FastQCFastQC Report
Fri 10 Feb 2017
ERR1630818.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630818.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559684
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108691.9419886936199713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90111.6100156516891677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62911.124027129594557No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44740.7993796499453263No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT26300.46990801952530353No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT16080.28730497923828446RNA PCR Primer, Index 26 (100% over 22bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13020.23263127050264076No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11590.2070811386425197No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11520.20583043288712916No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA10820.19332337533322375No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC9560.170810671736194No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG9500.16973863823157354No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9480.16938129373003338No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9390.16777324347310268No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC9030.1613410424453799No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC8490.1516927409037957No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT8350.14919132939301463No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.14579655662838314No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC7990.14275912836529184No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA7720.13793497759449974No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC7510.13418286032832813No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT7470.13346817132524783No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC7350.13132410431600688No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7280.13007339856061634No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG6850.12239049177750302No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6830.12203314727596286No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA6550.1170303242544007No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA6530.11667297975286055No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC6480.11577961849901015No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC6410.11452891274361962No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6390.11417156824207945No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG6370.1138142237405393No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC6220.11113413997898813No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG6110.10916874522051728No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA6090.10881140071897714No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG6070.10845405621743699No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6050.10809671171589683No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG5930.1059526447066559No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5780.10327256094510473No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5720.10220052744048427No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC5690.10166451068817405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTTA200.001841129337.03
AGGGGTT451.0782787E-732.888895
TGGCCAG303.5971397E-430.83333225
CTCATGG250.00549396429.637
CGTCTTC556.252103E-726.9090937
CCGTCTT2350.026.76595537
GGTACTT358.863684E-426.4285722
GCCGTCT2400.026.20833236
CAAATTC2050.023.46341330
AGTCCTT502.6997025E-422.212
GTGTCCA753.7336758E-722.19999921
CCGCTTA855.1688403E-821.76470625
ATCTTAC1059.804353E-1021.1428571
CCTAGTG806.9469934E-720.812517
TAGTGTC806.9469934E-720.812519
CTTGACT450.003823391420.5555572
GCAGAAC1351.8189894E-1220.5555553
TGTTTAC1101.7498678E-920.18181814
GTCTGGG555.1385624E-420.18181811
ATGTAGG555.1385624E-420.1818181
Produced by FastQC (version 0.11.5)