##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630821.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	277345
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.006219690277455	33.0	31.0	34.0	30.0	34.0
2	32.147696911788564	33.0	31.0	34.0	30.0	34.0
3	32.208772467504375	34.0	31.0	34.0	30.0	34.0
4	35.8283077034019	37.0	35.0	37.0	35.0	37.0
5	35.82307955795129	37.0	35.0	37.0	35.0	37.0
6	35.921534550830195	37.0	35.0	37.0	35.0	37.0
7	35.862150029746346	37.0	35.0	37.0	35.0	37.0
8	35.89321242495809	37.0	35.0	37.0	35.0	37.0
9	37.5590870576358	39.0	37.0	39.0	35.0	39.0
10	37.47195009825308	39.0	37.0	39.0	35.0	39.0
11	37.58846562945068	39.0	37.0	39.0	35.0	39.0
12	37.53575150083831	39.0	37.0	39.0	35.0	39.0
13	37.593711802989056	39.0	37.0	39.0	35.0	39.0
14	38.79662874758874	40.0	38.0	41.0	35.0	41.0
15	38.77516450630082	40.0	38.0	41.0	35.0	41.0
16	38.70558690439705	40.0	38.0	41.0	34.0	41.0
17	38.74658277596495	40.0	38.0	41.0	35.0	41.0
18	38.79277794804305	40.0	38.0	41.0	35.0	41.0
19	38.903156718166905	40.0	38.0	41.0	35.0	41.0
20	38.854607077827254	40.0	38.0	41.0	35.0	41.0
21	38.822466602967424	40.0	38.0	41.0	35.0	41.0
22	38.74475833348357	40.0	38.0	41.0	35.0	41.0
23	38.68568028989165	40.0	38.0	41.0	35.0	41.0
24	38.619178279759865	40.0	38.0	41.0	34.0	41.0
25	38.52881789828553	40.0	38.0	41.0	34.0	41.0
26	38.337125241125676	40.0	38.0	41.0	34.0	41.0
27	38.17416214462132	40.0	37.0	41.0	34.0	41.0
28	38.07507256305324	40.0	37.0	41.0	33.0	41.0
29	37.96616849050821	40.0	37.0	41.0	33.0	41.0
30	37.79295822892066	40.0	37.0	41.0	33.0	41.0
31	37.6629144206674	40.0	36.0	41.0	33.0	41.0
32	37.511453244154396	40.0	36.0	41.0	33.0	41.0
33	37.35265824154032	40.0	35.0	41.0	33.0	41.0
34	37.242203753447875	40.0	35.0	41.0	32.0	41.0
35	37.100834700463324	39.0	35.0	41.0	32.0	41.0
36	37.00203356829941	39.0	35.0	41.0	32.0	41.0
37	36.7816906740702	39.0	35.0	41.0	31.0	41.0
38	36.56013629234347	39.0	35.0	41.0	30.0	41.0
39	36.405000991544824	39.0	35.0	41.0	30.0	41.0
40	36.10119165660098	39.0	35.0	41.0	29.0	41.0
41	35.94731471632804	39.0	35.0	41.0	29.0	41.0
42	35.68153382970668	39.0	35.0	41.0	27.0	41.0
43	34.86808848185473	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	0.0
14	1.0
15	1.0
16	2.0
17	4.0
18	2.0
19	18.0
20	39.0
21	70.0
22	158.0
23	293.0
24	450.0
25	827.0
26	1168.0
27	1726.0
28	2545.0
29	3511.0
30	4667.0
31	5884.0
32	7503.0
33	9738.0
34	12562.0
35	16449.0
36	22970.0
37	37922.0
38	61627.0
39	87205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86217526906921	20.288809965926912	12.92974454199643	21.919270223007445
2	18.304638626980836	21.884295732751628	35.911950819376585	23.899114820890947
3	19.90805675242027	26.959202437397465	30.388505291243757	22.744235518938506
4	12.438659431394113	17.09423281472534	34.44121941985613	36.02588833402441
5	11.572590095368584	41.34273197641926	34.31285943499973	12.771818493212425
6	30.988119490165676	35.61016063026195	17.087382141376263	16.314337738196112
7	27.16977050244281	32.57999963943824	22.07467233950495	18.175557518614
8	29.121491283419566	32.587931998052966	19.626097459842434	18.66447925868503
9	26.83877481115578	12.272079900484956	21.244659179000884	39.64448610935838
10	18.940669563179434	28.52367989327372	30.86588905514792	21.669761488398926
11	32.131100254196035	23.886495159458438	20.937100001802808	23.045304584542716
12	20.877607312192396	30.623591555643692	27.07674556959743	21.422055562566477
13	29.573275162703496	20.496132975175325	27.254502514918244	22.676089347202943
14	23.024392002740267	22.263967260992626	27.544394166110802	27.167246570156305
15	26.648398204402458	27.00463321855451	25.286917016712035	21.060051560330994
16	24.004759415168834	25.6608195568696	27.307144531179578	23.027276496781987
17	20.563918585155673	29.752113793289947	27.157150841010292	22.526816780544088
18	19.990625394364418	25.94061547891615	30.15233734157818	23.91642178514125
19	21.584668914168272	26.776758189258864	31.35156573942202	20.287007157150843
20	21.640195424471326	25.509023057924246	31.9699291496151	20.880852367989327
21	23.057203122464802	26.2943265607817	29.88191602516721	20.766554291586292
22	23.203230633326726	27.249815212100454	28.77535199841353	20.771602156159297
23	22.27189961960735	27.065207593430564	29.93996646775676	20.722926319205325
24	21.441886459103284	28.272368349889128	28.58317258288413	21.702572608123457
25	21.760262488957796	27.523842146063565	29.77374749860282	20.942147866375812
26	21.201752330130343	28.470316753501955	29.63312841406912	20.694802502298582
27	20.950080224990536	29.141682741711588	28.668986280625212	21.239250752672664
28	20.21056806504534	27.904234797814997	31.475959544971065	20.4092375921686
29	21.17182570444753	28.524761578539366	30.733202329228938	19.57021038778417
30	20.420054444825038	28.883881086733133	30.643422452180495	20.052642016261334
31	19.872721700409237	29.849465467197895	29.320881934053254	20.956930898339614
32	19.876327317961383	28.827994014674864	30.220483513313745	21.07519515405001
33	19.603742631019127	28.90875984784294	30.964322414321515	20.523175106816417
34	20.44529376769006	28.52656438731544	30.06508139681624	20.96306044817826
35	20.042185725360113	28.92534568858281	30.354973048008798	20.67749553804828
36	20.196866718347184	29.661252230975858	28.1238169067407	22.018064143936254
37	19.316014350357857	28.192323640231482	30.298004290684887	22.193657718725778
38	19.404712542140658	27.546918098397306	30.947376011826428	22.100993347635615
39	19.22443166453334	28.412266310912404	30.506048423443726	21.85725360111053
40	18.81555463411996	27.984640069227858	31.72186266202744	21.477942634624743
41	18.715679027925507	27.831761885016853	30.51758639961059	22.934972687447043
42	18.047197533757593	28.431376083938776	31.452883592637328	22.068542789666303
43	18.683589031711406	27.155348032234222	31.30649552002019	22.85456741603418
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	29.0
2	40.0
3	162.5
4	285.0
5	285.0
6	503.0
7	721.0
8	758.0
9	795.0
10	1113.5
11	1432.0
12	1432.0
13	2600.0
14	3768.0
15	5648.5
16	7529.0
17	6691.0
18	5853.0
19	5853.0
20	6376.5
21	6900.0
22	5106.0
23	3312.0
24	3186.5
25	3061.0
26	3061.0
27	3357.5
28	3654.0
29	4463.5
30	5273.0
31	6393.0
32	7513.0
33	7513.0
34	9163.5
35	10814.0
36	11942.5
37	13071.0
38	15410.5
39	17750.0
40	17750.0
41	19135.5
42	20521.0
43	22440.5
44	24360.0
45	23774.5
46	23189.0
47	23189.0
48	23820.0
49	24451.0
50	24472.0
51	24493.0
52	20599.5
53	16706.0
54	16706.0
55	16029.0
56	15352.0
57	12509.0
58	9666.0
59	8466.0
60	7266.0
61	7266.0
62	6609.0
63	5952.0
64	5074.5
65	4197.0
66	3526.5
67	2856.0
68	2856.0
69	2517.5
70	2179.0
71	1884.5
72	1590.0
73	1351.0
74	1112.0
75	1112.0
76	939.5
77	767.0
78	597.5
79	428.0
80	346.0
81	264.0
82	264.0
83	187.0
84	110.0
85	83.5
86	57.0
87	41.0
88	25.0
89	25.0
90	18.5
91	12.0
92	7.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	277345.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.50025605220541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.65736079246896	28.74670658003878
2	10.182369932082567	7.636829593427473
3	3.6054393066808883	4.056146915436577
4	1.9226167132812204	2.883944761531814
5	1.3241505436179957	2.482799221867091
6	0.9826097639820848	2.210887064923518
7	0.6890237000595989	1.8086995624791053
8	0.5552131665133762	1.665650872624589
9	0.45235891625134866	1.5267217668231343
>10	3.1735024960500726	23.43145304140625
>50	0.2925853465361411	7.484569007310984
>100	0.15278347447109072	10.201736802230107
>500	0.0039943392018585815	0.976622603245462
>1k	0.005991508802787872	4.887232206655109
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3439	1.2399718761830931	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3302	1.1905749157186898	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2228	0.8033315906181832	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1493	0.5383187005354342	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	1407	0.5073103895869765	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	1182	0.426183994663686	RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	759	0.2736663722078999	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	711	0.25635940795759793	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	636	0.22931727631650112	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	502	0.18100200111774142	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	404	0.14566694910670824	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	391	0.14097964628891813	No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG	380	0.13701346698155725	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	374	0.13485009645026952	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	369	0.1330472876741964	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	356	0.1283599848564063	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	353	0.1272782995907624	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	333	0.12006706448646992	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG	323	0.11646144693432368	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	321	0.11574032342389443	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	320	0.1153797616686798	No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT	317	0.11429807640303592	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	316	0.1139375146478213	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	313	0.11285582938217745	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	311	0.11213470587174817	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	294	0.10600515603309955	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0010816852656438732	0.0
3	0.0	0.0	0.0	0.0021633705312877464	0.0
4	0.0	0.0	0.0	0.002523932286502371	0.0
5	0.0	0.0	0.0	0.002523932286502371	0.0
6	0.0	0.0	0.0	0.002523932286502371	0.0
7	0.0	0.0	0.0	0.0032450557969316194	0.0
8	0.0	0.0	0.0	0.0032450557969316194	0.0
9	0.0	0.0	0.0	0.005047864573004742	0.0
10	0.0	0.0	0.0	0.00576898808343399	0.0
11	0.0	0.0	3.6056175521462436E-4	0.006490111593863239	0.0
12	0.0	0.0	3.6056175521462436E-4	0.007571796859507112	0.0
13	0.0	0.0	3.6056175521462436E-4	0.008292920369936362	0.0
14	0.0	0.0	3.6056175521462436E-4	0.009374605635580234	0.0
15	0.0	0.0	3.6056175521462436E-4	0.0147830319637996	0.0
16	0.0	0.0	3.6056175521462436E-4	0.02235482882330671	0.0
17	0.0	0.0	3.6056175521462436E-4	0.03461392850060394	0.0
18	0.0	0.0	3.6056175521462436E-4	0.042546287115325675	0.0
19	0.0	0.0	3.6056175521462436E-4	0.04939696046440354	0.0
20	0.0	0.0	3.6056175521462436E-4	0.05480538679262291	0.0
21	0.0	0.0	7.211235104292487E-4	0.06742504822513476	0.0
22	0.0	0.0	7.211235104292487E-4	0.09446717986623158	0.0
23	0.0	0.0	7.211235104292487E-4	0.13340784942941103	0.0
24	0.0	0.0	7.211235104292487E-4	0.20660188573797977	0.0
25	0.0	0.0	7.211235104292487E-4	0.22426941174349638	0.0
26	0.0	0.0	7.211235104292487E-4	0.24770592583244697	0.0
27	0.0	0.0	7.211235104292487E-4	0.2675368223692513	0.0
28	0.0	0.0	7.211235104292487E-4	0.30251131262506986	0.0
29	0.0	0.0	7.211235104292487E-4	0.35262939659990267	0.0
30	0.0	0.0	7.211235104292487E-4	0.46836972002379706	0.0
31	0.0	0.0	7.211235104292487E-4	0.8815734914997566	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	25	1.2312114E-4	37.0	37
GTAAGCC	20	0.0018395288	37.0	9
AGGAGTG	30	3.592501E-4	30.833332	5
GCCGTCT	140	0.0	30.392859	36
TTTAGCG	25	0.0054892437	29.6	26
ATGCACC	25	0.0054892437	29.6	4
CTAGGGG	25	0.0054892437	29.6	18
CCGTCTT	145	0.0	29.344828	37
GTACGGA	45	1.3196781E-4	24.666666	6
GTGTGAT	55	1.895918E-5	23.545454	3
CATCGTA	40	0.0019275139	23.125002	24
TATTAGC	65	2.6706748E-6	22.76923	2
TACTTGT	50	2.6949443E-4	22.2	16
CCTAGTG	50	2.6949443E-4	22.2	17
AAGGTTT	50	2.6949443E-4	22.2	7
TGTGATA	50	2.6949443E-4	22.2	4
ATAGTAC	70	5.078922E-6	21.142859	3
GTATTAG	150	0.0	20.966667	1
ACTTTTA	80	6.921E-7	20.8125	36
GAGGCAC	80	6.921E-7	20.8125	7
>>END_MODULE