##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630817.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1677806
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.018817431812735	33.0	31.0	34.0	30.0	34.0
2	32.190753877385106	33.0	31.0	34.0	30.0	34.0
3	32.305261156534186	34.0	31.0	34.0	30.0	34.0
4	35.89131997382296	37.0	35.0	37.0	35.0	37.0
5	35.865880799091194	37.0	35.0	37.0	35.0	37.0
6	35.89511123455274	37.0	35.0	37.0	35.0	37.0
7	35.87289829694255	37.0	35.0	37.0	35.0	37.0
8	35.86472333511741	37.0	35.0	37.0	35.0	37.0
9	37.61018496774955	39.0	37.0	39.0	35.0	39.0
10	37.518081947495716	39.0	37.0	39.0	35.0	39.0
11	37.6179969555479	39.0	37.0	39.0	35.0	39.0
12	37.54937698398981	39.0	37.0	39.0	35.0	39.0
13	37.59225202436992	39.0	37.0	39.0	35.0	39.0
14	38.88243336833937	40.0	38.0	41.0	36.0	41.0
15	38.856429169999394	40.0	38.0	41.0	35.0	41.0
16	38.84014480816018	40.0	38.0	41.0	35.0	41.0
17	38.82516870246024	40.0	38.0	41.0	35.0	41.0
18	38.81074808410508	40.0	38.0	41.0	35.0	41.0
19	38.84457082642451	40.0	38.0	41.0	35.0	41.0
20	38.816309513734005	40.0	38.0	41.0	35.0	41.0
21	38.75015585830543	40.0	38.0	41.0	35.0	41.0
22	38.69863977122504	40.0	38.0	41.0	34.0	41.0
23	38.67864103477994	40.0	38.0	41.0	34.0	41.0
24	38.62398811304764	40.0	38.0	41.0	34.0	41.0
25	38.58419507380472	40.0	38.0	41.0	34.0	41.0
26	38.4110135498383	40.0	38.0	41.0	34.0	41.0
27	38.31054067037548	40.0	38.0	41.0	34.0	41.0
28	38.23036095949114	40.0	38.0	41.0	34.0	41.0
29	38.134033374537935	40.0	38.0	41.0	33.0	41.0
30	38.064981887059645	40.0	38.0	41.0	33.0	41.0
31	38.02365112533869	40.0	37.0	41.0	33.0	41.0
32	37.92744572376067	40.0	37.0	41.0	33.0	41.0
33	37.91426839575016	40.0	37.0	41.0	33.0	41.0
34	37.84531286692263	40.0	37.0	41.0	33.0	41.0
35	37.7943844520761	40.0	37.0	41.0	33.0	41.0
36	37.761884270291084	40.0	37.0	41.0	33.0	41.0
37	37.58935836443546	40.0	37.0	41.0	33.0	41.0
38	37.49153120205793	40.0	37.0	41.0	32.0	41.0
39	37.44633229348327	40.0	37.0	41.0	32.0	41.0
40	37.35978951082545	40.0	36.0	41.0	32.0	41.0
41	37.24397933968528	40.0	36.0	41.0	31.0	41.0
42	37.1721533955654	40.0	36.0	41.0	31.0	41.0
43	36.34468645361859	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	3.0
12	2.0
13	0.0
14	5.0
15	5.0
16	11.0
17	23.0
18	44.0
19	101.0
20	201.0
21	363.0
22	750.0
23	1241.0
24	2086.0
25	3367.0
26	5140.0
27	7677.0
28	11404.0
29	16181.0
30	21905.0
31	29548.0
32	38812.0
33	51465.0
34	68859.0
35	93692.0
36	132464.0
37	204430.0
38	378571.0
39	609451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.328430104553206	19.157518807299535	12.104557976309538	25.409493111837723
2	18.401412320613943	21.045162551570325	35.264923358242854	25.288501769572886
3	20.09654274689684	23.65625108027984	29.268997726793206	26.97820844603011
4	14.430393025176928	15.502626644558429	34.6625295177154	35.40445081254925
5	14.187158706072097	37.05523761388385	34.56800130646809	14.189602373575969
6	34.72993897983438	34.94212084114612	15.879547456618942	14.448392722400563
7	28.977128464196696	30.364416386638265	21.908492400194064	18.749962748970976
8	26.666313030231148	33.91125076439111	20.41952406893288	19.00291213644486
9	26.579354228081197	13.967288232370132	20.023709534952193	39.42964800459648
10	16.61521057857702	26.72978878368536	33.45494055927801	23.200060078459607
11	35.13350172785173	21.80967287040337	22.068165210995787	20.98866019074911
12	21.486035930256538	25.39709596937906	29.554251206635335	23.56261689372907
13	29.501682554478887	20.10333733459053	25.928623452294246	24.466356658636336
14	22.84769514473068	21.102737742027387	25.59270857298162	30.456858540260317
15	25.39053978827111	27.749394149263978	22.777007592057725	24.083058470407188
16	25.081326446561757	26.72150415483077	24.309663930156404	23.88750546845106
17	23.423268244361985	26.696709869913448	25.98894031848736	23.89108156723721
18	23.3301108709827	25.55259666493027	27.247071473102373	23.870220990984656
19	24.060529048054423	25.6436679806843	27.041326589605713	23.254476381655568
20	24.510581080291765	24.98161289207453	26.92432855765208	23.58347746998163
21	24.66542615773218	25.514690017797054	26.123282429553836	23.696601394916932
22	24.484117949274232	25.220973104161033	26.335166282633395	23.95974266393135
23	23.816639110838796	25.522259426894404	26.647955723128895	24.013145739137897
24	23.9734510426116	25.855134622238808	26.472726882607407	23.69868745254219
25	24.341729615938913	25.340116795386358	26.433747405838336	23.884406182836397
26	23.862413175301555	26.287186957252505	26.409370332446063	23.441029534999874
27	24.48280671305264	25.464803439730222	25.89846501919769	24.15392482801945
28	23.60505326599142	25.709110588470896	26.762986900750146	23.92284924478754
29	23.334819401051135	25.986079439458436	26.921706085208896	23.757395074281533
30	23.2933962567782	26.45872049569497	26.82866791512249	23.41921533240434
31	23.93703443663928	26.143070176170546	26.173467015852847	23.74642837133733
32	23.222947110690985	25.84124743861925	26.46962759699274	24.46617785369703
33	23.201848127852685	25.75101054591532	26.865263326034118	24.181878000197877
34	24.03960887015543	25.32372634261649	26.776099262966042	23.860565524262043
35	23.86998258439891	25.507299413639	27.243793382548397	23.378924619413688
36	23.293873069949683	25.871703879947976	26.80446964666952	24.029953403432817
37	23.908425646350054	25.004261517720167	26.96360604265332	24.123706793276458
38	23.287495693781047	25.011115707060295	27.62524391973804	24.076144679420626
39	23.377792188131405	25.02834058288026	27.368301221952958	24.22556600703538
40	22.824569705913554	25.02589691537639	28.041025005274744	24.10850837343531
41	22.280347072307524	24.962540365215048	28.587154891566723	24.1699576709107
42	23.013149315236685	24.833323995742056	28.195870082715164	23.957656606306095
43	22.342273182954404	24.390304957784153	28.440952052859508	24.82646980640193
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	270.0
1	261.5
2	253.0
3	668.5
4	1084.0
5	1084.0
6	1420.0
7	1756.0
8	1673.0
9	1590.0
10	2304.5
11	3019.0
12	3019.0
13	4929.5
14	6840.0
15	9176.5
16	11513.0
17	12043.0
18	12573.0
19	12573.0
20	14966.0
21	17359.0
22	18282.0
23	19205.0
24	21858.0
25	24511.0
26	24511.0
27	28012.5
28	31514.0
29	36875.0
30	42236.0
31	48526.0
32	54816.0
33	54816.0
34	60241.5
35	65667.0
36	71750.5
37	77834.0
38	85109.0
39	92384.0
40	92384.0
41	95841.5
42	99299.0
43	99333.5
44	99368.0
45	102997.0
46	106626.0
47	106626.0
48	108515.0
49	110404.0
50	114244.0
51	118084.0
52	122281.0
53	126478.0
54	126478.0
55	119444.0
56	112410.0
57	106971.5
58	101533.0
59	92906.0
60	84279.0
61	84279.0
62	78240.5
63	72202.0
64	63945.0
65	55688.0
66	48644.0
67	41600.0
68	41600.0
69	35650.5
70	29701.0
71	26093.5
72	22486.0
73	18016.5
74	13547.0
75	13547.0
76	10515.0
77	7483.0
78	6321.0
79	5159.0
80	4069.0
81	2979.0
82	2979.0
83	2265.5
84	1552.0
85	1331.0
86	1110.0
87	987.5
88	865.0
89	865.0
90	657.0
91	449.0
92	252.0
93	55.0
94	37.0
95	19.0
96	19.0
97	12.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1677806.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.93004573899965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.7905625797384	52.01362385117234
2	7.912280030218312	9.483666082215139
3	2.120950704445828	3.81326018182806
4	0.9388530816817764	2.250620325095585
5	0.5087574107720725	1.5244927448812668
6	0.2964401868009291	1.0659404372314367
7	0.2123552274187617	0.8908520944485441
8	0.16610602044487774	0.7963793122227787
9	0.11797544954491752	0.6363246678575357
>10	0.731713036063474	8.563508594104569
>50	0.10321657920385385	4.416331677409415
>100	0.09370469126841884	10.964447680927494
>500	0.006386481035116615	2.506601937855679
>1k	6.985213632158797E-4	1.0739504127502095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4338	0.2585519422388524	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3981	0.23727415446124284	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3649	0.2174864078445303	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2446	0.14578562718216528	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.364148179229303E-4	0.0
3	0.0	0.0	0.0	9.536263429740983E-4	0.0
4	0.0	0.0	0.0	0.0018476510395123156	0.0
5	0.0	0.0	0.0	0.0020860576252558403	0.0
6	0.0	0.0	0.0	0.0020860576252558403	0.0
7	0.0	0.0	0.0	0.002562870796742889	0.0
8	0.0	0.0	0.0	0.0029800823217940573	0.0
9	0.0	0.0	0.0	0.004827733361306373	0.0
10	0.0	0.0	0.0	0.0075694090973569056	0.0
11	0.0	0.0	0.0	0.00888064531894629	0.0
12	0.0	0.0	0.0	0.00941706013686922	0.0
13	0.0	0.0	0.0	0.010191881540535675	0.0
14	0.0	0.0	0.0	0.010787898004894486	0.0
15	0.0	0.0	0.0	0.011801125994304466	0.0
16	0.0	0.0	0.0	0.013112362215893852	0.0
17	0.0	0.0	0.0	0.01561563136620086	0.0
18	0.0	0.0	0.0	0.016688461002046722	0.0
19	0.0	0.0	0.0	0.01823810380937963	0.0
20	0.0	0.0	0.0	0.020085754848891947	0.0
21	0.0	0.0	0.0	0.022231414120583666	0.0
22	0.0	0.0	0.0	0.025926716199608298	0.0
23	0.0	5.960164643588114E-5	0.0	0.03301931212547815	0.0
24	0.0	5.960164643588114E-5	0.0	0.043032388726706186	0.0
25	0.0	5.960164643588114E-5	0.0	0.046966097391474344	0.0
26	0.0	5.960164643588114E-5	0.0	0.05304546532793422	0.0
27	0.0	5.960164643588114E-5	0.0	0.06651543742244335	0.0
28	0.0	5.960164643588114E-5	0.0	0.09697187875117862	0.0
29	0.0	5.960164643588114E-5	0.0	0.14232873168888419	0.0
30	0.0	5.960164643588114E-5	0.0	0.20884416911132753	0.0
31	0.0	5.960164643588114E-5	0.0	0.45184008163041495	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCGT	50	2.7028192E-4	22.2	6
TACACCG	65	6.904548E-5	19.923077	5
GGTATCA	1985	0.0	18.173803	1
GCGTTAT	185	0.0	18.0	1
CGTTTAG	70	0.0025931594	15.857143	26
CGTTATT	200	3.6379788E-12	15.725	2
CGACGGT	260	0.0	15.653846	7
CGAACTA	95	7.062766E-5	15.578948	29
GTATAGA	155	7.214112E-9	15.516129	1
CTTATAC	1010	0.0	15.019801	37
TAGACTG	185	3.0559022E-10	15.0	5
GTAGCAC	100	1.0938393E-4	14.8	3
TCTTATA	1565	0.0	14.658147	37
GTATTAC	165	1.6463673E-8	14.575757	1
ACGGTAT	280	0.0	14.535715	9
TACGACG	295	0.0	14.423729	5
GTATTAG	425	0.0	14.364706	1
GTCTTAC	105	1.6563477E-4	14.095238	1
AAGACGG	250	0.0	14.059999	5
GACGGTA	290	0.0	14.034484	8
>>END_MODULE