FastQCFastQC Report
Fri 10 Feb 2017
ERR1630817.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630817.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677806
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43380.2585519422388524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39810.23727415446124284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36490.2174864078445303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24460.14578562718216528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT502.7028192E-422.26
TACACCG656.904548E-519.9230775
GGTATCA19850.018.1738031
GCGTTAT1850.018.01
CGTTTAG700.002593159415.85714326
CGTTATT2003.6379788E-1215.7252
CGACGGT2600.015.6538467
CGAACTA957.062766E-515.57894829
GTATAGA1557.214112E-915.5161291
CTTATAC10100.015.01980137
TAGACTG1853.0559022E-1015.05
GTAGCAC1001.0938393E-414.83
TCTTATA15650.014.65814737
GTATTAC1651.6463673E-814.5757571
ACGGTAT2800.014.5357159
TACGACG2950.014.4237295
GTATTAG4250.014.3647061
GTCTTAC1051.6563477E-414.0952381
AAGACGG2500.014.0599995
GACGGTA2900.014.0344848
Produced by FastQC (version 0.11.5)