FastQCFastQC Report
Fri 10 Feb 2017
ERR1630820.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630820.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997315
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11970.12002225976747569No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10770.10798995302386909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.024.3583341
TAGGACG400.00193100923.1254
CTATACA450.003825458220.5555554
TAGGTAC555.1424373E-420.18181822
GTACTAA555.1424373E-420.1818181
TTAGGAC2550.019.5882363
TTAATAC1306.9667294E-1018.53
TATAGGA902.1516353E-618.52
GTATAGG953.605419E-617.5263161
TAACGGC852.7225296E-517.41176436
TAGGACA2550.017.4117644
TAACACG752.0669203E-417.2666664
TATTAAG650.001579735517.0769232
GGTCTAG650.001579735517.0769231
CTAGCGG1554.0017767E-1016.70967729
TCTTATA7250.016.58620637
GCTTAGA904.4450073E-516.4444451
GTATAAA1251.6569538E-716.2799991
ACGGTAT1151.2417149E-616.0869569
TAATACT1403.4709956E-815.8571424
Produced by FastQC (version 0.11.5)