FastQCFastQC Report
Fri 10 Feb 2017
ERR1630816.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630816.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035944
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT214702.0725058497370514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT200691.9372668792907726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT160221.5466086969952044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97880.9448387171507341No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24870.24007089186288064No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24390.23543743677264406No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT24390.23543743677264406No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20060.1936398106461353No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17830.17211355053941138No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT15610.1506838207470674RNA PCR Primer, Index 17 (95% over 21bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA14950.14431281999799217No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT14560.14054813773717498No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13440.12973674252662307No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA12810.1236553327206876No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT12500.12066289297490984No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12490.12056636266052992No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC12030.11612596819905324No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12000.11583637725591345No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC11630.11226475562385611No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC11540.11139598279443677No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC11190.10801742179113928No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT10580.10212907261396369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC451.3230887E-424.6666683
GCCGTCT1700.023.94117736
TCTACAC603.7258305E-521.5833323
ACACCGC450.003825556320.5555576
TAGCACG450.003825556320.5555574
CGAATGT450.003825556320.5555574
CCGTCTT2000.020.3537
CAGTCGG2950.020.06779710
GGTAAAC1303.274181E-1119.92307535
GCAGTCG3100.019.6935489
TCTATAC1052.258821E-819.3809533
CTATACA500.007034423818.54
GGTATTG500.007034423818.533
ACTGTGC609.235979E-418.58
GCAGCGT609.235979E-418.51
CGTTTGG609.235979E-418.528
AGTCGGT3150.018.20634811
GGTATCA79600.018.1978651
TCCTCGC2350.018.10638221
GGAACGT953.60569E-617.5263168
Produced by FastQC (version 0.11.5)