FastQCFastQC Report
Fri 10 Feb 2017
ERR1630819.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630819.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704896
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111671.584205329580534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT93411.3251600236063192No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80361.140026330125295No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56780.8055088977664789No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA18640.2644361721445433No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC15680.22244416197566733No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15510.22003245868894133No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC15390.21833007989831124No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14720.2088251316506265No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14460.20513664427092793No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA13200.1872616669693118No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT12970.18399877428727077No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC12770.18116147630288723No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC12630.17917536771381878No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT12230.17350077174505177No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC11890.16867736517159979No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG11650.16527260759033957No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC11390.161584120210641No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG11310.16044920101688762No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA10750.15250476666061374No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC10400.14753949518794263No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT10360.14697203559106592No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG10270.14569525149809334No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC10270.14569525149809334No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC10030.14229049391683313No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC9680.13732522244416198No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9580.13590657345197021No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG9520.13505538405665518No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC9050.12838773379335391No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG8950.12696908480116217No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT8940.12682721990194298Illumina PCR Primer Index 10 (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8760.12427365171599782No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT8480.12030143453786091No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT8400.1191665153441075No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8260.11718040675503905No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8220.11661294715816235No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA8200.11632921735972399No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC8160.11576175776284729No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG7960.11292445977846377No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG7770.11022902669329943No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC7710.10937783729798438No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.105831214817505No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7460.105831214817505No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC7440.10554748501906663No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7130.10114967314327221No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG7070.10029848374795715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACT451.07895175E-732.888891
ACATTCC303.5980783E-430.8333348
ACCGCAT358.865978E-426.4285713
GTATAGA701.9207255E-723.7857131
TAACTAC551.9013052E-523.5454545
TCACAGG400.001930449623.1250028
GCATTGA400.001930449623.12500235
CACATGT2450.022.6530628
ATACTCT502.7006652E-422.26
GTGACAC2450.021.89795924
TTGCCGA603.7236543E-521.58333410
CCGTCTT1650.021.3030337
GCCGTCT1650.021.3030336
ACACATG2550.021.03921527
ACAGGCG1151.3460522E-1020.91304425
ACATGTT2500.020.7229
GACACAT2600.020.63461526
AGTATAC450.003824361120.5555572
CTAGTTG450.003824361120.55555714
ATTTCAG450.003824361120.55555727
Produced by FastQC (version 0.11.5)