949443E-422.217
AAGGTTT502.6949443E-422.27
TGTGATA502.6949443E-422.24
ATAGTAC705.078922E-621.1428593
GTATTAG1500.020.9666671
ACTTTTA806.921E-720.812536
GAGGCAC806.921E-720.81257
Produced by FastQC (version 0.11.5)